anti-Mouse (Murine) Caspase 2 antibody for Enzyme Immunoassay

Recommended Caspase 2 Antibody (supplied by: Log in to see )

Antigen
Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antibodies
  • xCaspase-2
  • Caspase-2
  • ICH-1
  • Nedd2
  • CASP-2
  • ICH1
  • NEDD-2
  • NEDD2
  • PPP1R57
  • ICH-1L/1S
  • ICH1L1S
  • caspase-2-like
  • caspase-2
  • caspase 2
  • caspase 2, apoptosis-related cysteine peptidase
  • LOC100050611
  • xCaspase-2
  • CpipJ_CPIJ008254
  • Casp2
  • LOC100101590
  • CASP2
  • LOC100521118
Epitope
N-Term
27
20
14
9
7
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
210
89
68
10
8
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
155
51
13
Clonality
Polyclonal
Conjugate
This Caspase 2 antibody is un-conjugated
6
6
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
179
98
47
35
17
14
13
12
12
4
4
3
2
1
Supplier
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Catalog No. ABIN5556699
$ 411.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5554658 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal

Similar anti-Caspase 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 17 Antibodies 24 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 6 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 14 Antibodies
Immunofluorescence (IF) 2 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 15 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 49 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies

General

Antigen Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antibodies
Epitope N-Term
(27), (20), (14), (9), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(210), (89), (68), (10), (8), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(155), (51), (13)
Clonality
Polyclonal
Conjugate This Caspase 2 antibody is un-conjugated
(6), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(179), (98), (47), (35), (17), (14), (13), (12), (12), (4), (4), (3), (2), (1)
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Product Details anti-Caspase 2 Antibody

Target Details Caspase 2 Application Details Handling Images
Sequence M-A-A-S-S-G-R-S-Q-S-S-L-H-R-K-G-L-C-KLH
Specificity This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. Antiserum was purified against the immunizing peptide. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94 % homology. There is no significant homology to the human form.
Characteristics Concentration: 0.67 mg/mL (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography.
Immunogen A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.

Target Details Caspase 2

Product Details anti-Caspase 2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Caspase-2 (CASP2 Antibody Abstract)
Background Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer and other diseases. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse. Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis. The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B. Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7. Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue. Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2. These forms show tissue specificity and function either as inducers or suppressors of apoptosis. Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.Synonyms: CASP-2, CASP2, ICH-1 protease, ICH1, NEDD2, Neural precursor cell expressed developmentally down-regulated protein 2
Gene ID 12366
NCBI Accession NP_031636
UniProt P29594
Research Area Apoptosis/Necrosis, Autophagy, Cancer
Pathways Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Handling Images back to top
Application Notes This product was assayed against 1.0 g of immunizing peptide in a standard sandwichELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as asubstrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 10,000 issuggested for this product. This affinity purified polyclonal antibody was tested by immunoblotting and was found tobe reactive with 48 kDa mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by
Restrictions For Research Use only

Handling

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details Images back to top
Concentration 0.67 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C.