anti-Rat (Rattus) DFFA antibody for Western Blotting

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • DNA fragmentation factor, alpha subunit
  • DFFA
  • dffa
  • Dffa
Epitope
N-Term
29
25
19
16
15
15
12
8
5
5
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
165
83
40
2
1
Host
Rabbit
189
36
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
190
70
46
35
32
27
17
13
6
4
4
3
2
2
1
Options
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Catalog No. ABIN2779329
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN1534436 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.3487515 ABIN2706028 WB Rabbit Center Log in to see Polyclonal
3.3487515 ABIN1872254 IHC IP WB Rabbit IgG cleaved Log in to see Polyclonal
3.3487515 ABIN3185120 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3487515 ABIN4305091 WB Rabbit IgG Center Log in to see Polyclonal
3.3487515 ABIN2736635 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN4305082 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN272297 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN222902 WB Rabbit IgG N-Term Log in to see Polyclonal 1
1 ABIN2889986 IHC (p) ELISA WB Rabbit IgG AA 151-200 Log in to see Polyclonal
1 ABIN331282 IP ELISA WB Mouse IgG2a, kappa Log in to see
1 ABIN2892223 IF IHC (p) WB Rabbit Gln177 Log in to see Polyclonal
1 ABIN1586053 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2740344 WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN2878569 IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN241627 WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1085306 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1085305 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN5005012 IP WB Mouse IgG2a kappa Log in to see AF24G5
1 ABIN2971897 WB Rabbit Center Log in to see Polyclonal

Top referenced anti-DFFA antibody for Western Blotting

Similar anti-DFFA Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Cow (Bovine) Guinea Pig Horse (Equine)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 2 Antibodies 1 Antibodies
Western Blotting (WB) 66 Antibodies 144 Antibodies 23 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 26 Antibodies 4 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies 25 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 19 Antibodies 36 Antibodies

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope N-Term
(29), (25), (19), (16), (15), (15), (12), (8), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(165), (83), (40), (2), (1)
Host Rabbit
(189), (36), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(190), (70), (46), (35), (32), (27), (17), (13), (6), (4), (4), (3), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling ProductDetails: References for anti-DFFA antibody (ABIN2779329) Images
Sequence MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL
Predicted Reactivity Cow: 92%, Dog: 83%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 83%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against DFFA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human DFFA

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling ProductDetails: References for anti-DFFA antibody (ABIN2779329) Images back to top
Antigen
Alternative Name DFFA (DFFA Antibody Abstract)
Background Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: DFF-45, DFF1, Ion ChannelAD, ICAD
Protein Interaction Partner: DFFB, UBC, TUBB, ATG3, ISOC1, BZW2, MAT2B, FERMT2, TUBA1A, CNBP, RPS6KA3, PPP5C, PFAS, MAT2A, EIF6, GAPDH, EIF5, CTPS1, CKB, GZMB, CASP7, CASP3, GZMM, TOM1L2, SEPT5, YWHAZ, YWHAG, PRKAA2, STRN4, NECAB2, CIDEB, EWSR1, NAP1L5, TMBIM4, USP15, HERC1, MCC, BAK
Protein Size: 331
Molecular Weight 37 kDa
Gene ID 1676
NCBI Accession NM_004401, NP_004392
UniProt O00273
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling ProductDetails: References for anti-DFFA antibody (ABIN2779329) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 331 AA

Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details ProductDetails: References for anti-DFFA antibody (ABIN2779329) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-DFFA antibody (ABIN2779329)

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling Images back to top
Background publications

Yan, Fu, Li, Blechl, Tranquilli, Bonafede, Sanchez, Valarik, Yasuda, Dubcovsky: "The wheat and barley vernalization gene VRN3 is an orthologue of FT." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 51, pp. 19581-6, 2006

Yan, Wang, Peng, Hu, Wang, Zhang, Chen, Bedford, Dewhirst, Li: "A unique role of the DNA fragmentation factor in maintaining genomic stability." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1504-9, 2006

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling ProductDetails: References for anti-DFFA antibody (ABIN2779329) back to top
Supplier Images
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (N-Term) (ABIN2779329) WB Suggested Anti-DFFA Antibody Titration: 5.0ug/ml ELISA Titer: 1:312500 Positive Co...