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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||11 Antibodies||49 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||32 Antibodies||322 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||11 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||54 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Western Blotting (WB)||15 Antibodies|
|Functional Studies (Func)||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||79 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Antigen||Complement Decay-Accelerating Factor (CD55) Antibodies|
|Epitope||AA 35-347 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This CD55 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CD55 AntibodyTarget Details CD55 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Complement decay-accelerating factor(CD55) detection. Tested with WB,IHC-P in Human,Mouse.
Gene Name: CD55 Molecule, decay accelerating factor for complement (Cromer blood group)
Protein Name: Complement decay-accelerating factor
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human CD55 recombinant protein (Position: D35-K347). Human CD55 shares 49.1% amino acid (aa) sequence identity with mouse CD55.|
Target Details CD55Product Details anti-CD55 Antibody Application Details Handling Images back to top
|Alternative Name||CD55 (CD55 Antibody Abstract)|
|Background||Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.|
|Research Area||Immunology, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD55 Antibody Target Details CD55 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD55 Antibody Target Details CD55 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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