anti-Human CD55 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • Daf
  • Daf-GPI
  • Daf1
  • GPI-DAF
  • CR
  • CROM
  • DAF
  • TC
  • AI323748
  • Cd55b
  • Daf-TM
  • TM-DAF
  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • decay accelerating factor 2
  • Cd55
  • CD55
  • Daf2
Reactivity
Human
412
56
22
9
9
4
3
2
2
2
Host
Mouse
338
97
24
8
7
3
3
Clonality
Monoclonal
Conjugate
This CD55 antibody is un-conjugated
53
47
30
13
11
11
10
9
8
8
6
6
6
6
6
6
5
5
5
4
4
4
3
3
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
359
186
97
64
57
53
42
39
12
8
6
6
6
5
2
2
1
1
1
1
1
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Catalog No. ABIN118737
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118738 FACS IHC (fro) Mouse IgG1 Log in to see 67 4
1 ABIN2480958 IHC (fro) FACS WB Mouse IgG1 Log in to see 67 3
1 ABIN2144924 IHC (fro) FACS WB PE Mouse IgG1 Log in to see 4A143-30 2
1 ABIN2144926 IHC (fro) FACS WB PE Mouse IgG1 Log in to see 4A143-30 2
1 ABIN2144640 IHC (fro) FACS WB FITC Mouse IgG1 Log in to see 4A143-30 2
1 ABIN2144643 IHC (fro) FACS WB FITC Mouse IgG1 Log in to see 4A143-30 2
1 ABIN319782 FACS IHC (fro) Mouse IgG1 Log in to see 67 4
1 ABIN1838119 IHC (fro) FACS Mouse IgG1 Log in to see 67
1 ABIN600851 IHC (fro) FACS IHC (p) Mouse IgG1 Log in to see 67
1 ABIN203999 FACS IHC (fro) Mouse IgG1 Log in to see
1 ABIN211859 FACS IHC (fro) Mouse IgG1 Log in to see
1 ABIN198278 FACS IHC (fro) Mouse IgG1 Log in to see 67
1 ABIN2689337 IHC (fro) FACS Mouse IgG2a, kappa Log in to see IA10 1
1 ABIN2593515 IHC (fro) FACS FITC Mouse IgG1 Log in to see 143-30
1 ABIN2593517 IHC (fro) FACS BIMA Mouse IgG1 Log in to see 143-30
1 ABIN4294844 FACS IF IHC (fro) Mouse IgG1 kappa Log in to see 143-30
1 ABIN4294843 FACS IF IHC (fro) Mouse IgG1 kappa Log in to see 143-30
1 ABIN4294845 FACS IF IHC (fro) Mouse IgG1 kappa Log in to see 143-30
1 ABIN371558 FACS IHC (fro) Mouse IgG1 Log in to see 143-30 2
1 ABIN600850 FACS IHC (fro) Mouse IgG1 Log in to see

General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Reactivity Human
(412), (56), (22), (9), (9), (4), (3), (2), (2), (2)
Host Mouse
(338), (97), (24), (8), (7), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is un-conjugated
(53), (47), (30), (13), (11), (11), (10), (9), (8), (8), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(359), (186), (97), (64), (57), (53), (42), (39), (12), (8), (6), (6), (6), (5), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling ProductDetails: References for anti-CD55 antibody (ABIN118737) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G.
Immunogen K562 cells
Clone 67
Isotype IgG1

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling ProductDetails: References for anti-CD55 antibody (ABIN118737) Images back to top
Antigen
Alternative Name CD55 / DAF (CD55 Antibody Abstract)
Background CD55 is the complement regulatory protein, decay accelerating factor (DAF). It is a 70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin. CD55 is expressed by a wide range of cell types.Synonyms: Complement decay-accelerating factor
Gene ID 9606
UniProt P08174
Pathways Complement System, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling ProductDetails: References for anti-CD55 antibody (ABIN118737) Images back to top
Application Notes Flow Cytometry: Use 10 μL of 1/10-1/25 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections: 1/100-1/1000. Recommended Positive Control Tissue: Human Tonsil. (Successful use in immunohistochemistry on paraffin embedded sections (1/50-1/100using heat treatment for antigen retrieval) was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details ProductDetails: References for anti-CD55 antibody (ABIN118737) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, with 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD55 antibody (ABIN118737)

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling Images back to top
Product cited in:

Wenzel, Zabojszcza, Carl, Taubert, Lass, Harris, Ho, Schulz, Hummel, Hubner, Osterziel, Spuler: "Increased susceptibility to complement attack due to down-regulation of decay-accelerating factor/CD55 in dysferlin-deficient muscular dystrophy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 9, pp. 6219-25, 2005

Background publications

Holmes, Simpson, Wainwright, Tate, Houlihan, Sawyer, Rogers, Spring, Anstee, Tanner: "Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 8, pp. 3099-105, 1990

Lublin, Atkinson: "Decay-accelerating factor: biochemistry, molecular biology, and function." in: Annual review of immunology, Vol. 7, pp. 35-58, 1989

Daniels: "Cromer-related antigens--blood group determinants on decay-accelerating factor." in: Vox sanguinis, Vol. 56, Issue 4, pp. 205-11, 1989

Images

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling ProductDetails: References for anti-CD55 antibody (ABIN118737) back to top
Supplier Images
 image for anti-Complement Decay-Accelerating Factor (CD55) antibody (ABIN118737) Staining of human peripheral blood monocytes with Mouse anti Human CD55 (ABIN118737).