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anti-Mouse (Murine) BID antibody for Immunofluorescence

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • DKFZp469E066
  • FP497
  • fp497
  • si:ch211-238n5.6
  • xbid
Epitope
AA 46-168
43
34
32
17
16
14
13
11
9
8
6
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
274
128
56
2
1
Host
Mouse
268
68
21
10
Clonality
Monoclonal
Conjugate
This BID antibody is un-conjugated
20
18
13
8
7
7
3
3
3
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
280
166
108
63
34
34
25
18
12
11
9
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN968686
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010850558 ABIN2671500 IF IHC WB Mouse IgG2a Log in to see OTI3E9
0.0010850558 ABIN2959629 IF IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010850558 ABIN3222689 IF IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-BID antibody for Immunofluorescence

  • Human Monoclonal BID Primary Antibody for IF, WB - ABIN968686 (3 Publications): Kudla, Montessuit, Eskes, Berrier, Martinou, Ghazi, Antonsson: The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment. in The Journal of biological chemistry 2000 (PubMed)

Similar anti-BID Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 47 Antibodies 139 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 11 Antibodies
Flow Cytometry (FACS) 6 Antibodies 13 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Western Blotting (WB) 98 Antibodies
Cellular Assay (CA) 1 Antibodies
ELISA (Capture) 1 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope AA 46-168
(43), (34), (32), (17), (16), (14), (13), (11), (9), (8), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(274), (128), (56), (2), (1)
Host Mouse
(268), (68), (21), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BID antibody is un-conjugated
(20), (18), (13), (8), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), Western Blotting (WB)
(280), (166), (108), (63), (34), (34), (25), (18), (12), (11), (9), (3), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID Antibody (ABIN968686) Images
No Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse Bid aa. 46-168
Clone 40-Bid
Isotype IgG1

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN968686) Images back to top
Antigen
Alternative Name Bid (BID Antibody Abstract)
Background Members of the Bcl-2 protein family function to inhibit (Bcl-2, Bcl-XL, Mcl-1, A1) or promote (Bax, Bak, Bcl-Ls, Bad) apoptosis. Bid is a Bcl-2 family death agonist that heterodimerizes with either agonists (Bax) or antagonists (Bcl-2). Bid contains a centrally located BH3 domain that allows interaction with the BH1 domain of Bax and Bcl-2, as well as two caspase cleavage sites (CCS). However, Bid does not contain other domains commonly found in Bcl-2 family members, such as BH1, BH2, or BH4. In addition, Bid does not contain a C-terminal hydrophobic region that is characteristic of membrane-bound Bcl-2 family members. Singlet oxygen-induced apoptosis in human leukemia cells and Fas-induced apoptosis involve caspase-8 cleavage of Bid, which produces a 15 kDa C-terminal fragment and a 6.5 kDa N-terminal fragment. The C-terminal fragment translocates to the mitochondria and promotes the release of cytochrome C through a mechanism that might involve destabilization of the mitochondrial membrane. Thus, the proapoptotic role of Bid may involve mitochondrial membrane destabilization, as well as multiple protein-protein interactions.
Molecular Weight 23 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN968686) Images back to top
Comment

Related Products: ABIN967389, ABIN968550

Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN968686) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-BID Antibody (ABIN968686)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Product cited in:

Kudla, Montessuit, Eskes, Berrier, Martinou, Ghazi, Antonsson: "The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22713-8, 2000

Zhuang, Demirs, Kochevar: "p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 25939-48, 2000

Wang, Yin, Chao, Milliman, Korsmeyer: "BID: a novel BH3 domain-only death agonist." in: Genes & development, Vol. 10, Issue 22, pp. 2859-69, 1996

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN968686) back to top
Supplier Images
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 46-168) (ABIN968686) Western blot analysis of Bid on Mouse Macrophage + IFNgamma/LPS. Lane 1: 1:1000, lane...