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anti-Rat (Rattus) CFLAR antibody for Immunofluorescence

Recommended CFLAR Antibody (supplied by: Log in to see )

Antigen
CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
  • Clarp1
  • cflar
  • CFLAR
  • FLIP
  • CASH
  • CASP8AP1
  • CLARP
  • Casper
  • FLAME
  • FLAME-1
  • FLAME1
  • I-FLICE
  • MRIT
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • 2310024N18Rik
  • A430105C05Rik
  • Cash
  • ENSMUSG00000072980
  • Flip
  • Gm9845
  • c-Flip
  • CASP8 and FADD-like apoptosis regulator a
  • CASP8 and FADD-like apoptosis regulator
  • cellular FLICE-like inhibitory protein
  • cflara
  • CFLAR
  • cflar
  • Cflar
  • C-FLIP
Epitope
C-Term
36
24
23
16
15
15
14
10
10
7
6
6
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
249
119
90
6
2
2
1
1
Host
Rabbit
159
83
23
Clonality
Polyclonal
Conjugate
This CFLAR antibody is un-conjugated
9
6
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
230
92
75
43
39
29
26
20
14
7
4
2
2
1
1
1
1
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Catalog No. ABIN499848
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.087689 ABIN499846 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 4
11.087689 ABIN4312111 ELISA ICC IF WB Rabbit C-Term Log in to see Polyclonal
11.087689 ABIN4312112 ELISA FACS ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN449670 ELISA ICC IF SDS WB Mouse IgG3 kappa AA 1-376 Log in to see 5D8
1 ABIN222911 IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1031381 FACS IF IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4965081 IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN241636 IF WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1888196 FACS IF IHC ELISA WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-CFLAR antibody for Immunofluorescence

Similar anti-CFLAR Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 37 Antibodies 50 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 4 Antibodies
Flow Cytometry (FACS) 3 Antibodies 8 Antibodies
Immunocytochemistry (ICC) 11 Antibodies 18 Antibodies
Immunofluorescence (IF) 10 Antibodies 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies 26 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 4 Antibodies
SDS-PAGE (SDS) 1 Antibodies
Western Blotting (WB) 65 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies

General

Antigen CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies
Epitope C-Term
(36), (24), (23), (16), (15), (15), (14), (10), (10), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(249), (119), (90), (6), (2), (2), (1), (1)
Host Rabbit
(159), (83), (23)
Clonality
Polyclonal
Conjugate This CFLAR antibody is un-conjugated
(9), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(230), (92), (75), (43), (39), (29), (26), (20), (14), (7), (4), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CFLAR Antibody

Target Details CFLAR Application Details Handling References for anti-CFLAR Antibody (ABIN499848) Images
Specificity FLIP antibody was raised against a peptide corresponding to amino acids near the C-terminus of human FLIPaFLIPl form. Antibody recognizes the FLIPa only.
Purification DEAE
Immunogen FLIP antibody was raised against a peptide corresponding to amino acids near the C- terminus of human FLIPaFLIPl form.
Isotype IgG

Target Details CFLAR

Product Details anti-CFLAR Antibody Application Details Handling References for anti-CFLAR Antibody (ABIN499848) Images back to top
Antigen
Alternative Name CFLAR / Casper / I-FLICE (CFLAR Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1,2. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively (8).Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP
Gene ID 8837
UniProt O15519
Pathways Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development

Application Details

Product Details anti-CFLAR Antibody Target Details CFLAR Handling References for anti-CFLAR Antibody (ABIN499848) Images back to top
Application Notes ELISA. Western Blot: 1/500 to 1/2000. A 55 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details References for anti-CFLAR Antibody (ABIN499848) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-CFLAR Antibody (ABIN499848)

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling Images back to top
Background publications

Thome, Schneider, Hofmann, Fickenscher, Meinl, Neipel, Mattmann, Burns, Bodmer, Schröter, Scaffidi, Krammer, Peter, Tschopp: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997

Irmler, Thome, Hahne, Schneider, Hofmann, Steiner, Bodmer, Schröter, Burns, Mattmann, Rimoldi, French, Tschopp: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997

Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997

Hu, Vincenz, Ni, Gentz, Dixit: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997

Images

Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling References for anti-CFLAR Antibody (ABIN499848) back to top
Supplier Images
Immunofluorescence (IF) image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (C-Term) (ABIN499848) Immunocytochemistry of FLIP in Jurkat cells with AP30342PU-N FLIP antibody at 10 μg/ml.
Western Blotting (WB) image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (C-Term) (ABIN499848) Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate...