anti-Mouse (Murine) CFLAR antibody for Immunoprecipitation

Recommended CFLAR Antibody (supplied by: Log in to see )

Antigen
CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
  • Clarp1
  • cflar
  • CFLAR
  • FLIP
  • CASH
  • CASP8AP1
  • CLARP
  • Casper
  • FLAME
  • FLAME-1
  • FLAME1
  • I-FLICE
  • MRIT
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • 2310024N18Rik
  • A430105C05Rik
  • Cash
  • ENSMUSG00000072980
  • Flip
  • Gm9845
  • c-Flip
  • CASP8 and FADD-like apoptosis regulator a
  • CASP8 and FADD-like apoptosis regulator
  • cellular FLICE-like inhibitory protein
  • cflara
  • CFLAR
  • cflar
  • Cflar
  • C-FLIP
Epitope
AA 1-480
41
37
24
22
17
15
15
14
10
7
6
6
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
281
135
90
6
2
2
1
1
Host
Rat
161
114
22
Clonality
Monoclonal
Conjugate
This CFLAR antibody is un-conjugated
11
8
7
6
6
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
256
120
103
43
39
30
29
26
20
8
4
2
2
1
1
1
1
Options
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Catalog No. ABIN1169019
$ 456.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN601341 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2962777 IP WB Rat IgG2a AA 1-480 Log in to see Dave-2
1 ABIN1858388 ICC IHC IP WB Rabbit IgG AA 210-460 Log in to see Polyclonal
1 ABIN2619215 IP WB Rat IgG2a AA 1-200, AA 1-480 Log in to see Dave-2
1 ABIN1492976 IHC (p) IP WB Sepharose Rabbit C-Term Log in to see Polyclonal
1 ABIN1492966 IHC (p) IP WB Magnetic Particles Rabbit C-Term Log in to see Polyclonal

General

Antigen CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
Epitope AA 1-480
(41), (37), (24), (22), (17), (15), (15), (14), (10), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(281), (135), (90), (6), (2), (2), (1), (1)
Host Rat
(161), (114), (22)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CFLAR antibody is un-conjugated
(11), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(256), (120), (103), (43), (39), (30), (29), (26), (20), (8), (4), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CFLAR Antibody

Target Details CFLAR Application Details Handling References for anti-CFLAR antibody (ABIN1169019) Images
Specificity Recognizes an epitope (aa 1-200) present in both short (FLIPS) and long (FLIPL) splice variants of human and mouse FLIP.
Cross-Reactivity Human, Mouse (Murine)
Immunogen Recombinant human FLIP (aa 1-480).
Clone Dave-2
Isotype IgG2a

Target Details CFLAR

Product Details anti-CFLAR Antibody Application Details Handling References for anti-CFLAR antibody (ABIN1169019) Images back to top
Antigen
Alternative Name FLIP (CFLAR Antibody Abstract)
Background FLIP is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity. FLIP is highly expressed in skeletal muscle, pancreas, heart, kidney, placenta and peripheral blood leukocytes.
UniProt O15519
Research Area Cancer, Apoptosis/Necrosis, Signaling
Pathways Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development

Application Details

Product Details anti-CFLAR Antibody Target Details CFLAR Handling References for anti-CFLAR antibody (ABIN1169019) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details References for anti-CFLAR antibody (ABIN1169019) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months

References for anti-CFLAR antibody (ABIN1169019)

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling Images back to top
Product cited in:

Lens, Kataoka, Fortner, Tinel, Ferrero, MacDonald, Hahne, Beermann, Attinger, Orbea, Budd, Tschopp: "The caspase 8 inhibitor c-FLIP(L) modulates T-cell receptor-induced proliferation but not activation-induced cell death of lymphocytes." in: Molecular and cellular biology, Vol. 22, Issue 15, pp. 5419-33, 2002

Gómez-Angelats, Cidlowski: "Protein kinase C regulates FADD recruitment and death-inducing signaling complex formation in Fas/CD95-induced apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 44944-52, 2001

Micheau, Lens, Gaide, Alevizopoulos, Tschopp: "NF-kappaB signals induce the expression of c-FLIP." in: Molecular and cellular biology, Vol. 21, Issue 16, pp. 5299-305, 2001

Djerbi, Darreh-Shori, Zhivotovsky, Grandien: "Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different flip isoforms." in: Scandinavian journal of immunology, Vol. 54, Issue 1-2, pp. 180-9, 2001

Rescigno, Piguet, Valzasina, Lens, Zubler, French, Kindler, Tschopp, Ricciardi-Castagnoli et al.: "Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate ..." in: The Journal of experimental medicine, Vol. 192, Issue 11, pp. 1661-8, 2000

Images

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Supplier Images
Western Blotting (WB) image for anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 1-480) antibody (ABIN1169019) Detection of human and mouse FLIP in 293T cells transfected with a human (lane 2) or ...