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p65 antibody (Nuclear Factor-KB P65)

Details for Product anti-NFkBP65 Antibody No. ABIN969311, Supplier: Log in to see
Antigen
  • NFKB3
  • p65
  • NFkB
Alternatives
anti-Human p65 antibody for Western Blotting
Reactivity
Human, Mouse (Murine)
1024
479
395
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37
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12
10
6
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2
2
1
1
1
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1
Host
Mouse
952
78
13
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
This p65 antibody is un-conjugated
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30
22
19
16
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11
9
9
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8
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8
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
745
399
337
163
130
129
90
33
30
21
20
17
11
9
7
7
6
4
2
2
1
1
Supplier
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Immunogen Purified recombinant fragment of human NF-kappaB p65 expressed in E. coli.
Clone 6H7
Isotype IgG1
Purification Ascites
Alternative Name NF-kappa B p65 (NFkBP65 Antibody Abstract)
Background Transcription factors of the nuclear factor κ B (NF-κB)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). The most studied NF-κB complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-κB with its inhibitor, IκB. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IκB. This complex prevents nuclear translocation and activity of NF-κB. In response to stimuli such as cytokines, LPS, and viral infections, IκB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IκB/NF-κB complex, allowing the free heterodimeric NF-κB to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the κB site within promoters and enhancers and functions as a transcriptional activator.
Molecular Weight 65 kDa
Gene ID 5970
HGNC 5970
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Western Blotting (WB) image for anti-p65 antibody (Nuclear Factor-KB P65) (ABIN969311) Figure 1: Western blot analysis using NF-κB p65 mouse mAb against Jurkat (1), K562 (2...
Product cited in: Yamit-Hezi, Nir, Wolstein et al.: "Interaction of TAFII105 with selected p65/RelA dimers is associated with activation of subset of NF-kappa B genes." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18180-7, 2000 (PubMed).

Background publications DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

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