anti-Rat (Rattus) BID antibody for Immunoprecipitation

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist-like
  • BH3-interacting domain death agonist-like
  • BID
  • bida
  • bid
  • Bid
  • LOC100355991
  • LOC100730546
Reactivity
Human, Mouse (Murine), Rat (Rattus)
319
173
73
9
5
3
2
Host
Rabbit
294
95
20
9
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
346
185
124
84
52
41
32
21
13
11
11
6
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4284618
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4284613 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN4284615 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 4
1 ABIN957167 IHC (fro) IHC (p) IP WB Rabbit AA 1-55 Log in to see Polyclonal
1 ABIN957166 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN1077863 ICC IHC IP WB Rabbit IgG AA 1-196 Log in to see Polyclonal
1 ABIN1584098 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4284617 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN4284614 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN4284612 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN537391 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 3

Top referenced anti-BID antibody for Immunoprecipitation

Similar anti-BID Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 12 Antibodies 57 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 4 Antibodies
Immunofluorescence (IF) 3 Antibodies 7 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 27 Antibodies
Immunohistochemistry (IHC) 25 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 45 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 7 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(319), (173), (73), (9), (5), (3), (2)
Host Rabbit
(294), (95), (20), (9)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(346), (185), (124), (84), (52), (41), (32), (21), (13), (11), (11), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BID Antibody

Target Details BID Application Details Handling ProductDetails: References for anti-BID antibody (ABIN4284618) Images
Specificity Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by capspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites, generally 11-15 kDa C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.
Purification Unpurified
Immunogen A synthetic peptide corresponding to amino acids 28-50 (CSDNSFRRELDALGHELPVLAPQ) of human Bid (also known as Bid, isoform 2) was used as immunogen, GenBank no. NP_001187.1. Human Bid is a 195 amino acid protein.

Target Details BID

Product Details anti-BID Antibody Application Details Handling ProductDetails: References for anti-BID antibody (ABIN4284618) Images back to top
Antigen
Alternative Name BID (BID Antibody Abstract)
Background Gene Symbol: BID
Gene ID 637
NCBI Accession NP_001187
Research Area Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BID Antibody Target Details BID Handling ProductDetails: References for anti-BID antibody (ABIN4284618) Images back to top
Application Notes Western Blot 1:1000-1:2000, Immunohistochemistry, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details ProductDetails: References for anti-BID antibody (ABIN4284618) Images back to top
Format Liquid
Buffer Whole antisera
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-BID antibody (ABIN4284618)

Product Details anti-BID Antibody Target Details BID Application Details Handling Images back to top
Product cited in:

Krajewska, Rosenthal, Mikolajczyk, Stennicke, Wiesenthal, Mai, Naito, Salvesen, Reed, Fiskum, Krajewski: "Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation." in: Experimental neurology, Vol. 189, Issue 2, pp. 261-79, 2004 Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling ProductDetails: References for anti-BID antibody (ABIN4284618) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN4284618) Immunohistochemistry-Paraffin: Bid Antibody [ABIN42846188] - Staining of Mouse Embryo...
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN4284618) Western Blot: Bid Antibody [ABIN42846188] - Analysis of Bid using this antibody. this...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (ABIN4284618) Immunohistochemistry-Paraffin: Bid Antibody [ABIN42846188] - Staining of Human tissue...