IL12RB1 Protein (AA 24-540) (His tag)
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- Target See all IL12RB1 Proteins
- IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-540
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL12RB1 protein is labelled with His tag.
- Purpose
- Human IL-12 R beta 1/CD212 Protein
- Sequence
- Cys24-Glu540
- Characteristics
- Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys24-Glu540.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))
- Alternative Name
- IL-12 R beta 1 (IL12RB1 Products)
- Synonyms
- Il12rb Protein, IL12RB1 Protein, IL-12R Protein, CD212 Protein, IL-12R-BETA1 Protein, IL12RB Protein, IL-12R[b] Protein, interleukin 12 receptor subunit beta 1 Protein, interleukin 12 receptor, beta 1 Protein, Il12rb1 Protein, IL12RB1 Protein
- Background
- Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function).
- Molecular Weight
- 58.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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