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anti-Mouse (Murine) Perforin 1 antibody for Immunohistochemistry (Frozen Sections)

Recommended Perforin 1 Antibody (supplied by: Log in to see )

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • PRF1
  • LOC443187
  • perforin
  • prf1
  • FLH2
  • HPLH2
  • P1
  • PFN1
  • PFP
  • Pfn
  • Pfp
  • Prf-1
  • Cyta
  • RATCYTA
  • perforin 1 (pore forming protein)
  • perforin
  • perforin 1-like
  • PRF1
  • LOC443187
  • prf1
  • Prf1
  • LOC100342396
Epitope
AA 402-534, AA 98-534
22
15
9
6
6
5
3
3
3
3
3
2
1
1
1
1
1
Reactivity
Mouse (Murine)
161
49
23
20
2
1
1
Host
Rat
101
75
22
Clonality
Monoclonal
Conjugate
This Perforin 1 antibody is un-conjugated
17
11
10
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
100
82
44
40
38
36
35
35
32
6
6
6
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN4344818
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4344832 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4 1
1 ABIN187239 EIA FACS IHC (fro) IF WB Rat IgG2a AA 189-360 Log in to see KM585 (P1-8) 4
1 ABIN4344822 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
1 ABIN4344826 ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
1 ABIN4344842 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
1 ABIN4344838 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
1 ABIN4344836 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
1 ABIN4344840 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
1 ABIN4344820 FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
1 ABIN4344830 FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
1 ABIN4344844 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Rat IgG2a AA 402-534, AA 98-534 Log in to see CB5-4
1 ABIN199825 ICC IHC (fro) IHC (p) IP WB Rat IgG2a AA 98-534 Log in to see CB5-4
1 ABIN187561 IHC (fro) IF IHC (p) WB Rat IgG2a Log in to see CB5-4

Top referenced anti-Perforin 1 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Perforin 1 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 6 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Western Blotting (WB) 43 Antibodies

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Epitope AA 402-534, AA 98-534
(22), (15), (9), (6), (6), (5), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(161), (49), (23), (20), (2), (1), (1)
Host Rat
(101), (75), (22)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Perforin 1 antibody is un-conjugated
(17), (11), (10), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(100), (82), (44), (40), (38), (36), (35), (35), (32), (6), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 Antibody (ABIN4344818) Images
Specificity Recognizes mouse perforin (region aa 402-534).
Purification Protein G purified
Immunogen Recombinant mouse perforin (aa 98-534).
Clone CB5-4
Isotype IgG2a

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling References for anti-Perforin 1 Antibody (ABIN4344818) Images back to top
Antigen
Alternative Name Perforin (PRF1 Antibody Abstract)
Background Gene Symbol: PRF1
Gene ID 5551
Research Area Adaptive Immunity, Immunology, Innate Immunity, Inflammation
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling References for anti-Perforin 1 Antibody (ABIN4344818) Images back to top
Application Notes Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500This antbody is useful in Immunocytochemistry, Immunohistochemistry (frozen sections, paraffin sections), Immunoprecipitation and Western Blot.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details References for anti-Perforin 1 Antibody (ABIN4344818) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Perforin 1 Antibody (ABIN4344818)

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling Images back to top
Product cited in:

Rah, Kwak, Chung, Kim: "ADP-ribose/TRPM2-mediated Ca2+ signaling is essential for cytolytic degranulation and antitumor activity of natural killer cells." in: Scientific reports, Vol. 5, pp. 9482, 2015 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Dalton, Howell, Pearson, Scott, Carding: "Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3660-7, 2004

Crider-Pirkle, Billingsley, Faust, Hardy, Lee, Weitlauf: "Cubilin, a binding partner for galectin-3 in the murine utero-placental complex." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 15904-12, 2002

Müller, Tschopp: "Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 6, pp. 2470-8, 1994

Dupuis, Schaerer, Krause, Tschopp: "The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes." in: The Journal of experimental medicine, Vol. 177, Issue 1, pp. 1-7, 1993

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 Antibody (ABIN4344818) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (AA 402-534) (ABIN4344818) Flow Cytometry: Perforin Antibody (CB5.4) [NBP1-97512] - Analysis of 106 Jurkat cells...
Western Blotting (WB) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (AA 402-534) (ABIN4344818) Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the m...