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|Application / Reactivity||Human|
|Cytometry by Time of Flight (CyTOF)||5 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||216 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunoassay (IA)||2 Antibodies|
|Immunocytochemistry (ICC)||40 Antibodies|
|Immunofluorescence (IF)||48 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||68 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||9 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||68 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||115 Antibodies|
|Antigen||Complement Component Receptor 1 (CD35) (CR1) Antibodies|
|Conjugate||This CR1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CR1 AntibodyTarget Details CR1 Application Details Handling Images
|Characteristics||Cellular Localization: Membrane|
|Purification||Purified by Protein A/G|
|Immunogen||Synthetic peptide derived from human CD35 protein.|
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|Alternative Name||CD35 (CR1 Antibody Abstract)|
|Background||CD35, also known as complement receptor type 1, is a monomeric single-pass type 1 membrane glycoprotein from complement activation (RCA) family. CD35 binds complement components C3b and C4b and has an inhibitory effect on complement activation by both the classical and alternative pathways. CD35 is expressed by glomerular podocytes, follicular dendritic cells, erythrocytes, and some leukocytes. Abnormal expression of this protein has been associated with follicular dendritic cell tumor, sarcoidosis, gallbladder carcinomas, mesangiocapillary glomerulonephritis, and systemic lupus erythematosus.|
|Molecular Weight||280 kDa|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity|
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|Application Notes||IHC Dilution: 1:100|
IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
|Restrictions||For Research Use only|
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|Buffer||rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.|
|Precaution of Use||
1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
|Handling Advice||Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.|
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