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|Application / Reactivity||Human||Dog (Canine)||Horse (Equine)||Cow (Bovine)|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies|
|Cytometry by Time of Flight (CyTOF)||2 Antibodies||2 Antibodies||6 Antibodies|
|Western Blotting (WB)||60 Antibodies||82 Antibodies||233 Antibodies||18 Antibodies|
|Radioimmunoassay (RIA)||3 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Neutralization (Neut)||5 Antibodies|
|Intracellular Staining (ICS)||1 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||39 Antibodies||11 Antibodies||13 Antibodies||13 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||50 Antibodies||57 Antibodies|
|Antigen||Cytochrome C, Somatic (CYCS) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Horse (Equine), Human Alternatives|
|Conjugate||This Cytochrome C antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP)
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Product Details anti-Cytochrome C AntibodyTarget Details Cytochrome C Application Details Handling Images
|Specificity||This antibody detects Cytochrome c.|
|Characteristics||Synonyms: CYCS, CYC|
|Purification||Protein A chromatography|
|Immunogen||Equine cytochrome c|
|Clone||CTC03 (same as 2B5)|
Target Details Cytochrome CProduct Details anti-Cytochrome C Antibody Application Details Handling Images back to top
|Alternative Name||Cytochrome C (CYCS Antibody Abstract)|
|Background||Cytochrome c is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytochrome c has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome c is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome c functions in the activation of caspase-3, and ICE family molecule that is a key effector of apoptosis.Synonyms: CYC, CYCS|
|Research Area||Cancer, Apoptosis/Necrosis, Metabolism|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
ELISA. Flow Cytometry. Immunoprecipitation: Native verified, use Protein A, 2 g/mg protein lysate. Positive Control: HeLa cells. Not suitable for Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
|Buffer||10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|