anti-Mouse (Murine) PARP1 antibody for Immunohistochemistry (Frozen Sections)

Recommended PARP1 Antibody (supplied by: Log in to see )

Antigen
Poly (ADP-Ribose) Polymerase 1 (PARP1) Antibodies
  • BEST:LD21673
  • CG17685
  • CG17696
  • CG17718
  • CG40411
  • D.PARP
  • Dm.pARTa
  • Dmel\\CG40411
  • LD21673.3prime
  • PARP
  • PARP-1
  • PARP1
  • dPARP
  • parp
  • ADPRT
  • ADPRT 1
  • ADPRT1
  • ARTD1
  • PPOL
  • pADPRT-1
  • Adprt
  • Parp-1
  • adprt1
  • padprt-1
  • ppol
  • 5830444G22Rik
  • AI893648
  • Adprp
  • Adprt1
  • C80510
  • parp-1
  • sPARP-1
  • Poly-(ADP-ribose) polymerase
  • poly (ADP-ribose) polymerase 1
  • poly (ADP-ribose) polymerase family, member 1
  • poly ADP-ribose polymerase
  • Parp
  • PARP1
  • Parp1
  • parp1
Reactivity
Fruit Fly (Drosophila melanogaster), Hamster, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
385
83
79
18
17
13
9
9
6
5
4
3
2
2
1
1
1
1
1
Host
Mouse
378
45
1
Clonality
Monoclonal
Conjugate
This PARP1 antibody is un-conjugated
25
24
24
24
24
23
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
237
231
164
50
43
40
31
22
13
12
5
4
4
3
2
1
1
1
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Catalog No. ABIN118984
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1490558 ICC IHC (fro) IHC (p) IP ELISA WB Rabbit IgG AA 1-1014 Log in to see Polyclonal
1 ABIN462120 IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 Log in to see A6-4-12
1 ABIN2476185 IHC (fro) IF IHC (p) IP ELISA WB Mouse IgG1 Log in to see A6-4-12 4
1 ABIN600076 IHC (fro) IHC (p) IP ELISA WB Mouse IgG1 Log in to see

General

Antigen Poly (ADP-Ribose) Polymerase 1 (PARP1) Antibodies
Reactivity Fruit Fly (Drosophila melanogaster), Hamster, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(385), (83), (79), (18), (17), (13), (9), (9), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(378), (45), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PARP1 antibody is un-conjugated
(25), (24), (24), (24), (24), (23)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(237), (231), (164), (50), (43), (40), (31), (22), (13), (12), (5), (4), (4), (3), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-PARP1 Antibody

Target Details PARP1 Application Details Handling ProductDetails: References for anti-PARP1 antibody (ABIN118984) Images
Specificity This antibody recognises poly (ADP-ribose) polymerase (PARP).
Cross-Reactivity (Details) Species reactivity (expected):Xenopus, Drosophila, Hamster, Rat and Mouse.
Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G.
Immunogen Human PARP. Spleen cells from immunised BALB/c mice were fused with cells of mouse NSO myeloma cell line.
Clone A6-4-12
Isotype IgG1

Target Details PARP1

Product Details anti-PARP1 Antibody Application Details Handling ProductDetails: References for anti-PARP1 antibody (ABIN118984) Images back to top
Antigen
Alternative Name PARP1 (PARP1 Antibody Abstract)
Background PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular ''nick sensor'' and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1
Gene ID 142
NCBI Accession NP_001609
UniProt P09874
Pathways Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location

Application Details

Product Details anti-PARP1 Antibody Target Details PARP1 Handling ProductDetails: References for anti-PARP1 antibody (ABIN118984) Images back to top
Application Notes Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. ELISA. Immunoprecipitation. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PARP1 Antibody Target Details PARP1 Application Details ProductDetails: References for anti-PARP1 antibody (ABIN118984) Images back to top
Concentration 1.0 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

ProductDetails: References for anti-PARP1 antibody (ABIN118984)

Product Details anti-PARP1 Antibody Target Details PARP1 Application Details Handling Images back to top
Background publications

Bie, Yuan, Zhao, Zhang: "Method for estimating the axial intensity derivative in the TIE with higher order intensity derivatives and noise suppression." in: Optics express, Vol. 20, Issue 7, pp. 8186-91, 2012

Harris, Jakob, Taucher-Scholz, Dianov, Becherel, Lavin: "Aprataxin, poly-ADP ribose polymerase 1 (PARP-1) and apurinic endonuclease 1 (APE1) function together to protect the genome against oxidative damage." in: Human molecular genetics, Vol. 18, Issue 21, pp. 4102-17, 2009

Freire, dAdda Di Fagagna, Wu, Pedrazzi, Stagljar, Hickson, Jackson: "Cleavage of the Bloom's syndrome gene product during apoptosis by caspase-3 results in an impaired interaction with topoisomerase IIIalpha." in: Nucleic acids research, Vol. 29, Issue 15, pp. 3172-80, 2001

Krohn, Preis, Prehn: "Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 20, pp. 8186-97, 1998