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anti-Rat (Rattus) BID antibody for Immunohistochemistry (Frozen Sections)

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist-like
  • BH3-interacting domain death agonist-like
  • BID
  • bida
  • bid
  • Bid
  • LOC100355991
  • LOC100730546
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
318
171
73
8
5
3
2
Host
Rabbit
292
94
20
9
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
343
185
124
83
52
41
32
20
13
11
10
6
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN4284613
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4284615 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 3
1 ABIN957167 IHC (fro) IHC (p) IP WB Rabbit AA 1-55 Log in to see Polyclonal
1 ABIN957166 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN1858137 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 1-196 Log in to see
1 ABIN4284614 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN4284612 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN537391 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2

Top referenced anti-BID antibody for Immunohistochemistry (Frozen Sections)

Similar anti-BID Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 12 Antibodies 57 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 4 Antibodies
Immunofluorescence (IF) 3 Antibodies 7 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 27 Antibodies
Immunohistochemistry (IHC) 25 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 45 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 6 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(318), (171), (73), (8), (5), (3), (2)
Host Rabbit
(292), (94), (20), (9)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(343), (185), (124), (83), (52), (41), (32), (20), (13), (11), (10), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling References for anti-BID Antibody (ABIN4284613) Images
Specificity Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by capspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites, generally 11-15 kDa C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.
Purification Unpurified
Immunogen A recombinant protein fragment corresponding to amino acids 1-55 of mouse Bid was used as immunogen.

Target Details BID

Product Details anti-BID Antibody Application Details Handling References for anti-BID Antibody (ABIN4284613) Images back to top
Antigen
Alternative Name BID (BID Antibody Abstract)
Background Gene Symbol: BID
Gene ID 637
Research Area Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BID Antibody Target Details BID Handling References for anti-BID Antibody (ABIN4284613) Images back to top
Application Notes Western Blot 1:1000 - 1:2000, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin 1:1000 - 1:5000, Immunohistochemistry-Frozen Assay DependentThis antibody is useful for immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen .
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details References for anti-BID Antibody (ABIN4284613) Images back to top
Format Liquid
Buffer Whole antisera
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-BID Antibody (ABIN4284613)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Product cited in:

Krajewska, Rosenthal, Mikolajczyk, Stennicke, Wiesenthal, Mai, Naito, Salvesen, Reed, Fiskum, Krajewski: "Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation." in: Experimental neurology, Vol. 189, Issue 2, pp. 261-79, 2004 Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling References for anti-BID Antibody (ABIN4284613) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN4284613) Immunohistochemistry-Paraffin: Bid Antibody [ABIN42846137] - Six representative cores...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN4284613) Immunohistochemistry-Paraffin: Bid Antibody [ABIN42846137] - Formalin-fixed, paraffin...