ATP5J antibody (AA 1-108)
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- Target See all ATP5J Antibodies
- ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))
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Binding Specificity
- AA 1-108
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATP5J antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Polyclonal Antibody to Coupling Factor 6 (CF6)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against CF6. It has been selected for its ability to recognize CF6 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Coupling Factor 6 (CF6) corresdonding to Met1~Ala108 with N-terminal His Tag
- Isotype
- IgG
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- Application Notes
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Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))
- Alternative Name
- Coupling Factor 6 (ATP5J Products)
- Synonyms
- zgc:77541 antibody, wu:fa66e04 antibody, ATP5 antibody, ATP5A antibody, ATPM antibody, CF6 antibody, F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6 L homeolog antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F antibody, atp5j antibody, ATP5J antibody, atp5j.L antibody, Atp5j antibody
- Background
- ATP5J, ATP5A, ATP5, ATPM, ATP Synthase,H+ Transporting,Mitochondrial Fo Complex,Subunit F6, ATP synthase-coupling factor 6, mitochondrial
- Pathways
- Proton Transport, Ribonucleoside Biosynthetic Process
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