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anti-Mouse (Murine) Perforin 1 antibody for Immunohistochemistry (Frozen Sections)

Recommended Perforin 1 Antibody (supplied by: Log in to see )

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • Cyta
  • FLH2
  • HPLH2
  • LOC443187
  • P1
  • perforin
  • Pfn
  • PFN1
  • Pfp
  • PFP
  • Prf-1
  • prf1
  • PRF1
  • RATCYTA
Epitope
AA 189-360
9
9
6
6
5
4
3
3
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
143
31
17
8
1
1
Host
Rat
92
70
13
Clonality
Monoclonal
Conjugate
This Perforin 1 antibody is un-conjugated
18
11
10
4
4
3
3
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
73
63
34
31
30
16
16
16
14
9
6
6
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN187239
$ 553.67
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN1860312 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 40-355 Log in to see Polyclonal
0.00088051456 ABIN199825 ICC IHC (fro) IHC (p) IP WB Rat IgG2a AA 98-534 Log in to see CB5-4
0.00088051456 ABIN187561 IHC (fro) IF IHC (p) WB Rat IgG2a Log in to see CB5-4

Top referenced anti-Perforin 1 antibody for Immunohistochemistry (Frozen Sections)

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Flow Cytometry (FACS) 4 Antibodies
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Immunohistochemistry (IHC) 7 Antibodies
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ELISpot 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Epitope AA 189-360
(9), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(143), (31), (17), (8), (1), (1)
Host Rat
(92), (70), (13)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Perforin 1 antibody is un-conjugated
(18), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(73), (63), (34), (31), (30), (16), (16), (16), (14), (9), (6), (6), (3), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 Antibody (ABIN187239) Images
Specificity Reacts with the common epitope of perforin existing in NK cells and CTL cells. Recognizes mouse perforin, cross-reacts with human perforin. Species Reactivity: Mouse and human. Others not tested.
Purification Ascites
Immunogen Recombinant mouse perforin fragment encompassing 172 amino acid residues (His189-Cys360) which contains the membrane binding domain and part of the EGF-type cysteine-rich domain.Mouse myeloma (P3U/I) x immunized SD rat spleen cells.
Clone KM585 (P1-8)
Isotype IgG2a

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling References for anti-Perforin 1 Antibody (ABIN187239) Images back to top
Antigen
Alternative Name Perforin 1 / PRF1 (PRF1 Antibody Abstract)
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling References for anti-Perforin 1 Antibody (ABIN187239) Images back to top
Application Notes ELISA. Western blot. Immunohistochemistry: Frozen tissue sections and cytospins. Use at a 1:50-1:200 dilution. It is recommended that treatment with H2O2 in water be substituted for H2O2 in methanol. Flow cytometry: Successful application of clone KM585 (P1-8) to flow cytometry has been reported by some users. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details References for anti-Perforin 1 Antibody (ABIN187239) Images back to top
Format Liquid
Buffer 100 µL of monoclonal antibody in ascites fluid containing 0.1% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-Perforin 1 Antibody (ABIN187239)

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling Images back to top
Background publications

Urrea Moreno, Gil, Rodriguez-Sainz, Cela, LaFay, Oloizia, Herr, Sumegi, Jordan, Risma: "Functional assessment of perforin C2 domain mutations illustrates the critical role for calcium-dependent lipid binding in perforin cytotoxic function." in: Blood, Vol. 113, Issue 2, pp. 338-46, 2009

Araki, Fann, Wersto, Weng: "Histone acetylation facilitates rapid and robust memory CD8 T cell response through differential expression of effector molecules (eomesodermin and its targets: perforin and granzyme B)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 12, pp. 8102-8, 2008

Motegi, Kinoshita, Inatsu, Habu, Saitoh, Seki: "IL-15-induced CD8+CD122+ T cells increase antibacterial and anti-tumor immune responses: implications for immune function in aged mice." in: Journal of leukocyte biology, Vol. 84, Issue 4, pp. 1047-56, 2008

Saha, Bhattacharya-Chatterjee, Foon, Celis, Chatterjee: "Stimulatory effects of CpG oligodeoxynucleotide on dendritic cell-based immunotherapy of colon cancer in CEA/HLA-A2 transgenic mice." in: International journal of cancer. Journal international du cancer, Vol. 124, Issue 4, pp. 877-88, 2008