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|Application / Reactivity||Non-Human Primate||Human|
|Flow Cytometry (FACS)||4 Antibodies||201 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies||24 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||38 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Functional Studies (Func)||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies|
|Immunofluorescence (IF)||41 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies|
|Immunohistochemistry (IHC)||61 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||29 Antibodies|
|Intracellular Flow Cytometry (ICFC)||5 Antibodies|
|Antigen||Complement Decay-Accelerating Factor (CD55) Antibodies|
|Epitope||Domain SCR4 Alternatives|
|Reactivity||Human, Non-Human Primate Alternatives|
|Conjugate||This CD55 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
|6 references available|
|Supplier||Log in to see|
Product Details anti-CD55 AntibodyTarget Details CD55 Application Details Handling References for anti-CD55 Antibody (ABIN94183) Images
|Specificity||The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells, it is weakly present on NK cells.HLDA V, WS Code AS S016|
|Purification||Purified from ascites by precipitation methods and size-exclusion chromatography.|
|Purity||> 95 % (by SDS-PAGE)|
|Immunogen||HPB-ALL human T cell line|
Target Details CD55Product Details anti-CD55 Antibody Application Details Handling References for anti-CD55 Antibody (ABIN94183) Images back to top
|Alternative Name||CD55 (CD55 Antibody Abstract)|
|Background||CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumours, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.|
|Research Area||Immunology, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD55 Antibody Target Details CD55 Handling References for anti-CD55 Antibody (ABIN94183) Images back to top
Flow Cytometry: Recommended dilution: 10 µg/mL
Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/mL
Working concentrations should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD55 Antibody Target Details CD55 Application Details References for anti-CD55 Antibody (ABIN94183) Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-CD55 Antibody (ABIN94183)Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling Images back to top
|Product cited in:||
Angelisová, Drbal, Horejsí et al.: "Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol-anchored proteins and Lyn kinase." in: Blood, Vol. 93, Issue 4, pp. 1437-9, 1999 (PubMed).
VanLandingham, Cekic, Cutler et al.: "Neurosteroids reduce inflammation after TBI through CD55 induction." in: Neuroscience letters, Vol. 425, Issue 2, pp. 94-8, 2007 (PubMed).
Abbott, Spendlove, Roversi et al.: "Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55." in: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 22023-32, 2007 (PubMed).
Miwa, Maldonado, Zhou et al.: "Decay-accelerating factor ameliorates systemic autoimmune disease in MRL/lpr mice via both complement-dependent and -independent mechanisms." in: The American journal of pathology, Vol. 170, Issue 4, pp. 1258-66, 2007 (PubMed).
Mikesch, Buerger, Simon et al.: "Decay-accelerating factor (CD55): a versatile acting molecule in human malignancies." in: Biochimica et biophysica acta, Vol. 1766, Issue 1, pp. 42-52, 2006 (PubMed).
Miwa, Maldonado, Zhou et al.: "Deletion of decay-accelerating factor (CD55) exacerbates autoimmune disease development in MRL/lpr mice." in: The American journal of pathology, Vol. 161, Issue 3, pp. 1077-86, 2002 (PubMed).
ImagesProduct Details anti-CD55 Antibody Target Details CD55 Application Details Handling References for anti-CD55 Antibody (ABIN94183) back to top