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|Antigen||Complement Decay-Accelerating Factor (CD55) Antibodies|
|Conjugate||This CD55 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
|4 references available|
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Product Details anti-CD55 AntibodyTarget Details CD55 Application Details Handling ProductDetails: References for anti-CD55 antibody (ABIN118737) Images
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Affinity Chromatography on Protein G.|
Target Details CD55Product Details anti-CD55 Antibody Application Details Handling ProductDetails: References for anti-CD55 antibody (ABIN118737) Images back to top
|Alternative Name||CD55 / DAF (CD55 Antibody Abstract)|
|Background||CD55 is the complement regulatory protein, decay accelerating factor (DAF). It is a 70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin. CD55 is expressed by a wide range of cell types.Synonyms: Complement decay-accelerating factor|
|Pathways||Complement System, Regulation of Leukocyte Mediated Immunity|
Application DetailsProduct Details anti-CD55 Antibody Target Details CD55 Handling ProductDetails: References for anti-CD55 antibody (ABIN118737) Images back to top
Flow Cytometry: Use 10 μL of 1/10-1/25 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control Tissue: Human Tonsil. (Successful use in immunohistochemistry on paraffin embedded sections (1/50-1/100using heat treatment for antigen retrieval) was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD55 Antibody Target Details CD55 Application Details ProductDetails: References for anti-CD55 antibody (ABIN118737) Images back to top
|Buffer||PBS, pH 7.4, with 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ProductDetails: References for anti-CD55 antibody (ABIN118737)Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling Images back to top
|Product cited in:||
Wenzel, Zabojszcza, Carl, Taubert, Lass, Harris, Ho, Schulz, Hummel, Hubner, Osterziel, Spuler: "Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6219-25, 2005
Holmes, Simpson, Wainwright, Tate, Houlihan, Sawyer, Rogers, Spring, Anstee, Tanner: "Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 8, pp. 3099-105, 1990
Lublin, Atkinson: "Decay-accelerating factor: biochemistry, molecular biology, and function." in: Annual review of immunology, Vol. 7, pp. 35-58, 1989
Daniels: "Cromer-related antigens--blood group determinants on decay-accelerating factor." in: Vox sanguinis, Vol. 56, Issue 4, pp. 205-11, 1989
ImagesProduct Details anti-CD55 Antibody Target Details CD55 Application Details Handling ProductDetails: References for anti-CD55 antibody (ABIN118737) back to top