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anti-Human CR1 antibody for Western Blotting

Recommended CR1 Antibody (supplied by: Log in to see )

Antigen
Complement Component Receptor 1 (CD35) (CR1) Antibodies
  • C3BR
  • C3DR
  • C4BR
  • CD21
  • CD35
  • Cr-1
  • Cr-2
  • Cr1
  • KN
Reactivity
Human
193
17
14
10
10
6
5
2
Host
Rabbit
168
27
2
Clonality
Monoclonal
Conjugate
This CR1 antibody is un-conjugated
33
30
10
3
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
149
75
59
42
42
38
21
20
11
11
11
4
4
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1689163
$ 648.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011728628 ABIN118609 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 4
0.0011728628 ABIN118608 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 4
0.0011728628 ABIN319720 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 2
0.0011728628 ABIN501189 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 2
0.0011728628 ABIN118606 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 4
0.0011728628 ABIN1106316 EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 31R 1
0.0011728628 ABIN962071 IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see E11
0.0011728628 ABIN610051 FACS WB Biotin Mouse IgG1 Log in to see E11
0.0011728628 ABIN610052 FACS WB FITC Mouse IgG1 Log in to see E11
0.0011728628 ABIN610053 FACS WB PE Mouse IgG1 Log in to see E11
0.0011728628 ABIN2225643 FACS IF IP IHC ELISA WB Mouse IgG1 kappa Log in to see E11
0.0011728628 ABIN135765 FACS IHC (p) IP IHC WB Mouse IgG1 Log in to see E11 2
0.0011728628 ABIN2749062 IHC (f) ICC FACS IP IHC (p) WB Mouse IgG1 Log in to see E11 2
0.0011728628 ABIN1723986 IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see E11
0.0011728628 ABIN232371 FACS IF IP IHC WB Mouse IgG1 Log in to see
0.0011728628 ABIN2749128 IHC (f) ICC FACS IP IHC (p) WB FITC Mouse IgG1 Log in to see E11 2
0.0011728628 ABIN2749129 IHC (f) ICC FACS IP IHC (p) WB PE Mouse IgG1 Log in to see E11 3
0.0011728628 ABIN609303 IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see
0.0011728628 ABIN609302 IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see
0.0011728628 ABIN961127 IHC (fro) FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see 31R

Top referenced anti-CR1 antibody for Western Blotting

Similar anti-CR1 Antibodies

Application / Reactivity Human
ELISA 11 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 147 Antibodies
Functional Studies (Func) 1 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 41 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 60 Antibodies
Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) 1 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies

General

Antigen Complement Component Receptor 1 (CD35) (CR1) Antibodies
Reactivity Human
(193), (17), (14), (10), (10), (6), (5), (2)
Host Rabbit
(168), (27), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CR1 antibody is un-conjugated
(33), (30), (10), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(149), (75), (59), (42), (42), (38), (21), (20), (11), (11), (11), (4), (4), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CR1 Antibody

Target Details CR1 Application Details Handling Images
Characteristics Cellular Localization: Membrane
Purification Purified by Protein A/G
Immunogen Synthetic peptide derived from human CD35 protein.
Clone SP197
Isotype IgG

Target Details CR1

Product Details anti-CR1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD35 (CR1 Antibody Abstract)
Background CD35, also known as complement receptor type 1, is a monomeric single-pass type 1 membrane glycoprotein from complement activation (RCA) family. CD35 binds complement components C3b and C4b and has an inhibitory effect on complement activation by both the classical and alternative pathways. CD35 is expressed by glomerular podocytes, follicular dendritic cells, erythrocytes, and some leukocytes. Abnormal expression of this protein has been associated with follicular dendritic cell tumor, sarcoidosis, gallbladder carcinomas, mesangiocapillary glomerulonephritis, and systemic lupus erythematosus.
Molecular Weight 280 kDa
Gene ID 1378
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CR1 Antibody Target Details CR1 Handling Images back to top
Application Notes IHC Dilution: 1:100
Comment

Positive Control:
IHC (p): Kidney, Tonsil
WB: Human Tonsil Lysate

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only

Handling

Product Details anti-CR1 Antibody Target Details CR1 Application Details Images back to top
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

Images

Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody
Immunohistochemistry (IHC) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody (Image 5)