PSMD14 antibody (Center)
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- Target See all PSMD14 Antibodies
- PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))
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Binding Specificity
- Center
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PSMD14 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Characteristics
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Rabbit Polyclonal antibody to MAD1 (MAD1 mitotic arrest deficient-like 1 (yeast))
MAD1 antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.
- Isotype
- IgG
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- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
- Comment
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Positive Control: 293T , A431 , HeLa , HepG2 , A375
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling." in: The Journal of cell biology, Vol. 218, Issue 4, pp. 1108-1117, (2020) (PubMed).
: "
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MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling." in: The Journal of cell biology, Vol. 218, Issue 4, pp. 1108-1117, (2020) (PubMed).
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- Target
- PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))
- Alternative Name
- MAD1 (PSMD14 Products)
- Synonyms
- PAD1 antibody, POH1 antibody, RPN11 antibody, 2610312C03Rik antibody, 3200001M20Rik antibody, AA986732 antibody, Pad1 antibody, Poh1 antibody, rpm11 antibody, zgc:162272 antibody, proteasome 26S subunit, non-ATPase 14 antibody, proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody, 26S proteasome non-ATPase regulatory subunit 14 antibody, proteasome 26S subunit, non-ATPase 14 L homeolog antibody, 26S proteasome-associated pad1 homolog antibody, PSMD14 antibody, Psmd14 antibody, rpn-11 antibody, psmd14 antibody, psmd14.L antibody
- Background
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MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.
Cellular Localization: Nucleus , Kinetochore - Molecular Weight
- 83 kDa
- Gene ID
- 8379
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication, M Phase, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Ubiquitin Proteasome Pathway
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