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SQSTM1 antibody (AA 51-150)

Name: SQSTM1 antibody (AA 51-150)
SKU: ABIN682153
Price: 384.62 USD
Availability: InStock

SQSTM1 Reactivity: Human, Mouse, Rat, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

HeLa cell lysates probed with Anti-SQSTM1 Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

ELISA

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SQSTM1 Polyclonal Antibody, Unconjugated (ABIN682153) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

L1 rat kidney lysates, L2 rat brain lysates probed : Anti-GDF9 (ABIN682153) at 1:200 in 4 °C.

SDS

L1 rat kidney lysates, L2 rat brain lysates probed : Anti-GDF9 at 1:200 in 4˚C.

CQ inhibited matrine-induced autophagic flux in HepG2 and Bel7402 cells.

Effects of beclin-1 knockdown on matrine-induced autophagic flux and apoptosis in HepG2 and Bel7402 cells.

7 images

(5 references)

Catalog No. ABIN682153

Datasheet as PDF

Target See all SQSTM1 Antibodies

SQSTM1 (Sequestosome 1 (SQSTM1))
Binding Specificity
19

17

14

13

11

11

8

7

7

7

7

6

6

5

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 51-150
Reactivity
171

63

60

17

4

4

4

4

3

2

2

2

1

1

1

1
Human, Mouse, Rat, Cow
Host
145

24

1

1
Rabbit
Compatible Secondaries
Clonality
144

27
Polyclonal
Conjugate
77

13

12

8

8

8

5

5

5

5

5

5

2

2

2

2

2

1

1

1

1

1
This SQSTM1 antibody is un-conjugated
Application
121

57

50

40

25

22

18

16

13

12

10

4

3

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Cow, Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human SQSTM1/Ubiquitin-binding protein p62

Isotype

IgG

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Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Kuo, Weng, Chang, Huang, Lin, Chuang, Lin, Liou, Tai, Lin, Wang: "Altered mitochondrial dynamics and response to insulin in cybrid cells harboring a diabetes-susceptible mitochondrial DNA haplogroup." in: Free radical biology & medicine, Vol. 96, pp. 116-29, (2016) (PubMed).

Xia, Gao, Zhang, Wang, Zhao, Che, Ao, Yang, Wang, Lei: "Autophagy mediated by arginine depletion activation of the nutrient sensor GCN2 contributes to interferon-γ-induced malignant transformation of primary bovine mammary epithelial cells." in: Cell death discovery, Vol. 2, pp. 15065, (2016) (PubMed).

Cui, Luo, Li, Li, Wang: "Changes of intracellular Ca2+ in quercetin-induced autophagy progression." in: Acta biochimica et biophysica Sinica, Vol. 47, Issue 11, pp. 908-14, (2015) (PubMed).

Chen, Wei, Nie, Lin, Tian, Liu, Yu, Cheng, Yan, Wang, Liu, Deng, Lai, Zhou, Zhang, Lin, Chen: "?-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." in: Brain research, Vol. 1552, pp. 41-54, (2014) (PubMed).

Wang, Gao, Yao, Xie: "Blocking autophagic flux enhances matrine-induced apoptosis in human hepatoma cells." in: International journal of molecular sciences, Vol. 14, Issue 12, pp. 23212-30, (2014) (PubMed).
Target

SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name

SQSTM1 (SQSTM1 Products)

Synonyms

SQSTM1 antibody, sqstm1 antibody, MGC79491 antibody, A170 antibody, OSIL antibody, PDB3 antibody, ZIP3 antibody, p60 antibody, p62 antibody, p62B antibody, OSF-6 antibody, Osi antibody, STAP antibody, ZIP antibody, sb:cb621 antibody, zgc:85784 antibody, sequestosome 1 antibody, sequestosome 1 L homeolog antibody, SQSTM1 antibody, sqstm1 antibody, Sqstm1 antibody, sqstm1.L antibody

Background

Synonyms: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, SQSTM1, ORCA
Background: Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.

Gene ID

8878

UniProt

Q13501

Pathways

NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy