You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human Caspase 10 antibody for Enzyme Immunoassay

Recommended Caspase 10 Antibody (supplied by: Log in to see )

Antigen
Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10) Antibodies
  • alps2
  • ALPS2
  • caspace-10
  • caspase-10
  • FLICE2
  • flice2
  • MCH4
  • mch4
  • xCaspace-10
  • xCaspase-10
Epitope
C-Term
27
8
5
5
5
4
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
138
20
18
1
Host
Rabbit
123
9
3
2
1
Clonality
Polyclonal
Conjugate
This Caspase 10 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
109
45
32
21
19
11
8
7
3
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN499545
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN1449660 EIA IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-Caspase 10 antibody for Enzyme Immunoassay

  • Human Polyclonal Caspase 10 Primary Antibody for EIA, WB - ABIN499545 (4 Publications): Fernandes-Alnemri, Armstrong, Krebs, Srinivasula, Wang, Bullrich, Fritz, Trapani, Tomaselli, Litwack, Alnemri: In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. in Proceedings of the National Academy of Sciences of the United States of America 1996 (PubMed)

Similar anti-Caspase 10 Antibodies

Application / Reactivity Human
ELISA 45 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 23 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 112 Antibodies

General

Antigen Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10) Antibodies
Epitope C-Term
(27), (8), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(138), (20), (18), (1)
Host Rabbit
(123), (9), (3), (2), (1)
Clonality
Polyclonal
Conjugate This Caspase 10 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(109), (45), (32), (21), (19), (11), (8), (7), (3), (2), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-Caspase 10 Antibody

Target Details Caspase 10 Application Details Handling References for anti-Caspase 10 Antibody (ABIN499545) Images
Specificity Caspase-10 antibody was raised against a peptide corresponding to amino acids 505 to 521 of human FLICE2. Antibody only recognizes FLICE2 form of caspase-10.
Purification DEAE
Immunogen Human Caspase-10 (C-Terminus) Peptide
Isotype IgG

Target Details Caspase 10

Product Details anti-Caspase 10 Antibody Application Details Handling References for anti-Caspase 10 Antibody (ABIN499545) Images back to top
Antigen
Alternative Name Caspase-10 (CASP10 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch41,2 and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis1. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.Synonyms: Apoptotic protease Mch-4, CASP-10, CASP10, FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2, ICE-like apoptotic protease 4, MCH4
Gene ID 843
UniProt Q92851
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Caspase 10 Antibody Target Details Caspase 10 Handling References for anti-Caspase 10 Antibody (ABIN499545) Images back to top
Application Notes ELISA. Western Blot: Caspase-10 antibody can be used for detection of caspase-10 at 1/500 to1/1000 dilution. A 59 kDa band can be detected.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 10 Antibody Target Details Caspase 10 Application Details References for anti-Caspase 10 Antibody (ABIN499545) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Caspase 10 Antibody (ABIN499545)

Product Details anti-Caspase 10 Antibody Target Details Caspase 10 Application Details Handling Images back to top
Background publications

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997

Vincenz, Dixit: "Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling." in: The Journal of biological chemistry, Vol. 272, Issue 10, pp. 6578-83, 1997

Fernandes-Alnemri, Armstrong, Krebs, Srinivasula, Wang, Bullrich, Fritz, Trapani, Tomaselli, Litwack, Alnemri: "In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 15, pp. 7464-9, 1996

Chavrier, Vingron, Sander, Simons, Zerial: "Molecular cloning of YPT1/SEC4-related cDNAs from an epithelial cell line." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6578-85, 1991

Images

Product Details anti-Caspase 10 Antibody Target Details Caspase 10 Application Details Handling References for anti-Caspase 10 Antibody (ABIN499545) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 10 antibody (Caspase 10, Apoptosis-Related Cysteine Peptidase) (C-Term) (ABIN499545) Western blot analysis of caspase-10 in HeLa (H), Jurkat (J), A431 (A), K562 (K) whole...