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anti-Mouse (Murine) Endonuclease G antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Endonuclease G Antibody (supplied by: Log in to see )

Antigen
Endonuclease G (ENDOG) Antibodies
  • CG8862
  • Dmel\\CG8862
  • ENDOG
  • endog
  • Tb08.10J17.90
  • Tb08.10J17.140
  • TBC1D13
  • wu:fb79c08
  • zgc:110020
Reactivity
Human, Mouse (Murine), Rat (Rattus)
97
58
52
3
1
1
1
Host
Rabbit
68
33
Clonality
Polyclonal
Conjugate
This Endonuclease G antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
79
33
15
13
11
9
5
3
2
2
1
1
Supplier
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Catalog No. ABIN499796
€ 382.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008985354 ABIN121687 IHC (p) WB Rabbit AA 55-70 Log in to see Polyclonal
0.0008985354 ABIN680893 IF (p) IHC (p) WB Rabbit IgG AA 110-150 Log in to see Polyclonal
0.0008985354 ABIN2473434 IHC (p) WB Rabbit IgG AA 55-70 Log in to see Polyclonal 2
0.0008985354 ABIN680895 IHC (p) WB Biotin Rabbit IgG AA 110-150 Log in to see Polyclonal
0.0008985354 ABIN680902 IHC (p) WB HRP Rabbit IgG AA 110-150 Log in to see Polyclonal
0.0008985354 ABIN320460 IHC (p) WB Rabbit IgG AA 55-70 Log in to see Polyclonal
0.0008985354 ABIN4308032 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0008985354 ABIN4308033 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-Endonuclease G antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Endonuclease G Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 25 Antibodies 19 Antibodies 33 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 5 Antibodies 6 Antibodies
Flow Cytometry (FACS) 1 Antibodies 3 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 9 Antibodies 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 9 Antibodies
Western Blotting (WB) 44 Antibodies 38 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies

General

Antigen Endonuclease G (ENDOG) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(97), (58), (52), (3), (1), (1), (1)
Host Rabbit
(68), (33)
Clonality
Polyclonal
Conjugate This Endonuclease G antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(79), (33), (15), (13), (11), (9), (5), (3), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-Endonuclease G Antibody

Target Details Endonuclease G Application Details Handling References for anti-Endonuclease G Antibody (ABIN499796) Images
Specificity EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.
Purification Affinity chromatography purified via peptide column
Immunogen Human EndoG Peptide
Isotype IgG

Target Details Endonuclease G

Product Details anti-Endonuclease G Antibody Application Details Handling References for anti-Endonuclease G Antibody (ABIN499796) Images back to top
Antigen
Alternative Name Endonuclease G (ENDOG Antibody Abstract)
Background The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).Synonyms: ENDOG
Gene ID 2021
NCBI Accession NP_004426
UniProt Q14249
Research Area DNA/RNA
Pathways Apoptosis

Application Details

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Handling References for anti-Endonuclease G Antibody (ABIN499796) Images back to top
Application Notes ELISA. Western 1 to 2 μg/mL. HepG2 and 3T3 cell lysates can be used as positive control and a 35 kDa band can be detected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Application Details References for anti-Endonuclease G Antibody (ABIN499796) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Endonuclease G Antibody (ABIN499796)

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Application Details Handling Images back to top
Background publications

Li, Luo, Wang: "Endonuclease G is an apoptotic DNase when released from mitochondria." in: Nature, Vol. 412, Issue 6842, pp. 95-9, 2001

Parrish, Li, Klotz, Ledwich, Wang, Xue: "Mitochondrial endonuclease G is important for apoptosis in C. elegans." in: Nature, Vol. 412, Issue 6842, pp. 90-4, 2001

Hengartner: "Apoptosis. DNA destroyers." in: Nature, Vol. 412, Issue 6842, pp. 27, 29, 2001

Widlak, Li, Wang, Garrard: "Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48404-9, 2001

Images

Product Details anti-Endonuclease G Antibody Target Details Endonuclease G Application Details Handling References for anti-Endonuclease G Antibody (ABIN499796) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Endonuclease G antibody (ENDOG) (ABIN499796) Immunohistochemistry of EndoG in human pancreas with EndoG antibody at 15 μg/ml.
Western Blotting (WB) image for anti-Endonuclease G antibody (ENDOG) (ABIN499796) Western blot analysis of EndoG in mouse (M) 3T3 and human (H) HepG2 cell lysates with...