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anti-Rat (Rattus) DFFA antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • A330085O09Rik
  • DFF-45
  • DFF1
  • DFF35
  • Dff45
  • DFFA
  • ICAD
  • ICAD-L
  • ICAD-S
Reactivity
Human, Mouse (Murine), Rat (Rattus)
157
72
30
1
1
Host
Rabbit
184
32
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
178
73
40
24
23
20
11
11
5
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN272297
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089724595 ABIN726380 IF (p) IHC (p) Rabbit IgG AA 1-50 Log in to see Polyclonal
0.00089724595 ABIN726382 IHC (p) Biotin Rabbit IgG AA 1-50 Log in to see Polyclonal
0.00089724595 ABIN726389 IHC (p) HRP Rabbit IgG AA 1-50 Log in to see Polyclonal

Similar anti-DFFA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 8 Antibodies 16 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 3 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 8 Antibodies
Western Blotting (WB) 15 Antibodies 55 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Dot Blot (DB) 4 Antibodies

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(157), (72), (30), (1), (1)
Host Rabbit
(184), (32), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(178), (73), (40), (24), (23), (20), (11), (11), (5), (4), (4), (3), (2), (1), (1), (1)
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling Images
Specificity This antibody detects endogenous levels of ICAD protein. (region surrounding Gln177)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Purity > 95 % pure by SDS-PAGE

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling Images back to top
Antigen
Alternative Name DFFA / ICAD (DFFA Antibody Abstract)
Background The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a 112 kDa nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature 85 kDa apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the 45 kDa subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The 70 kDa nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
Molecular Weight approx. 36 kDa
Gene ID 13347
NCBI Accession NP_034174
UniProt O54786
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling Images back to top
Application Notes ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details Images back to top
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling back to top
Supplier Images
 image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (ABIN272297) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody
 image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (ABIN272297) anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody (Image 2)