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SMAD4 Antibodies

(SMAD Family Member 4 (SMAD4))

Choose one of 105 SMAD4 antibodies from our product listing based on 10 publications and 89 validation images.

Explore reliable SMAD4 antibodies from a broad selection of renowned manufacturers. Our portfolio enables accurate SMAD4 detection across multiple species, including Human, Mouse, Rat, Monkey, Cow, Rabbit, Zebrafish (Danio rerio), Dog, Goat, Guinea Pig, Horse, Sheep, Drosophila melanogaster, Pig, Xenopus laevis, and supports diverse research applications such as WB, ELISA, IHC, IP, IF.

Quickly find the right antibody using our search, filter, and comparison tools. Each product page provides detailed validation data, literature references, and customer feedback. Unlike comparison-only resources, you can conveniently purchase antibodies directly through our site. Choose from polyclonal, monoclonal, or recombinant SMAD4 antibodies derived from various hosts such as Rabbit, Mouse, Goat. Browse our products below and order directly to support your research with confidence.

Alternative names for SMAD4 are SMAD4, smad4, Smad4, smad4.1.L.

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105 results

SMAD4 antibody (N-Term)

SMAD4 Reactivity: Human WB, IP, CUT&RUN Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-SMAD Family Member 4 (SMAD4) (N-Term) antibody (ABIN6972727)

Cleavage Under Targets and Release Using Nuclease validation image for anti-SMAD Family Member 4 (SMAD4) (N-Term) antibody (ABIN6972727)

SMAD4 antibody (pAb) tested by Western blot.

SMAD4 antibody (pAb) tested by Immunoprecipitation.

4 images

1 reference

Independent Validation

1 validation

Catalog No. ABIN6972727

Datasheet as PDF

Independently Validated

SMAD4 antibody

KO Validated SMAD4 Reactivity: Human WB, IHC, IP Host: Rabbit Monoclonal unconjugated

Western blot analysis of extracts from wild type (WT) and Smad4 knockout (KO) 293T cells, using Smad4 antibody (ABIN7270340) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human liver cancer using [KO Validated] Smad4 Rabbit mAb (ABIN7270340) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using Smad4 antibody (ABIN7270340) at 1:1000 dilution.

Western blot analysis of extracts of various cell lines, using Smad4 antibody (ABIN7270340) at 1:1000 dilution.

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Smad4 antibody (ABIN7270340).

Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using [KO Validated] Smad4 Rabbit mAb antibody (ABIN7270340) and rabbit IgG.

6 images

Catalog No. ABIN7270340

Datasheet as PDF

SMAD4 antibody (AA 1-552)

SMAD4 Reactivity: Human WB, ELISA, IF, PLA Host: Mouse Monoclonal 3E2 unconjugated

SMAD4 monoclonal antibody (M02), clone 3E2 Western Blot analysis of SMAD4 expression in Hela S3 NE .

Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell.

SMAD4 monoclonal antibody (M02), clone 3E2.

SMAD4 monoclonal antibody (M02), clone 3E2.

Detection limit for recombinant GST tagged SMAD4 is approximately 3ng/ml as a capture antibody.

Proximity Ligation Analysis of protein-protein interactions between CDKN1A and SMAD4.

Western Blot detection against Immunogen (86.

Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell.

8 images

Catalog No. ABIN561723

Datasheet as PDF

SMAD4 antibody (AA 255-284)

SMAD4 Reactivity: Human, Mouse WB, IF Host: Rabbit Polyclonal RB14714 unconjugated

Western blot analysis of SD4 Antibody (arrow) using rabbit polyclonal SD4 Antibody (ABIN392165 and ABIN2841882).

All lanes : Anti-SD4 Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Neuro-2a whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane.

Anti-SD4 Antibody at 1:2000 dilution + Neuro-2a whole cell lysate Lysates/proteins at 20 μg per lane.

Confocal immunofluorescent analysis of SD4 Antibody (ABIN392165 and ABIN2841882) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

4 images

1 reference

Catalog No. ABIN392165

Datasheet as PDF

SMAD4 antibody

SMAD4 Reactivity: Human, Mouse WB, ELISA, IHC, ICC, FACS Host: Mouse Monoclonal 4G1C6 unconjugated

Western blot analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.

Flow cytometric analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).

Immunofluorescence analysis of NIH/3T3 cells using SMAD4 mouse mAb (green).

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SMAD4 mouse mAb with DAB staining.

4 images

2 references

Catalog No. ABIN969403

Datasheet as PDF

SMAD4 antibody (AA 400-428)

SMAD4 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Polyclonal unconjugated

Western blot analysis of SMAD4 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).

IHC analysis of FFPE human skeletal muscle stained with SMAD4 antibody

Fluorescent confocal image of HeLa cell stained with SMAD4 antibody at 1:25.

Western blot analysis of SMAD4 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).

Fluorescent confocal image of HeLa cell stained with SMAD4 antibody at 1:25.

SMAD4 antibody flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control (top histogram).

6 images

Catalog No. ABIN3032608

Datasheet as PDF

SMAD4 antibody (AA 27-195)

SMAD4 Reactivity: Human WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated

Western blot All lanes: SMAD4 antibody at 3 μg/mL + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using ABIN7160238 at dilution of 1:100

Immunoprecipitating SMAD4 in Jurkat whole cell lysate Lane 1: Rabbit control IgG (1 μg) instead of ABIN7160238 in Jurkat whole cell lysate.

Immunohistochemistry of paraffin-embedded human colon cancer using ABIN7160238 at dilution of 1:100

4 images

Catalog No. ABIN7160238

Datasheet as PDF

SMAD4 antibody (C-Term)

SMAD4 Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB20720 unconjugated

Western blot analysis of SD4 (arrow) using rabbit polyclonal SD4 Antibody (C-term) (ABIN652388 and ABIN2841883).

Forlin-fixed and paraffin-embedded hun skeletal muscle reacted with SD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Fluorescent confocal ige of Hela cell stained with SD4 Antibody (C-term) (ABIN652388 and ABIN2841883).

SD4 Antibody (C-term) (ABIN652388 and ABIN2841883) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).

4 images

Catalog No. ABIN652388

Datasheet as PDF

SMAD4 antibody (pThr277)

SMAD4 Reactivity: Human ELISA, IHC, IF, DB Host: Rabbit Polyclonal unconjugated

IHC analysis of FFPE breast carcinoma stained with phospho-SMAD4 antibody.

Confocal immunofluorescent analysis of phospho-SMAD4 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Dot blot analysis of phospho-SMAD4 antibody.

IHC analysis of FFPE breast carcinoma stained with phospho-SMAD4 antibody.

Confocal immunofluorescent analysis of phospho-SMAD4 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

5 images

Catalog No. ABIN3032606

Datasheet as PDF

SMAD4 antibody (AA 9-231)

SMAD4 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Detection of Smad4 in Human MCF7 cell lysate using Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)

Detection of Recombinant Smad4, Mouse using Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)

Detection of Smad4 in Mouse Testis Tissue using Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)

3 images

Catalog No. ABIN7441925

Datasheet as PDF

SMAD4 antibody

SMAD4 Reactivity: Human, Mouse, Rat ELISA, IHC Host: Rabbit Polyclonal unconjugated

Western Blot analysis of Human liver cancer tissue and Hela cells using SMAD4 Polyclonal Antibody at dilution of 1:150

Immunohistochemistry of paraffin-embedded Human cervical cancer using SMAD4 Polyclonal Antibody at dilution of 1/40

Immunohistochemistry of paraffin-embedded Human thyroid cancer using SMAD4 Polyclonal Antibody at dilution of 1/40

3 images

Catalog No. ABIN7243955

Datasheet as PDF

SMAD4 antibody

SMAD4 Reactivity: Human, Mouse, Rat WB, IHC (p), IF (cc) Host: Rabbit Monoclonal 3A1 unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer at 37°C for 20min, Antibody incubation with Smad4 (3A1) Monoclonal Antibody, at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Lane 1: PC-12 lysates probed with Smad4 (3A1) Monoclonal Antibody at 1:1000 overnight at 4˚C.

Hela cells were stained with Smad4 (3A1) Monoclonal Antibody at [1:200] incubated overnight at 4C followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

3 images

Catalog No. ABIN5557399

Datasheet as PDF

SMAD4 antibody (AA 56-165)

SMAD4 Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 3D7 unconjugated

Western Blot detection against Immunogen (37.

Detection limit for recombinant GST tagged SMAD4 is approximately 3ng/ml as a capture antibody.

Proximity Ligation Analysis of protein-protein interactions between AKT1 and SMAD4.

3 images

Catalog No. ABIN517638

Datasheet as PDF

SMAD4 antibody (N-Term)

SMAD4 Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Goat, Guinea Pig, Horse, Sheep WB, IHC Host: Rabbit Polyclonal unconjugated

WB Suggested Anti-SMAD4 Antibody Titration: 0.

Host: Mouse Target Name: SMAD4 Sample Tissue: Mouse Kidney Antibody Dilution: 1ug/ml

Rabbit Anti-SMAD4 antibody Paraffin Embedded Tissue: Human Lung cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.

Rabbit Anti-SMAD4 antibody Formalin Fixed Paraffin Embedded Tissue: Human Adult Small intestine Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.

4 images

Catalog No. ABIN2780673

Datasheet as PDF

SMAD4 antibody (AA 160-450)

SMAD4 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts of various cell lines, using SMAD4 antibody.

Immunofluorescence analysis of U2OS cells using SMAD4 antibody.

2 images

Catalog No. ABIN6148088

Datasheet as PDF

SMAD4 antibody

SMAD4 Reactivity: Human, Mouse, Rat, Monkey ELISA, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

Immunofluorescence analysis of Rat lung tissue using Smad4 Polyclonal Antibody at dilution of 1:200.

Immunohistochemistry of paraffin-embedded Mouse testis tissue using Smad4 Polyclonal Antibody at dilution of 1:200.

2 images

Catalog No. ABIN7251286

Datasheet as PDF

SMAD4 antibody (pThr277)

SMAD4 Reactivity: Human, Mouse WB Host: Rabbit Polyclonal RB07971 unconjugated

Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-β(100 ng/mL, 30 min), using Phospho-SD4 Antibody (upper) or Beta-actin (lower).

1 image

1 reference

Catalog No. ABIN389658

Datasheet as PDF

SMAD4 antibody (N-Term)

SMAD4 Reactivity: Human, Mouse, Rat, Monkey, Cow, Zebrafish (Danio rerio), Pig WB, IHC, IP, IF, IC Host: Rabbit Polyclonal unconjugated

Western blot analysis of SMAD4 expression in HepG2 (A), HeLa (B), NIH3T3 (C), COS7 (D) whole cell lysates.

Immunofluorescent analysis of SMAD4 staining in HeLa cells.

Immunohistochemical analysis of SMAD4 staining in human breast cancer formalin fixed paraffin embedded tissue section.

3 images

Catalog No. ABIN7296234

Datasheet as PDF

SMAD4 antibody (AA 255-284)

SMAD4 Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated

Western blot analysis of SMAD4 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2).

Confocal immunofluorescent analysis of SMAD4 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Confocal immunofluorescent analysis of SMAD4 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

3 images

Catalog No. ABIN3032604

Datasheet as PDF

SMAD4 antibody

SMAD4 Reactivity: Human, Mouse, Rat WB, IHC (p), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Smad4 Polyclonal Antibody, Unconjugated (ABIN670491) at 1:200, followed by conjugation to the secondary antibody and DAB staining

1 image

Catalog No. ABIN670491

Datasheet as PDF

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