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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||26 Antibodies||59 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies||7 Antibodies|
|Flow Cytometry (FACS)||4 Antibodies||13 Antibodies|
|Immunocytochemistry (ICC)||13 Antibodies||23 Antibodies|
|Immunofluorescence (IF)||7 Antibodies||22 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||12 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||10 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|SDS-PAGE (SDS)||1 Antibodies|
|Western Blotting (WB)||57 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Antigen||CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies|
|Epitope||AA 1-480 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This CFLAR antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-CFLAR AntibodyTarget Details CFLAR Application Details Handling References for anti-CFLAR Antibody (ABIN1169019) Images
|Specificity||Recognizes an epitope (aa 1-200) present in both short (FLIPS) and long (FLIPL) splice variants of human and mouse FLIP.|
|Cross-Reactivity||Human, Mouse (Murine)|
|Immunogen||Recombinant human FLIP (aa 1-480).|
Target Details CFLARProduct Details anti-CFLAR Antibody Application Details Handling References for anti-CFLAR Antibody (ABIN1169019) Images back to top
|Alternative Name||FLIP (CFLAR Antibody Abstract)|
|Background||FLIP is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity. FLIP is highly expressed in skeletal muscle, pancreas, heart, kidney, placenta and peripheral blood leukocytes.|
|Research Area||Cancer, Apoptosis/Necrosis, Signaling|
Application DetailsProduct Details anti-CFLAR Antibody Target Details CFLAR Handling References for anti-CFLAR Antibody (ABIN1169019) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CFLAR Antibody Target Details CFLAR Application Details References for anti-CFLAR Antibody (ABIN1169019) Images back to top
|Buffer||Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
|Expiry Date||12 months|
References for anti-CFLAR Antibody (ABIN1169019)Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling Images back to top
|Product cited in:||
Lens, Kataoka, Fortner et al.: "The caspase 8 inhibitor c-FLIP(L) modulates T-cell receptor-induced proliferation but not activation-induced cell death of lymphocytes." in: Molecular and cellular biology, Vol. 22, Issue 15, pp. 5419-33, 2002 (PubMed).
Gómez-Angelats, Cidlowski: "Protein kinase C regulates FADD recruitment and death-inducing signaling complex formation in Fas/CD95-induced apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 44944-52, 2001 (PubMed).
Micheau, Lens, Gaide et al.: "NF-kappaB signals induce the expression of c-FLIP." in: Molecular and cellular biology, Vol. 21, Issue 16, pp. 5299-305, 2001 (PubMed).
Djerbi, Darreh-Shori, Zhivotovsky et al.: "Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different flip isoforms." in: Scandinavian journal of immunology, Vol. 54, Issue 1-2, pp. 180-9, 2001 (PubMed).
Rescigno, Piguet, Valzasina et al.: "Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate ..." in: The Journal of experimental medicine, Vol. 192, Issue 11, pp. 1661-8, 2000 (PubMed).
ImagesProduct Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling References for anti-CFLAR Antibody (ABIN1169019) back to top