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|Application / Reactivity||Mouse (Murine)|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies|
|Immunohistochemistry (IHC)||45 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||36 Antibodies|
|Immunoprecipitation (IP)||19 Antibodies|
|Western Blotting (WB)||107 Antibodies|
|Antigen||BH3 Interacting Domain Death Agonist (BID) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This BID antibody is un-conjugated Alternatives|
Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-BID AntibodyTarget Details BID Application Details Handling References for anti-BID Antibody (ABIN223028) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412617|
The antibody detects the full length (22 kDa) mouse Bid.
Reactivity to other species has not been tested.
|Immunogen||Synthetic peptide surrounding amino acid 45 of mouse Bid|
Target Details BIDProduct Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN223028) Images back to top
|Alternative Name||Bid (BID Antibody Abstract)|
Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed throμgh Fas.
Synonyms: FP497 , MGC42355, MGC15319
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN223028) Images back to top
Western blot analysis (0.5-5 μg/mL) also works well in Immunoprecipitation (20 μg/mL) and Immunohistochemistry (20 μg/mL).
However, the optimal conditions should be determined individually.
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN223028) Images back to top
|Buffer||100 μg (0.5 mg/mL) affinity purified rabbit anti-Bid polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-BID Antibody (ABIN223028)Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
|Product cited in:||
Huang, Masselli, Frisch, Hunton, Jiang, Wang: "Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb." in: The Journal of biological chemistry, Vol. 282, Issue 40, pp. 29401-13, 2007
ImagesProduct Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN223028) back to top