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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||13 Antibodies||48 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||23 Antibodies||23 Antibodies|
|Immunohistochemistry (IHC)||22 Antibodies||45 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||21 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||33 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Antigen||BH3 Interacting Domain Death Agonist (BID) Antibodies|
|Epitope||AA 179-195, C-Term Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This BID antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-BID AntibodyTarget Details BID Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for BH3-interacting domain death agonist(Bid) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: BH3 interacting domain death agonist
Protein Name: BH3-interacting domain death agonist
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid(179-195aa NQNLFSYVRNLVRNEMD), different from the related rat sequence by one amino acid.|
Target Details BIDProduct Details anti-BID Antibody Application Details Handling Images back to top
|Alternative Name||Bid (BID Antibody Abstract)|
|Background||BID(BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al.(1998) mapped the human BID gene to 22q11. Luo et al.(1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.|
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-BID Antibody Target Details BID Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Predicted Species: Rat, The detection limit for BID is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-BID Antibody Target Details BID Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-BID Antibody Target Details BID Application Details Handling back to top