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anti-Human Caspase 2 antibody for BioImaging

Recommended Caspase 2 Antibody (supplied by: Log in to see )

Antigen
Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antibodies
  • CASP-2
  • Caspase-2
  • ICH-1
  • ICH-1L/1S
  • ICH1
  • ICH1L1S
  • NEDD-2
  • Nedd2
  • NEDD2
  • PPP1R57
  • xCaspase-2
Epitope
Short Isoform
27
26
15
12
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
202
64
46
6
2
2
Host
Mouse
160
52
11
Clonality
Monoclonal
Conjugate
This Caspase 2 antibody is un-conjugated
6
6
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
BioImaging (BI), Western Blotting (WB)
178
102
41
30
13
12
11
10
9
7
4
4
1
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Available images

Catalog No. ABIN967316
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Application / Reactivity Human
BioImaging (BI) 1 Antibodies
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Flow Cytometry (FACS) 10 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 41 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 162 Antibodies

General

Antigen Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antibodies
Epitope Short Isoform
(27), (26), (15), (12), (6), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(202), (64), (46), (6), (2), (2)
Host Mouse
(160), (52), (11)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 2 antibody is un-conjugated
(6), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application BioImaging (BI), Western Blotting (WB)
(178), (102), (41), (30), (13), (12), (11), (10), (9), (7), (4), (4), (1)
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Product Details anti-Caspase 2 Antibody

Target Details Caspase 2 Application Details Handling Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Triton is a trademark of the Dow Chemical Company.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Caspase-2 (short)
Clone G310
Isotype IgG1

Target Details Caspase 2

Product Details anti-Caspase 2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Caspase-2 (CASP2 Antibody Abstract)
Background Caspase-2 (Ich-1) belongs to the ICE/CED-3 cell death gene family of cysteine proteases. CED-3 is a C. elegans death gene that is required for normal programmed cell death during development. ICE (interleukin-1ß-converting enzyme) is the mammalian homolog of CED-3 and is activated when cells become apoptotic. Ich1 mRNA is alternatively spliced into two different forms, Ich-1 long (Ich-1L) and Ich-1 short (Ich-1S). Ich-1L is a 48 kDa protein that has about 50% amino acid sequence homology to both CED-3 and ICE. Ich-1S (35 kDa) is a truncated version of Ich-1L. Ich-1L and Ich-1S have been found to have opposite effects on cell death in vitro. Overexpression of Ich-1L in Rat1 rat fibroblast cells induced programmed cell death, whereas overexpression of Ich-1S suppressed Rat-1 cell death induced by serum starvation. These results suggest that Ich-1 may play a role in both the positive and negative regulation of programmed cell death.
The G310-1248 clone was made using recombinant full-length human caspase-2 (short) [Ich-1S] as the immunogen and can recognize both the short and long forms of human caspase-2.
Research Area Apoptosis/Necrosis, Cancer, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Handling Images back to top
Application Notes Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store both the antibody and Jurkat control lysate undiluted at -20°C.

Images

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 2 antibody (Caspase 2, Apoptosis-Related Cysteine Peptidase) (Short Isoform) (ABIN967316) Western blot analysis of Caspase-2. Lysate from Jurkat cells were probed with the Cas...
Immunofluorescence (IF) image for anti-Caspase 2 antibody (Caspase 2, Apoptosis-Related Cysteine Peptidase) (Short Isoform) (ABIN967316) Immunofluorescent staining of U-2 OS (ATCC HTB-96) cells. Cells were seeded in a 96 w...