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anti-Human CR1 antibody for Western Blotting

Recommended CR1 Antibody (supplied by: Log in to see )

Antigen
Complement Component Receptor 1 (CD35) (CR1) Antibodies
  • C3BR
  • C4BR
  • CD35
  • KN
  • C3DR
  • CD21
  • Cr-1
  • Cr-2
  • Cr1
  • complement component (3b/4b) receptor 1 (Knops blood group)
  • complement component (3d/Epstein Barr virus) receptor 2
  • complement receptor 2
  • complement receptor type 1-like
  • CR1
  • CR2
  • Cr2
  • LOC100514723
Reactivity
Human
291
70
51
47
46
17
15
3
2
Host
Rabbit
259
74
2
Clonality
Monoclonal
Conjugate
This CR1 antibody is un-conjugated
34
33
15
9
9
8
8
7
7
6
6
6
6
6
6
6
6
4
3
3
3
2
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
254
154
116
105
68
67
48
47
40
13
9
9
6
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN1689163
$ 661.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN3222849 WB Rabbit Log in to see Polyclonal
3.0726016 ABIN4903370 WB Rabbit IgG Log in to see Polyclonal
3.0726016 ABIN4292463 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN118609 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 4
1 ABIN118608 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 4
1 ABIN4292473 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see E11 4
1 ABIN118606 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 4
1 ABIN319720 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 2
1 ABIN501189 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 2
1 ABIN1106316 EIA IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 31R 1
1 ABIN962071 IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see E11
1 ABIN337278 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11
1 ABIN135765 FACS IHC (p) IP IHC WB Mouse IgG1 Log in to see E11 2
1 ABIN1723986 IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see E11
1 ABIN2225643 FACS IF IP IHC ELISA WB Mouse IgG1 kappa Log in to see E11
1 ABIN610053 FACS WB PE Mouse IgG1 Log in to see E11
1 ABIN576542 FACS IHC (fro) IHC (p) WB PE Mouse IgG1 Log in to see E11
1 ABIN610051 FACS WB Biotin Mouse IgG1 Log in to see E11
1 ABIN610052 FACS WB FITC Mouse IgG1 Log in to see E11
1 ABIN2749128 IHC (f) ICC FACS IP IHC (p) WB FITC Mouse IgG1 Log in to see E11 2

Top referenced anti-CR1 antibody for Western Blotting

Similar anti-CR1 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 5 Antibodies
ELISA 47 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 216 Antibodies
Functional Studies (Func) 1 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 40 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 68 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 68 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 115 Antibodies

General

Antigen Complement Component Receptor 1 (CD35) (CR1) Antibodies
Reactivity Human
(291), (70), (51), (47), (46), (17), (15), (3), (2)
Host Rabbit
(259), (74), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CR1 antibody is un-conjugated
(34), (33), (15), (9), (9), (8), (8), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(254), (154), (116), (105), (68), (67), (48), (47), (40), (13), (9), (9), (6), (2), (2), (2), (1), (1), (1), (1)
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Product Details anti-CR1 Antibody

Target Details CR1 Application Details Handling Images
Characteristics Cellular Localization: Membrane
Purification Purified by Protein A/G
Immunogen Synthetic peptide derived from human CD35 protein.
Clone SP197
Isotype IgG

Target Details CR1

Product Details anti-CR1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD35 (CR1 Antibody Abstract)
Background CD35, also known as complement receptor type 1, is a monomeric single-pass type 1 membrane glycoprotein from complement activation (RCA) family. CD35 binds complement components C3b and C4b and has an inhibitory effect on complement activation by both the classical and alternative pathways. CD35 is expressed by glomerular podocytes, follicular dendritic cells, erythrocytes, and some leukocytes. Abnormal expression of this protein has been associated with follicular dendritic cell tumor, sarcoidosis, gallbladder carcinomas, mesangiocapillary glomerulonephritis, and systemic lupus erythematosus.
Molecular Weight 280 kDa
Gene ID 1378
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-CR1 Antibody Target Details CR1 Handling Images back to top
Application Notes IHC Dilution: 1:100
Comment

Positive Control:
IHC (p): Kidney, Tonsil
WB: Human Tonsil Lysate

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only

Handling

Product Details anti-CR1 Antibody Target Details CR1 Application Details Images back to top
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

Images

Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody
Immunohistochemistry (IHC) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN1689163) anti-Complement Component Receptor 1 (CD35) (CR1) antibody (Image 5)