Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) antibody

Antigen: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)

Synonyms: BP-1, 4EBP1, 4E-BP1, PHAS-I, MGC4316, 4e-bp1, AA959816, 153432_at, 43-BP, 4E-BP, 4EBP, BcDNA.HL08053, BcDNA:HL08053, CT8705, Dm 4E-BP, Phas1, anon-WO0172774.158, d4E-BP, d4E-BP1, d4EBP, eIF4E-BP, l(2)k07736, l(2)k13517, pp20, thor, DmelCG8846, CG8846, EIF4EBP1, fc04h09, Eif4ebp1, MGC64137, wu:fc04h09

Clonality: Monoclonal (11G12C11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN969467

Additional Information

Alternative name: 4E-BP1

Gene ID: 1978

Immunogen: Purified recombinant fragment of 4EBP1 expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 11G12C11

Description: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.
Synonyms: BP-1, 4EBP1, 4E-BP1, PHAS-I, MGC4316, EIF4EBP1

Synonyms: BP-1, 4EBP1, 4E-BP1, PHAS-I, MGC4316, 4e-bp1, AA959816, 153432_at, 43-BP, 4E-BP, 4EBP, BcDNA.HL08053, BcDNA:HL08053, CT8705, Dm 4E-BP, Phas1, anon-WO0172774.158, d4E-BP, d4E-BP1, d4EBP, eIF4E-BP, l(2)k07736, l(2)k13517, pp20, thor, DmelCG8846, CG8846, EIF4EBP1, fc04h09, Eif4ebp1, MGC64137, wu:fc04h09

Application Details

Application Notes: Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Purified

Buffer: PBS containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Restrictions: For Research Use only

Publications

Product: Pause, Belsham, Gingras et al.: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." in: Nature, Vol. 371, Issue 6500, pp. 762-7, 1994 (PubMed).

Fadden, Haystead, Lawrence: "Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10240-7, 1997 (PubMed).