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anti-Rat (Rattus) Caspase 2 antibody for Enzyme Immunoassay

Recommended Caspase 2 Antibody (supplied by: Log in to see )

Antigen
Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antibodies
  • CASP-2
  • Caspase-2
  • ICH-1
  • ICH-1L/1S
  • ICH1
  • ICH1L1S
  • NEDD-2
  • Nedd2
  • NEDD2
  • PPP1R57
  • xCaspase-2
Epitope
N-Term
23
15
12
9
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
179
60
43
6
2
2
Host
Rabbit
154
33
11
Clonality
Polyclonal
Conjugate
This Caspase 2 antibody is un-conjugated
5
5
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
154
81
40
29
11
11
10
10
9
7
4
3
1
1
Supplier
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Catalog No. ABIN118007
$ 500.50
Plus shipping costs $45.00

Top referenced anti-Caspase 2 antibody for Enzyme Immunoassay

Similar anti-Caspase 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 12 Antibodies 15 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 1 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 12 Antibodies
Immunoprecipitation (IP) 4 Antibodies 4 Antibodies
Western Blotting (WB) 30 Antibodies
Immunocytochemistry (ICC) 6 Antibodies

General

Antigen Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) Antibodies
Epitope N-Term
(23), (15), (12), (9), (6), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(179), (60), (43), (6), (2), (2)
Host Rabbit
(154), (33), (11)
Clonality
Polyclonal
Conjugate This Caspase 2 antibody is un-conjugated
(5), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(154), (81), (40), (29), (11), (11), (10), (10), (9), (7), (4), (3), (1), (1)
Pubmed 4 references available
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Product Details anti-Caspase 2 Antibody

Target Details Caspase 2 Application Details Handling References for anti-Caspase 2 Antibody (ABIN118007) Images
Sequence M-A-A-S-S-G-R-S-Q-S-S-L-H-R-K-G-L-C-KLH
Specificity This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. Antiserum was purified against the immunizing peptide. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94 % homology. There is no significant homology to the human form.
Purification Immunoaffinity chromatography.
Immunogen A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.

Target Details Caspase 2

Product Details anti-Caspase 2 Antibody Application Details Handling References for anti-Caspase 2 Antibody (ABIN118007) Images back to top
Antigen
Alternative Name Caspase-2 (CASP2 Antibody Abstract)
Background Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer and other diseases. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse. Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis. The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B. Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7. Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue. Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2. These forms show tissue specificity and function either as inducers or suppressors of apoptosis. Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.Synonyms: CASP-2, CASP2, ICH-1 protease, ICH1, NEDD2, Neural precursor cell expressed developmentally down-regulated protein 2
Gene ID 12366
NCBI Accession NP_031636
UniProt P29594
Research Area Apoptosis/Necrosis, Cancer, Autophagy
Pathways Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Handling References for anti-Caspase 2 Antibody (ABIN118007) Images back to top
Application Notes This product was assayed against 1.0 g of immunizing peptide in a standard sandwichELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as asubstrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 10,000 issuggested for this product. This affinity purified polyclonal antibody was tested by immunoblotting and was found tobe reactive with 48 kDa mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by
Restrictions For Research Use only

Handling

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details References for anti-Caspase 2 Antibody (ABIN118007) Images back to top
Concentration 0.67 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C.

References for anti-Caspase 2 Antibody (ABIN118007)

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details Handling Images back to top
Background publications

Bergeron, Perez, Macdonald, Shi, Sun, Jurisicova, Varmuza, Latham, Flaws, Salter, Hara, Moskowitz, Li, Greenberg, Tilly, Yuan: "Defects in regulation of apoptosis in caspase-2-deficient mice." in: Genes & development, Vol. 12, Issue 9, pp. 1304-14, 1998

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997

Li, Bergeron, Cryns, Pasternack, Zhu, Shi, Greenberg, Yuan: "Activation of caspase-2 in apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 34, pp. 21010-7, 1997

Wang, Miura, Bergeron, Zhu, Yuan: "Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death." in: Cell, Vol. 78, Issue 5, pp. 739-50, 1994

Images

Product Details anti-Caspase 2 Antibody Target Details Caspase 2 Application Details Handling References for anti-Caspase 2 Antibody (ABIN118007) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 2 antibody (Caspase 2, Apoptosis-Related Cysteine Peptidase) (N-Term) (ABIN118007) Immunoblotting. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution ...