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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||47 Antibodies||139 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies||11 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies||13 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Immunohistochemistry (IHC)||36 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||28 Antibodies|
|Immunoprecipitation (IP)||13 Antibodies|
|Western Blotting (WB)||98 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Antigen||BH3 Interacting Domain Death Agonist (BID) Antibodies|
|Epitope||AA 46-168 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This BID antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-BID AntibodyTarget Details BID Application Details Handling References for anti-BID Antibody (ABIN968686) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Mouse Bid aa. 46-168|
Target Details BIDProduct Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN968686) Images back to top
|Alternative Name||Bid (BID Antibody Abstract)|
|Background||Members of the Bcl-2 protein family function to inhibit (Bcl-2, Bcl-XL, Mcl-1, A1) or promote (Bax, Bak, Bcl-Ls, Bad) apoptosis. Bid is a Bcl-2 family death agonist that heterodimerizes with either agonists (Bax) or antagonists (Bcl-2). Bid contains a centrally located BH3 domain that allows interaction with the BH1 domain of Bax and Bcl-2, as well as two caspase cleavage sites (CCS). However, Bid does not contain other domains commonly found in Bcl-2 family members, such as BH1, BH2, or BH4. In addition, Bid does not contain a C-terminal hydrophobic region that is characteristic of membrane-bound Bcl-2 family members. Singlet oxygen-induced apoptosis in human leukemia cells and Fas-induced apoptosis involve caspase-8 cleavage of Bid, which produces a 15 kDa C-terminal fragment and a 6.5 kDa N-terminal fragment. The C-terminal fragment translocates to the mitochondria and promotes the release of cytochrome C through a mechanism that might involve destabilization of the mitochondrial membrane. Thus, the proapoptotic role of Bid may involve mitochondrial membrane destabilization, as well as multiple protein-protein interactions.|
|Molecular Weight||23 kDa|
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN968686) Images back to top
Related Products: ABIN967389, ABIN968550
|Restrictions||For Research Use only|
HandlingProduct Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN968686) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-BID Antibody (ABIN968686)Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
|Product cited in:||
Zhuang, Demirs, Kochevar: "p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 25939-48, 2000 (PubMed).
Kudla, Montessuit, Eskes et al.: "The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22713-8, 2000 (PubMed).
Wang, Yin, Chao et al.: "BID: a novel BH3 domain-only death agonist." in: Genes & development, Vol. 10, Issue 22, pp. 2859-69, 1996 (PubMed).
ImagesProduct Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN968686) back to top