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|Antigen||Poly (ADP-Ribose) Polymerase 1 (PARP1) Antibodies|
|Reactivity||Fruit Fly (Drosophila melanogaster), Hamster, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis Alternatives|
|Conjugate||This PARP1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|4 references available|
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Product Details anti-PARP1 AntibodyTarget Details PARP1 Application Details Handling References for anti-PARP1 antibody (ABIN118984) Images
|Specificity||This antibody recognises poly (ADP-ribose) polymerase (PARP).|
Species reactivity (expected):Xenopus, Drosophila, Hamster, Rat and Mouse.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography on Protein G.|
|Immunogen||Human PARP. Spleen cells from immunised BALB/c mice were fused with cells of mouse NSO myeloma cell line.|
Target Details PARP1Product Details anti-PARP1 Antibody Application Details Handling References for anti-PARP1 antibody (ABIN118984) Images back to top
|Alternative Name||PARP1 (PARP1 Antibody Abstract)|
|Background||PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular ''nick sensor'' and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location|
Application DetailsProduct Details anti-PARP1 Antibody Target Details PARP1 Handling References for anti-PARP1 antibody (ABIN118984) Images back to top
Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. ELISA. Immunoprecipitation. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PARP1 Antibody Target Details PARP1 Application Details References for anti-PARP1 antibody (ABIN118984) Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.|
References for anti-PARP1 antibody (ABIN118984)Product Details anti-PARP1 Antibody Target Details PARP1 Application Details Handling Images back to top
Bie, Yuan, Zhao, Zhang: "Method for estimating the axial intensity derivative in the TIE with higher order intensity derivatives and noise suppression." in: Optics express, Vol. 20, Issue 7, pp. 8186-91, 2012
Harris, Jakob, Taucher-Scholz, Dianov, Becherel, Lavin: "Aprataxin, poly-ADP ribose polymerase 1 (PARP-1) and apurinic endonuclease 1 (APE1) function together to protect the genome against oxidative damage." in: Human molecular genetics, Vol. 18, Issue 21, pp. 4102-17, 2009
Freire, dAdda Di Fagagna, Wu, Pedrazzi, Stagljar, Hickson, Jackson: "Cleavage of the Bloom's syndrome gene product during apoptosis by caspase-3 results in an impaired interaction with topoisomerase IIIalpha." in: Nucleic acids research, Vol. 29, Issue 15, pp. 3172-80, 2001
Krohn, Preis, Prehn: "Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 20, pp. 8186-97, 1998