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anti-Rat (Rattus) BID antibody for Western Blotting

Recommended BID Antibody (supplied by: Log in to see )

Antigen
BH3 Interacting Domain Death Agonist (BID) Antibodies
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • DKFZp469E066
  • FP497
  • fp497
  • si:ch211-238n5.6
  • xbid
Epitope
AA 179-195, C-Term
50
42
34
17
17
14
13
11
10
8
6
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
308
142
62
4
2
2
1
Host
Rabbit
282
91
21
10
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
21
19
14
9
7
7
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
317
188
121
71
42
34
30
20
14
13
12
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN3042950
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12917738 ABIN3042321 ICC IHC (p) WB Rabbit IgG AA 1-195 Log in to see Polyclonal
0.12917738 ABIN1871288 IHC WB Rabbit IgG Log in to see Polyclonal
0.12917738 ABIN2163012 IHC ELISA WB Rabbit IgG Ser78 Log in to see Polyclonal
0.12917738 ABIN3020688 IHC WB Rabbit IgG Log in to see Polyclonal
0.12917738 ABIN1858137 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 1-196 Log in to see
0.12917738 ABIN4284618 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
0.12917738 ABIN1077863 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-196 Log in to see Polyclonal
0.12917738 ABIN4284615 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 3
0.12917738 ABIN408125 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
0.12917738 ABIN2421273 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12917738 ABIN2403432 IHC WB Rabbit IgG Log in to see Polyclonal
0.12917738 ABIN2427849 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011819944 ABIN272120 IHC (p) WB Rabbit Log in to see Polyclonal
0.0011819944 ABIN756757 IF (p) IHC (p) WB Rabbit IgG pSer65 Log in to see Polyclonal
0.0011819944 ABIN756759 IHC (p) WB Biotin Rabbit IgG pSer65 Log in to see Polyclonal
0.0011819944 ABIN756766 IHC (p) WB HRP Rabbit IgG pSer65 Log in to see Polyclonal
0.0011819944 ABIN1584098 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011819944 ABIN2957879 IC IF IHC WB Rabbit Log in to see Polyclonal
0.0011819944 ABIN4284613 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 1
0.0011819944 ABIN1715128 IF (p) IHC (p) WB Rabbit IgG pSer78 Log in to see Polyclonal

Top referenced anti-BID antibody for Western Blotting

Similar anti-BID Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 13 Antibodies 48 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 4 Antibodies
Immunofluorescence (IF) 3 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 33 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 6 Antibodies

General

Antigen BH3 Interacting Domain Death Agonist (BID) Antibodies
Epitope AA 179-195, C-Term
(50), (42), (34), (17), (17), (14), (13), (11), (10), (8), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(308), (142), (62), (4), (2), (2), (1)
Host Rabbit
(282), (91), (21), (10)
Clonality
Polyclonal
Conjugate This BID antibody is un-conjugated
(21), (19), (14), (9), (7), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(317), (188), (121), (71), (42), (34), (30), (20), (14), (13), (12), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-BID Antibody

Target Details BID Application Details Handling Images
Sequence NQNLFSYVRN LVRNEMD
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for BH3-interacting domain death agonist(Bid) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: BH3 interacting domain death agonist
Protein Name: BH3-interacting domain death agonist
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid(179-195aa NQNLFSYVRNLVRNEMD), different from the related rat sequence by one amino acid.
Isotype IgG

Target Details BID

Product Details anti-BID Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bid (BID Antibody Abstract)
Background BID(BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al.(1998) mapped the human BID gene to 22q11. Luo et al.(1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
UniProt P70444
Research Area Cancer, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BID Antibody Target Details BID Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Predicted Species: Rat, The detection limit for BID is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-BID Antibody Target Details BID Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-BID Antibody Target Details BID Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 179-195) (ABIN3042950) Anti-Bid antibody, Western blotting All lanes: Anti Bid at 0.5ug/ml WB: Mouse Brain T...
Immunohistochemistry (IHC) image for anti-BID antibody (BH3 Interacting Domain Death Agonist) (AA 179-195) (ABIN3042950) Anti-Bid antibody, IHC(P) IHC(P): Rat Spleen Tissue