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anti-Human CD59 antibody for Western Blotting

Recommended CD59 Antibody (supplied by: Log in to see )

Antigen
CD59 Antibodies
  • 1F5
  • 16.3A5
  • AA987121
  • Cd59
  • Cd59a
  • Cd59b
  • EJ16
  • EJ30
  • EL32
  • G344
  • HRF-20
  • HRF20
  • MAC-IP
  • MACIF
  • MACIP
  • MEM43
  • MIC11
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • MSK21
  • p18-20
  • protectin
Epitope
AA 31-111
13
7
7
7
7
6
5
5
5
4
3
2
2
1
1
1
Reactivity
Human
340
22
19
10
9
7
2
2
2
1
1
Host
Mouse
248
109
25
7
1
Clonality
Monoclonal
Conjugate
This CD59 antibody is un-conjugated
57
48
38
10
9
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
244
137
95
77
76
70
31
29
17
13
11
6
5
3
2
2
1
Supplier
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Catalog No. ABIN1098134
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN94203 FACS IHC (p) IP WB Mouse IgG2b Log in to see MEM-43-5 7
0.12303223 ABIN1077443 ELISA WB Rabbit IgG N-Term Log in to see
0.12303223 ABIN1999739 IP ELISA WB Rabbit IgG AA 1-101 Log in to see Polyclonal 4
0.12303223 ABIN1871644 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN1174497 IHC ELISA WB Biotin Rabbit IgG AA 26-102 Log in to see Polyclonal
0.12303223 ABIN3022399 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN947663 ELISA WB Mouse IgG2a kappa AA 1-128, full length Log in to see 3G6
0.12303223 ABIN3187782 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12303223 ABIN1174495 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 26-102 Log in to see Polyclonal
0.12303223 ABIN514216 WB Mouse AA 1-128, full length Log in to see Polyclonal
0.12303223 ABIN514218 IF WB Rabbit AA 1-128, full length Log in to see Polyclonal
0.12303223 ABIN1774798 WB Mouse AA 1-128, full length Log in to see Polyclonal
0.12303223 ABIN1845905 ICC FACS IF IHC ELISA WB Mouse IgG1 AA 31-111 Log in to see
0.12303223 ABIN2434430 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2404518 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN1774799 WB Mouse AA 1-128, full length Log in to see Polyclonal
0.0011806369 ABIN119472 FACS IHC (fro) IHC (p) WB Rat IgG2b Log in to see YTH53-1 6
0.0011806369 ABIN118747 EIA FACS IHC (fro) IHC (p) IP WB Mouse IgG2a Log in to see MEM-43 4
0.0011806369 ABIN118751 Func FACS IHC (fro) IHC (p) WB Mouse IgG2a Log in to see MEM-43 2
0.0011806369 ABIN320004 FACS IHC (fro) WB Rat IgG2b Log in to see YTH53-1 4

Top referenced anti-CD59 antibody for Western Blotting

Similar anti-CD59 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 68 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 241 Antibodies
Functional Studies (Func) 18 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 32 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 75 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies
Immunoprecipitation (IP) 68 Antibodies

General

Antigen CD59 Antibodies
Epitope AA 31-111
(13), (7), (7), (7), (7), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1)
Reactivity Human
(340), (22), (19), (10), (9), (7), (2), (2), (2), (1), (1)
Host Mouse
(248), (109), (25), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD59 antibody is un-conjugated
(57), (48), (38), (10), (9), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(244), (137), (95), (77), (76), (70), (31), (29), (17), (13), (11), (6), (5), (3), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-CD59 Antibody

Target Details CD59 Application Details Handling References for anti-CD59 Antibody (ABIN1098134) Images
Purification Purified
Immunogen Purified recombinant fragment of human CD59 (AA 31-111) expressed in E. coli.
Clone 8D2B8
Isotype IgG1

Target Details CD59

Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 Antibody (ABIN1098134) Images back to top
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. , ,
Molecular Weight 14.2 kDa
Gene ID 966
HGNC 966
Research Area Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 Antibody (ABIN1098134) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/50 -1/200, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 Antibody (ABIN1098134) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD59 Antibody (ABIN1098134)

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Product cited in:

Alegretti, Mucenic, Merzoni, Faulhaber, Silla, Xavier: "Expression of CD55 and CD59 on peripheral blood cells from systemic lupus erythematosus (SLE) patients." in: Cellular immunology, Vol. 265, Issue 2, pp. 127-32, 2010

Ma, Chang, Qin, Sun, Huang, He: "Decreased expression of complement regulatory proteins, CD55 and CD59, on peripheral blood leucocytes in patients with type 2 diabetes and macrovascular diseases." in: Chinese medical journal, Vol. 122, Issue 18, pp. 2123-8, 2009

Images

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling References for anti-CD59 Antibody (ABIN1098134) back to top
Supplier Images
ELISA image for anti-CD59 antibody (CD59) (AA 31-111) (ABIN1098134) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-CD59 antibody (CD59) (AA 31-111) (ABIN1098134) Figure 2: Flow cytometric analysis of HeLa cells using CD59 mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-CD59 antibody (CD59) (AA 31-111) (ABIN1098134) Figure 3: Immunofluorescence analysis of Hela cells using CD59 mouse mAb (green) . Bl...
Immunocytochemistry (ICC) image for anti-CD59 antibody (CD59) (AA 31-111) (ABIN1098134) Figure 4: Immunofluorescence analysis of MCF-7 cells using CD59 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-CD59 antibody (CD59) (AA 31-111) (ABIN1098134) Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues us...
Immunohistochemistry (IHC) image for anti-CD59 antibody (CD59) (AA 31-111) (ABIN1098134) Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues...
Western Blotting (WB) image for anti-CD59 antibody (CD59) (AA 31-111) (ABIN1098134) Figure 7: Western blot analysis using CD59 mAb against human CD59 recombinant protein...
Western Blotting (WB) image for anti-CD59 antibody (CD59) (AA 31-111) (ABIN1098134) Figure 8: Western blot analysis using CD59 mAb against HEK293 (1) and CD59 (AA: 31-11...