anti-Human CR1 antibody for Immunocytochemistry

Recommended CR1 Antibody (supplied by: Log in to see )

Antigen
Complement Component Receptor 1 (CD35) (CR1) Antibodies
  • C3BR
  • C4BR
  • CD35
  • KN
  • C3DR
  • CD21
  • Cr-1
  • Cr-2
  • Cr1
  • complement component (3b/4b) receptor 1 (Knops blood group)
  • complement component (3d/Epstein Barr virus) receptor 2
  • complement receptor 2
  • complement receptor type 1-like
  • CR1
  • CR2
  • Cr2
  • LOC100514723
Reactivity
Human, Primate
302
70
51
46
46
17
15
3
2
Host
Mouse
266
78
2
Clonality
Monoclonal
Conjugate
This CR1 antibody is un-conjugated
35
34
15
9
9
8
8
7
7
6
6
6
6
6
6
6
6
4
3
3
3
2
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
259
159
124
105
67
66
49
46
39
13
9
9
6
2
2
2
1
1
1
1
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Catalog No. ABIN4292473
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4292471 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E11
1 ABIN4292472 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E11
1 ABIN4292490 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see E11
1 ABIN2749062 IHC (f) ICC FACS IP IHC (p) WB Mouse IgG1 Log in to see E11 2
1 ABIN2749129 IHC (f) ICC FACS IP IHC (p) WB PE Mouse IgG1 Log in to see E11 3
1 ABIN2749128 IHC (f) ICC FACS IP IHC (p) WB FITC Mouse IgG1 Log in to see E11 2
1 ABIN2619499 ICC IHC (fro) FACS WB Biotin Mouse IgG1 Log in to see To5
1 ABIN2596447 FACS ICC IHC (fro) WB APC Mouse IgG1 Log in to see To5
1 ABIN2144575 IHC (f) ICC FACS IP IHC (p) WB FITC Mouse IgG1 Log in to see 4AE11
1 ABIN2144864 IHC (f) ICC FACS IP IHC (p) WB PE Mouse IgG1 Log in to see 4AE11
1 ABIN2144862 IHC (f) ICC FACS IP IHC (p) WB PE Mouse IgG1 Log in to see 4AE11
1 ABIN1959969 ICC IHC (fro) FACS IP IHC (p) WB Mouse IgG1 Log in to see E11
1 ABIN2144577 IHC (f) ICC FACS IP IHC (p) WB FITC Mouse IgG1 Log in to see 4AE11
1 ABIN4292474 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM554
1 ABIN4292475 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM554
1 ABIN4292476 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see SPM554
1 ABIN4292511 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see SPM554
1 ABIN4292506 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM554
1 ABIN4292513 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see SPM554
1 ABIN4292505 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see SPM554

Top referenced anti-CR1 antibody for Immunocytochemistry

Similar anti-CR1 Antibodies

Application / Reactivity Primate Human
Cytometry by Time of Flight (CyTOF) 2 Antibodies 5 Antibodies
ELISA 36 Antibodies 47 Antibodies
Flow Cytometry (FACS) 30 Antibodies 222 Antibodies
Immunocytochemistry (ICC) 26 Antibodies 40 Antibodies
Immunofluorescence (IF) 25 Antibodies 50 Antibodies
Immunohistochemistry (IHC) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 26 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Western Blotting (WB) 26 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunoassay (IA) 2 Antibodies

General

Antigen Complement Component Receptor 1 (CD35) (CR1) Antibodies
Reactivity Human, Primate
(302), (70), (51), (46), (46), (17), (15), (3), (2)
Host Mouse
(266), (78), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CR1 antibody is un-conjugated
(35), (34), (15), (9), (9), (8), (8), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(259), (159), (124), (105), (67), (66), (49), (46), (39), (13), (9), (9), (6), (2), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CR1 Antibody

Target Details CR1 Application Details Handling ProductDetails: References for anti-CR1 antibody (ABIN4292473) Images
Specificity Recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.
Purification Protein G purified
Immunogen Intact human monocytes were used as immunogen to generate the CD35 antibody (Hogg et al, 1984).
Clone E11
Isotype IgG1 kappa

Target Details CR1

Product Details anti-CR1 Antibody Application Details Handling ProductDetails: References for anti-CR1 antibody (ABIN4292473) Images back to top
Antigen
Alternative Name CD35 (CR1 Antibody Abstract)
Background Gene Symbol: CR1
Molecular Weight Theoretical MW: 215 kDa
Gene ID 1378
UniProt P17927
Research Area Surface Receptors of Immune Cells, CD Antigens
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-CR1 Antibody Target Details CR1 Handling ProductDetails: References for anti-CR1 antibody (ABIN4292473) Images back to top
Application Notes Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mLHuman Chromosome Location: 1q32.2 Mol. Weight of Antigen: 210-220 kDa Use in Western Blot has been reported in literature. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CR1 Antibody Target Details CR1 Application Details ProductDetails: References for anti-CR1 antibody (ABIN4292473) Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.

ProductDetails: References for anti-CR1 antibody (ABIN4292473)

Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling Images back to top
Product cited in:

Zhang, Patel, Dey, Ghorani, Rai, Elham, Castleton, Fielding: "Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 3, pp. 1002-10, 2012

Mokuda, Doi, Takasugi: "Simultaneous quantitative analysis of the expression of CD64 and CD35 on neutrophils as markers to differentiate between bacterial and viral infections in patients with rheumatoid arthritis." in: Modern rheumatology, Vol. 22, Issue 5, pp. 750-7, 2012

Cho, Kim, Kim, Ryu, Woo: "Tonsil-derived mesenchymal progenitor cells acquire a follicular dendritic cell phenotype under cytokine stimulation." in: Cytokine, Vol. 59, Issue 2, pp. 211-4, 2012

Bagdi, Krenacs, Krenacs, Miller, Isaacson et al.: "Follicular dendritic cells in reactive and neoplastic lymphoid tissues: a reevaluation of staining patterns of CD21, CD23, and CD35 antibodies in paraffin sections after wet heat-induced epitope ..." in: Applied immunohistochemistry & molecular morphology : AIMM, Vol. 9, Issue 2, pp. 117-24, 2001

Images

Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling ProductDetails: References for anti-CR1 antibody (ABIN4292473) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN4292473) Immunohistochemistry-Paraffin: CD35 Antibody (E11) [NBP2-29460] - Formalin-fixed, par...