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SMAD5 antibody (SMAD, Mothers Against DPP Homolog 5)

Details for Product anti-SMAD5 Antibody No. ABIN969404, Supplier: Log in to see
Antigen
  • 1110051M15Rik
  • AI451355
  • DKFZp468M0619
  • dpc4
  • Dwf-C
  • DWFC
  • fb67b04
  • jip
  • JV5-1
  • jv5-1
  • mad
  • madh4
  • Madh5
  • madh5
  • MADH5
  • MusMLP
  • sb:cb400
  • SBN
  • smad5
  • SMAD5
  • smad10
  • smd5
  • wu:fb67b04
  • xsmad4
  • XSmad4alpha
Reactivity
Human, Rat (Rattus)
92
28
25
4
2
1
1
1
1
1
Host
Mouse
62
31
1
Clonality (Clone)
Monoclonal ()
Conjugate
This SMAD5 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
85
49
21
17
14
11
6
1
1
1
1
Supplier
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Immunogen Purified recombinant fragment of human SMAD5 expressed in E. coli.
Clone 3H9
Isotype IgG1
Purification Ascites
Alternative Name SMAD5 (SMAD5 Antibody Abstract)
Background Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo, lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.
Molecular Weight 52 kDa
Gene ID 4090
HGNC 4090
Research Area Organogenesis, Signaling, Transcription Factors
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-SMAD5 antibody (SMAD, Mothers Against DPP Homolog 5) (ABIN969404) Figure 1: Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and ...
Immunocytochemistry (ICC) image for anti-SMAD5 antibody (SMAD, Mothers Against DPP Homolog 5) (ABIN969404) Figure 2: Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green) ...
Immunohistochemistry (IHC) image for anti-SMAD5 antibody (SMAD, Mothers Against DPP Homolog 5) (ABIN969404) Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and ...
Western Blotting (WB) image for anti-SMAD5 antibody (SMAD, Mothers Against DPP Homolog 5) (ABIN969404) Figure 4: Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), ...
Product cited in: Chang, Chang, Lee, Lee, Yeh, Yeh, Cheng, Chien, Chiu: "Tumor cell cycle arrest induced by shear stress: Roles of integrins and Smad." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 10, pp. 3927-32, 2008

Monzen, Ito, Naito, Kasai, Hiroi, Hayashi, Shiojima, Yamazaki, Miyazono, Asashima, Nagai, Komuro: "A crucial role of a high mobility group protein HMGA2 in cardiogenesis." in: Nature cell biology, Vol. 10, Issue 5, pp. 567-74, 2008