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anti-Mouse (Murine) Perforin 1 antibody for Flow Cytometry

Recommended Perforin 1 Antibody (supplied by: Log in to see )

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • Cyta
  • FLH2
  • HPLH2
  • LOC443187
  • P1
  • perforin
  • Pfn
  • PFN1
  • Pfp
  • PFP
  • Prf-1
  • prf1
  • PRF1
  • RATCYTA
Reactivity
Mouse (Murine)
162
47
21
18
1
1
Host
Rat
108
72
27
Clonality
Monoclonal
Conjugate
This Perforin 1 antibody is conjugated to APC
18
13
10
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
92
78
62
59
45
44
44
42
31
8
6
6
3
2
2
1
1
1
1
1
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Catalog No. ABIN282883
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010463598 ABIN279223 FACS ICS PE Rat IgG2a kappa Log in to see OMAK-D 1
0.0010463598 ABIN278221 FACS ICS FITC Rat IgG2a kappa Log in to see OMAK-D 1
0.0010463598 ABIN187239 EIA FACS IHC (fro) IF WB Rat IgG2a AA 189-360 Log in to see KM585 (P1-8) 4
0.0010463598 ABIN4344818 FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4

Top referenced anti-Perforin 1 antibody for Flow Cytometry

Similar anti-Perforin 1 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 6 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 36 Antibodies

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Reactivity Mouse (Murine)
(162), (47), (21), (18), (1), (1)
Host Rat
(108), (72), (27)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Perforin 1 antibody is conjugated to APC
(18), (13), (10), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(92), (78), (62), (59), (45), (44), (44), (42), (31), (8), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 Antibody (ABIN282883) Images
Specificity The antibody reacts with mouse perforin (pore-forming protein, pfp, Prf). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

By immunobloting, recognizes a ~70 kDa band in lysates of CTLL-2 mouse cytotoxic cell line and in lysates of IL-2 stimulated but not unstimulated mouse splenocytes. By multi-color intracellular flow cytometric analysis, eBioOMAK-D staining is increased upon stimulation (IL-2 or anti-CD3/28). Intracellular flow staining results showing upregulation of protein expression have been confirmed by immunoblotting. Furthermore, stimulated Perforin Knock-out (developed by Walsh) splenocytes do not stain with eBioOMAK-D nor is any protein detectable by western blotting with eBioOMAK-D as well as other anti-mouse perforin antibodies. Please note that the Kagi perforin knock-out mice may synthesize a truncated form of the protein which may be recognized by eBioOMAK-D.

In IL-2 stimulated mouse splenocytes, NK cells (as determined by CD49b staining) contain perforin while CD8 cells contain little to none and can vary with culture conditions. This has been confirmed by staining and western blotting the two populations using both OMAK-D and P1-8 antibodies. In contrast stimulation of splenocytes with anti-CD3/CD28 antibodies does result in an increase of perforin on both NK cells and CD8 cells.

eBioOMAK-D is also crossreactive to human perforin and co-stains CD56 positive cells in PBMC.

Expression of perforin and Granzyme B do not always correlate (as discussed above in the CD8 population of IL-2 stimulated splenocytes). Granzyme B typically is expressed earlier and at higher levels. Expression of Granzyme B is dramatically increased (more than 10,00 fold based on mRNA estimates and significantly at the protein level based on western blotting and flow analysis) compared to a minimal increase (10-100 fold) in perforin mRNA and protein with IL-2 stimulation.

For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse perforin, it is highly recommended to use the Foxp3 buffer system . Other buffers may yield varying results.
Characteristics The eBioOMAK-D antibody reacts with mouse perforin (pore-forming protein, pfp, Prf). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections. By immunobloting, eBioOMAK-D recognizes a ~70 kDa band in lysates of CTLL-2 mouse cytotoxic cell line and in lysates of IL-2 stimulated but not unstimulated mouse splenocytes. By multi-color intracellular flow cytometric analysis, eBioOMAK-D staining is increased upon stimulation (IL-2 or anti-CD3/28). Intracellular flow staining results showing upregulation of protein expression have been confirmed by immunoblotting. Furthermore, stimulated Perforin Knock-out (developed by Walsh) splenocytes do not stain with eBioOMAK-D nor is any protein detectable by western blotting with eBioOMAK-D as well as other anti-mouse perforin antibodies. Please note that the Kagi perforin knock-out mice may synthesize a truncated form of the protein which may be recognized by eBioOMAK-D. In IL-2 stimulated mouse splenocytes, NK cells (as determined by CD49b staining) contain perforin while CD8 cells contain little to none and can vary with culture conditions. This has been confirmed by staining and western blotting the two populations using both OMAK-D and P1-8 antibodies. In contrast stimulation of splenocytes with anti-CD3/CD28 antibodies does result in an increase of perforin on both NK cells and CD8 cells. eBioOMAK-D is also crossreactive to human perforin and co-stains CD56 positive cells in PBMC. Expression of perforin and Granzyme B do not always correlate (as discussed above in the CD8 population of IL-2 stimulated splenocytes). Granzyme B typically is expressed earlier and at higher levels. Expression of Granzyme B is dramatically increased (more than 10,00 fold based on mRNA estimates and significantly at the protein level based on western blotting and flow analysis) compared to a minimal increase (10-100 fold) in perforin mRNA and protein with IL-2 stimulation. For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse perforin, it is highly recommended to use the Foxp3 buffer system (cat. 00-5523). Other buffers may yield varying results. For more information, please contact technical support at tech@ebioscience.com.
Clone OMAK-D
Isotype IgG2a kappa

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling References for anti-Perforin 1 Antibody (ABIN282883) Images back to top
Antigen
Alternative Name Perforin (PRF1 Antibody Abstract)
Background Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections. By immunobloting, OMAK-D recognizes a approx. 70kDa band in lysates of CTLL-2 mouse cytotoxic cell line and in lysates of IL-2 stimulated but not unstimulated mouse splenocytes. By multi-color intracellular flow cytometric analysis, OMAK-D staining is increased upon stimulation (IL-2 or anti-CD3/28). Intracellular flow staining results showing upregulation of protein expression have been confirmed by immunoblotting. Furthermore, stimulated Perforin Knock-out (developed by Walsh) splenocytes do not stain with OMAK-D nor is any protein detectable by western blotting with OMAK-D as well as other anti-mouse perforin antibodies. Please note that the Kagi perforin knock-out mice may synthesize a truncated form of the protein which may be recognized by OMAK-D. In IL-2 stimulated mouse splenocytes, NK cells (as determined by CD49b staining) contain perforin while CD8 cells contain little to none and can vary with culture conditions. This has been confirmed by staining and western blotting the two populations using both OMAK-D and P1-8 antibodies. In contrast stimulation of splenocytes with anti-CD3/CD28 antibodies does result in an increase of perforin on both NK cells and CD8 cells. OMAK-D is also crossreactive to human perforin and co-stains CD56 positive cells in PBMC.Expression of perforin and Granzyme B do not always correlate (as discussed above in the CD8 population of IL-2 stimulated splenocytes). Granzyme B typically is expressed earlier and at higher levels. Expression of Granzyme B is dramatically increased (more than 10,00 fold based on mRNA estimates and significantly at the protein level based on western blotting and flow analysis) compared to a minimal increase (10-100 fold) in perforin mRNA and protein with IL-2 stimulation.
Research Area Immunology, Innate Immunity, Adaptive Immunity, Inflammation
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling References for anti-Perforin 1 Antibody (ABIN282883) Images back to top
Application Notes Applications reported: This eBioOMAK-D antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This eBioOMAK-D antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Please refer to Best Protocols: Protocol B: One step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Comment

Excitation/Emission wavelength: 650 nm/660 nm

Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details References for anti-Perforin 1 Antibody (ABIN282883) Images back to top
Concentration 0.2 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light sensitive material.
Storage 4 °C

References for anti-Perforin 1 Antibody (ABIN282883)

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling Images back to top
Product cited in:

Fehniger, Cai, Cao, Bredemeyer, Presti, French, Ley: "Acquisition of murine NK cell cytotoxicity requires the translation of a pre-existing pool of granzyme B and perforin mRNAs." in: Immunity, Vol. 26, Issue 6, pp. 798-811, 2007

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 Antibody (ABIN282883) back to top
Supplier Images
 image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein))  (APC) (ABIN282883) Staining of fixed and permeabilized C57BL/6 splenocytes cultured with IL-2 for 4 days...