anti-Mouse (Murine) CFLAR antibody for Immunoprecipitation

Recommended CFLAR Antibody (supplied by: Log in to see )

Antigen
CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
  • Clarp1
  • cflar
  • CFLAR
  • FLIP
  • CASH
  • CASP8AP1
  • CLARP
  • Casper
  • FLAME
  • FLAME-1
  • FLAME1
  • I-FLICE
  • MRIT
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • 2310024N18Rik
  • A430105C05Rik
  • Cash
  • ENSMUSG00000072980
  • Flip
  • Gm9845
  • c-Flip
  • CASP8 and FADD-like apoptosis regulator a
  • CASP8 and FADD-like apoptosis regulator
  • cellular FLICE-like inhibitory protein
  • cflara
  • CFLAR
  • cflar
  • Cflar
  • C-FLIP
Epitope
AA 1-480
37
24
22
16
15
15
14
10
10
7
6
6
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
249
119
91
6
2
2
1
1
Host
Rat
160
83
22
Clonality
Monoclonal
Conjugate
This CFLAR antibody is un-conjugated
9
6
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
230
92
75
43
39
30
26
19
14
8
4
2
2
1
1
1
1
Options
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Catalog No. ABIN1169019
$ 456.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1858388 ICC IHC IP WB Rabbit IgG AA 210-460 Log in to see Polyclonal
1 ABIN2962777 IP WB Rat IgG2a AA 1-480 Log in to see Dave-2
1 ABIN601341 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2619215 IP WB Rat IgG2a AA 1-200, AA 1-480 Log in to see Dave-2
1 ABIN1492966 IHC (p) IP WB Magnetic Particles Rabbit C-Term Log in to see Polyclonal
1 ABIN1492976 IHC (p) IP WB Sepharose Rabbit C-Term Log in to see Polyclonal

Top referenced anti-CFLAR antibody for Immunoprecipitation

  • Human Monoclonal CFLAR Primary Antibody for IP, WB - ABIN1169019 (5 Publications): Rescigno, Piguet, Valzasina, Lens, Zubler, French, Kindler, Tschopp, Ricciardi-Castagnoli et al.: Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate ... in The Journal of experimental medicine 2000 (PubMed)

Similar anti-CFLAR Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies 1 Antibodies
ELISA 50 Antibodies 85 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 7 Antibodies
Flow Cytometry (FACS) 8 Antibodies 14 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 7 Antibodies
SDS-PAGE (SDS) 1 Antibodies
Western Blotting (WB) 94 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
Epitope AA 1-480
(37), (24), (22), (16), (15), (15), (14), (10), (10), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(249), (119), (91), (6), (2), (2), (1), (1)
Host Rat
(160), (83), (22)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CFLAR antibody is un-conjugated
(9), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(230), (92), (75), (43), (39), (30), (26), (19), (14), (8), (4), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CFLAR Antibody

Target Details CFLAR Application Details Handling ProductDetails: References for anti-CFLAR antibody (ABIN1169019) Images
Specificity Recognizes an epitope (aa 1-200) present in both short (FLIPS) and long (FLIPL) splice variants of human and mouse FLIP.
Cross-Reactivity Human, Mouse (Murine)
Immunogen Recombinant human FLIP (aa 1-480).
Clone Dave-2
Isotype IgG2a

Target Details CFLAR

Product Details anti-CFLAR Antibody Application Details Handling ProductDetails: References for anti-CFLAR antibody (ABIN1169019) Images back to top
Antigen
Alternative Name FLIP (CFLAR Antibody Abstract)
Background FLIP is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity. FLIP is highly expressed in skeletal muscle, pancreas, heart, kidney, placenta and peripheral blood leukocytes.
UniProt O15519
Research Area Cancer, Apoptosis/Necrosis, Signaling
Pathways Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development

Application Details

Product Details anti-CFLAR Antibody Target Details CFLAR Handling ProductDetails: References for anti-CFLAR antibody (ABIN1169019) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details ProductDetails: References for anti-CFLAR antibody (ABIN1169019) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months

ProductDetails: References for anti-CFLAR antibody (ABIN1169019)

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling Images back to top
Product cited in:

Lens, Kataoka, Fortner, Tinel, Ferrero, MacDonald, Hahne, Beermann, Attinger, Orbea, Budd, Tschopp: "The caspase 8 inhibitor c-FLIP(L) modulates T-cell receptor-induced proliferation but not activation-induced cell death of lymphocytes." in: Molecular and cellular biology, Vol. 22, Issue 15, pp. 5419-33, 2002

Gómez-Angelats, Cidlowski: "Protein kinase C regulates FADD recruitment and death-inducing signaling complex formation in Fas/CD95-induced apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 44944-52, 2001

Micheau, Lens, Gaide, Alevizopoulos, Tschopp: "NF-kappaB signals induce the expression of c-FLIP." in: Molecular and cellular biology, Vol. 21, Issue 16, pp. 5299-305, 2001

Djerbi, Darreh-Shori, Zhivotovsky, Grandien: "Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different flip isoforms." in: Scandinavian journal of immunology, Vol. 54, Issue 1-2, pp. 180-9, 2001

Rescigno, Piguet, Valzasina, Lens, Zubler, French, Kindler, Tschopp, Ricciardi-Castagnoli et al.: "Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate ..." in: The Journal of experimental medicine, Vol. 192, Issue 11, pp. 1661-8, 2000

Images

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling ProductDetails: References for anti-CFLAR antibody (ABIN1169019) back to top
Supplier Images
Western Blotting (WB) image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (AA 1-480) (ABIN1169019) Detection of human and mouse FLIP in 293T cells transfected with a human (lane 2) or ...