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Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 637-651) antibody

Details for Product No. ABIN969538, Supplier: Log in to see
Antigen
  • MELK
  • xmelk
  • Melk
  • HPK38
  • AI327312
  • MPK38
  • mKIAA0175
  • fb71b01
  • fe01f04
  • wu:fb71b01
  • wu:fe01f04
Epitope
AA 637-651
41
18
13
13
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
109
27
18
6
6
2
2
2
2
2
Host
Mouse
87
20
7
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
6
6
6
5
5
5
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA
86
59
11
8
6
5
5
4
2
2
1
Supplier
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Immunogen Synthesized peptide of human MELK (AA 637-651: C-VYKRLVEDILSSCKV) .
Sequence VYKRLVEDIL SSCKV
Clone 2G2
Isotype IgG2a
Purification Purified
Alternative Name MELK (MELK Antibody Abstract)
Background Maternal embryonic leucine-zipper kinase (MELK) is a key regulator of survival of stemlike GBM cells in vitro. MELK expression is increased in breast cancer tissue and this increase is also associated with poor patient survival, as predicted for a candidate oncogene.
Molecular Weight 74.6 kDa
Gene ID 9833
HGNC 9833
Research Area Embryogenesis, Embryonic stem cells, Hormones, Receptors
Pathways
Application Notes Recommended Dilution:
ELISA: 1/10000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 637-651) antibody (ABIN969538) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 637-651) antibody (ABIN969538) Figure 2: Flow cytometric analysis of MCF-7 cells using MELK mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 637-651) antibody (ABIN969538) Figure 3: Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 637-651) antibody (ABIN969538) Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues usin...
Immunohistochemistry (IHC) image for anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 637-651) antibody (ABIN969538) Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Product cited in: Nakano, Joshi, Visnyei et al.: "Siomycin A targets brain tumor stem cells partially through a MELK-mediated pathway." in: Neuro-oncology, Vol. 13, Issue 6, pp. 622-34, 2011 (PubMed).

Pickard, Green, Ellis et al.: "Dysregulated expression of Fau and MELK is associated with poor prognosis in breast cancer." in: Breast cancer research : BCR, Vol. 11, Issue 4, pp. R60, 2009 (PubMed).