FLT3 Protein (AA 27-541) (Fc Tag)
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- Target See all FLT3 Proteins
- FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-541
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FLT3 protein is labelled with Fc Tag.
- Purpose
- Human FLT3/Flk-2 Protein
- Sequence
- Asn27-Asn541
- Characteristics
- Recombinant Human FLT3/Flk-2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asn27-Asn541.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FLT3 Ligand, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 19.6ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))
- Alternative Name
- FLT3 (FLT3 Products)
- Synonyms
- FLT3 Protein, B230315G04 Protein, CD135 Protein, Flk-2 Protein, Flk2 Protein, Flt-3 Protein, Ly72 Protein, wmfl Protein, FLK-2 Protein, FLK2 Protein, STK1 Protein, fms related tyrosine kinase 3 Protein, FMS-like tyrosine kinase 3 Protein, fms-related tyrosine kinase 3 Protein, FLT3 Protein, Flt3 Protein
- Background
- The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.
- Molecular Weight
- 84.9 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.
- Pathways
- RTK Signaling
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