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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|BioImaging (BI)||1 Antibodies||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||135 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||26 Antibodies|
|Immunofluorescence (IF)||52 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||49 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||15 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||120 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies|
|Intracellular Staining (ICS)||2 Antibodies|
|Neutralization (Neut)||8 Antibodies|
|Western Blotting (WB)||169 Antibodies|
|Antigen||Cytochrome C, Somatic (CYCS) Antibodies|
|Epitope||AA 2-105 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cytochrome C antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Cytochrome C AntibodyTarget Details Cytochrome C Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Cytochrome c(CYCS) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: cytochrome c, somatic
Protein Name: Cytochrome c
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.|
Target Details Cytochrome CProduct Details anti-Cytochrome C Antibody Application Details Handling Images back to top
|Alternative Name||CYCS (CYCS Antibody Abstract)|
|Background||CYCS is also known as CYC, HCS or THC4. This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Cytochrome C is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-Cytochrome C Antibody Target Details Cytochrome C Application Details Handling back to top