anti-Human CD59 antibody for Western Blotting

Recommended CD59 Antibody (supplied by: Log in to see )

Antigen
CD59 Antibodies
  • 16.3A5
  • 1F5
  • EJ16
  • EJ30
  • EL32
  • G344
  • HRF-20
  • HRF20
  • MAC-IP
  • MACIF
  • MEM43
  • MIC11
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • MSK21
  • p18-20
  • AA987121
  • Cd59
  • protectin
  • Cd59a
  • Cd59b
  • MACIP
  • CD59 molecule (CD59 blood group)
  • CD59a antigen
  • CD59 molecule
  • CD59
  • Cd59a
  • Cd59
Reactivity
Human, Mouse (Murine)
510
43
37
23
17
14
9
8
2
2
1
1
Host
Mouse
408
125
41
7
1
Clonality
Monoclonal
Conjugate
This CD59 antibody is un-conjugated
63
53
44
22
14
12
12
12
10
10
10
10
10
10
10
6
6
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
423
207
192
129
113
90
82
47
45
25
13
8
8
4
3
2
1
1
1
1
1
1
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Catalog No. ABIN269032
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0849469 ABIN1077443 ELISA WB Rabbit IgG N-Term Log in to see
3.0849469 ABIN1098134 ICC FACS IHC ELISA WB Mouse IgG1 AA 31-111 Log in to see 8D2B8 2
3.0849469 ABIN1999739 IP ELISA WB Rabbit IgG AA 1-101 Log in to see Polyclonal 4
3.0849469 ABIN4294896 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.0849469 ABIN94203 FACS IHC (p) IP WB Mouse IgG2b Log in to see MEM-43-5 7
3.0849469 ABIN3187782 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0849469 ABIN3197978 IHC WB Rabbit Log in to see Polyclonal
3.0849469 ABIN1871644 IHC WB Rabbit IgG Log in to see Polyclonal
3.0849469 ABIN4294895 CyTOF ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 8D2B8
3.0849469 ABIN3022399 IHC WB Rabbit IgG Log in to see Polyclonal
3.0849469 ABIN4294964 WB Mouse IgG1 Log in to see 2E11
3.0849469 ABIN1174497 IHC ELISA WB Biotin Rabbit IgG AA 26-102 Log in to see Polyclonal
3.0849469 ABIN947663 ELISA WB Mouse IgG2a kappa AA 1-128, full length Log in to see 3G6
3.0849469 ABIN1174495 ICC IHC IP WB Rabbit IgG AA 26-102 Log in to see Polyclonal
3.0849469 ABIN2434430 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0849469 ABIN514216 WB Mouse AA 1-128, full length Log in to see Polyclonal
3.0849469 ABIN514218 IF WB Rabbit AA 1-128, full length Log in to see Polyclonal
3.0849469 ABIN1774798 WB Mouse AA 1-128, full length Log in to see Polyclonal
3.0849469 ABIN2404518 IHC WB Rabbit IgG Log in to see Polyclonal
3.0849469 ABIN1845905 ELISA FACS IF/ICC IHC WB Mouse IgG1 AA 31-111 Log in to see

General

Antigen CD59 Antibodies
Reactivity Human, Mouse (Murine)
(510), (43), (37), (23), (17), (14), (9), (8), (2), (2), (1), (1)
Host Mouse
(408), (125), (41), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD59 antibody is un-conjugated
(63), (53), (44), (22), (14), (12), (12), (12), (10), (10), (10), (10), (10), (10), (10), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(423), (207), (192), (129), (113), (90), (82), (47), (45), (25), (13), (8), (8), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD59 Antibody

Target Details CD59 Application Details Handling References for anti-CD59 antibody (ABIN269032) Images
Specificity The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells, it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies. HLDA V, WS Code AS S012
Purification Protein A purified
Immunogen Thymocytes and T lymphocytes.
Clone MEM-43-5
Isotype IgG2b

Target Details CD59

Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 antibody (ABIN269032) Images back to top
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background Gene Symbol: CD59
Gene ID 966
Research Area Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 antibody (ABIN269032) Images back to top
Application Notes Western Blot 1 - 2 μg/mL, Flow Cytometry 1 μg/mL, Immunohistochemistry, Immunoprecipitation 1:50, Immunohistochemistry-Paraffin 5 μg/mL, CyTOF-readyWestern Blot - Non-reducing conditions.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 antibody (ABIN269032) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-CD59 antibody (ABIN269032)

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Product cited in:

Yuan, Li, Baines, Gavriilaki, Ye, Wen, Braunstein, Biesecker, Cheng, Dong, Brodsky: "A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model." in: PLoS ONE, Vol. 12, Issue 4, pp. e0174074, 2017 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Su, Wang, Gao, Liu, Pan, Hu, Beyer, Shi, Zhou, Zhang, Serra, Wüthrich, Mei: "Excessive activation of the alternative complement pathway in autosomal dominant polycystic kidney disease." in: Journal of internal medicine, Vol. 276, Issue 5, pp. 470-85, 2014 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling References for anti-CD59 antibody (ABIN269032) back to top
Supplier Images
Western Blotting (WB) image for anti-CD59 (CD59) antibody (ABIN269032) Western Blot: CD59 Antibody (MEM-43/5) [ABIN269032] - Fig. 1. Western Blotting analys...