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anti-Human CD55 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • AI323748
  • Cd55b
  • CR
  • CROM
  • Daf
  • DAF
  • Daf-GPI
  • Daf-TM
  • Daf1
  • GPI-DAF
  • TC
  • TM-DAF
Reactivity
Human
287
35
9
7
6
4
3
1
Host
Mouse
220
86
8
7
6
3
2
Clonality
Monoclonal
Conjugate
This CD55 antibody is un-conjugated
49
45
25
10
4
3
3
3
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
225
137
63
61
41
36
34
25
10
5
4
3
3
2
1
1
1
1
Supplier
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Catalog No. ABIN118737
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN118738 FACS IHC (fro) Mouse IgG1 Log in to see 67 4
0.00088051456 ABIN2145187 IHC (fro) FACS WB Mouse IgG1 Log in to see 4A143-30 2
0.00088051456 ABIN319782 FACS IHC (fro) Mouse IgG1 Log in to see 67 4
0.00088051456 ABIN2145185 IHC (fro) FACS WB Mouse IgG1 Log in to see 4A143-30 2
0.00088051456 ABIN2144640 IHC (fro) FACS WB FITC Mouse IgG1 Log in to see 4A143-30 2
0.00088051456 ABIN2144643 IHC (fro) FACS WB FITC Mouse IgG1 Log in to see 4A143-30 2
0.00088051456 ABIN2144924 IHC (fro) FACS WB PE Mouse IgG1 Log in to see 4A143-30 2
0.00088051456 ABIN2144926 IHC (fro) FACS WB PE Mouse IgG1 Log in to see 4A143-30 2
0.00088051456 ABIN2480958 IHC (fro) FACS WB Mouse IgG1 Log in to see 67 3
0.00088051456 ABIN211859 FACS IHC (fro) Mouse IgG1 Log in to see
0.00088051456 ABIN198278 FACS IHC (fro) Mouse IgG1 Log in to see 67
0.00088051456 ABIN203999 FACS IHC (fro) Mouse IgG1 Log in to see
0.00088051456 ABIN1838119 IHC (fro) FACS Mouse IgG1 Log in to see 67
0.00088051456 ABIN371558 FACS IHC (fro) Mouse IgG1 Log in to see 143-30 2
0.00088051456 ABIN600850 IHC (fro) FACS IHC (p) Mouse IgG1 Log in to see
0.00088051456 ABIN600851 IHC (fro) FACS IHC (p) Mouse IgG1 Log in to see
0.00088051456 ABIN2689337 IHC (fro) FACS Mouse IgG2a, kappa Log in to see IA10 1
0.00088051456 ABIN333738 FACS IHC (fro) Biotin Mouse IgG1 Log in to see
0.00088051456 ABIN232523 FACS IHC (fro) Cy5,PE Mouse IgG1 Log in to see
0.00088051456 ABIN232520 FACS IHC (fro) FITC Mouse IgG1 Log in to see

Top referenced anti-CD55 antibody for Immunohistochemistry (Frozen Sections)

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Application / Reactivity Human
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Functional Studies (Func) 3 Antibodies
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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 61 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
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General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Reactivity Human
(287), (35), (9), (7), (6), (4), (3), (1)
Host Mouse
(220), (86), (8), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is un-conjugated
(49), (45), (25), (10), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(225), (137), (63), (61), (41), (36), (34), (25), (10), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling References for anti-CD55 Antibody (ABIN118737) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G.
Immunogen K562 cells
Clone 67
Isotype IgG1

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling References for anti-CD55 Antibody (ABIN118737) Images back to top
Antigen
Alternative Name CD55 / DAF (CD55 Antibody Abstract)
Background CD55 is the complement regulatory protein, decay accelerating factor (DAF). It is a 70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin. CD55 is expressed by a wide range of cell types.Synonyms: Complement decay-accelerating factor
Gene ID 9606
UniProt P08174
Pathways Complement System

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling References for anti-CD55 Antibody (ABIN118737) Images back to top
Application Notes Flow Cytometry: Use 10 μL of 1/10-1/25 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control Tissue: Human Tonsil. (Successful use in immunohistochemistry on paraffin embedded sections (1/50-1/100using heat treatment for antigen retrieval) was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details References for anti-CD55 Antibody (ABIN118737) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, with 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD55 Antibody (ABIN118737)

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling Images back to top
Product cited in:

Wenzel, Zabojszcza, Carl, Taubert, Lass, Harris, Ho, Schulz, Hummel, Hubner, Osterziel, Spuler: "Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6219-25, 2005

Background publications

Holmes, Simpson, Wainwright, Tate, Houlihan, Sawyer, Rogers, Spring, Anstee, Tanner: "Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 8, pp. 3099-105, 1990

Lublin, Atkinson: "Decay-accelerating factor: biochemistry, molecular biology, and function." in: Annual review of immunology, Vol. 7, pp. 35-58, 1989

Daniels: "Cromer-related antigens--blood group determinants on decay-accelerating factor." in: Vox sanguinis, Vol. 56, Issue 4, pp. 205-11, 1989

Images

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling References for anti-CD55 Antibody (ABIN118737) back to top
Supplier Images
 image for anti-CD55 antibody (Complement Decay-Accelerating Factor) (ABIN118737) Staining of human peripheral blood monocytes with Mouse anti Human CD55 (ABIN118737).