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anti-Mouse (Murine) BID antibody for Flow Cytometry

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • DKFZp469E066
  • FP497
  • fp497
  • si:ch211-238n5.6
  • xbid
Epitope
AA 39-68, Ser61
50
43
34
17
16
14
13
11
10
8
6
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
307
142
63
4
2
2
1
Host
Rabbit
282
90
21
10
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
316
187
120
71
42
34
30
18
13
13
12
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN388100
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2157900 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.0010482287 ABIN2664070 FACS Rat IgG2a Log in to see 14F2 3
0.0010482287 ABIN1841051 FACS IHC (p) WB Rabbit Ser61 Log in to see Polyclonal
0.0010482287 ABIN3030161 FACS IHC ELISA WB Rabbit Ig Fraction AA 39-68 Log in to see Polyclonal
0.0010482287 ABIN2798027 FACS IHC WB Rabbit Ig Fraction Ser61 Log in to see Polyclonal

Top referenced anti-BID antibody for Flow Cytometry

Similar anti-BID Antibodies

Application / Reactivity Mouse (Murine)
ELISA 48 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Western Blotting (WB) 107 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope AA 39-68, Ser61
(50), (43), (34), (17), (16), (14), (13), (11), (10), (8), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(307), (142), (63), (4), (2), (2), (1)
Host Rabbit
(282), (90), (21), (10)
Clonality (Clone)
Polyclonal   ( )
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(316), (187), (120), (71), (42), (34), (30), (18), (13), (13), (12), (3), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID Antibody (ABIN388100) Images
Specificity This mouse BID antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S61 of mouse BID.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This mouse BID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 AA from mouse BID.
Clone RB16438
Isotype Ig

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN388100) Images back to top
Antigen
Alternative Name BID (BID Antibody Abstract)
Background Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.
Synonyms: BH3-interacting domain death agonist, BID, p22 BID
Molecular Weight 21952 DA
Gene ID 12122
NCBI Accession NP_031570
Research Area Apoptosis/Necrosis, Cancer, Cell Structure
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN388100) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN388100) Images back to top
Format Liquid
Concentration 0.47 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-BID Antibody (ABIN388100)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Background publications

Kaufmann, Jost, Pellegrini, Puthalakath, Gugasyan, Gerondakis, Cretney, Smyth, Silke, Hakem, Bouillet, Mak, Dixit, Strasser: "Fatal hepatitis mediated by tumor necrosis factor TNFalpha requires caspase-8 and involves the BH3-only proteins Bid and Bim." in: Immunity, Vol. 30, Issue 1, pp. 56-66, 2009

Ziporen, Donin, Shmushkovich, Gross, Fishelson: "Programmed necrotic cell death induced by complement involves a Bid-dependent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 1, pp. 515-21, 2008

Mandal, Crusio, Meng, Liu, Kinsella, Clark, Takeuchi, Aifantis: "Regulation of lymphocyte progenitor survival by the proapoptotic activities of Bim and Bid." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 52, pp. 20840-5, 2008

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN388100) back to top
Supplier Images
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 39-68) (ABIN388100) Western blot analysis of anti-mouse BID Antibody (S61) (ABIN388100) in mouse spleen t...
Immunohistochemistry (IHC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 39-68) (ABIN388100) mouse BID Antibody (S61)(ABIN388100) IHC analysis in formalin fixed and paraffin embe...
Flow Cytometry (FACS) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 39-68) (ABIN388100) mouse BID Antibody (S61) (ABIN388100) FC analysis of MCF-7 cells (bottom histogram) c...