RUNX1 antibody (N-Term)
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- Target See all RUNX1 Antibodies
- RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Guinea Pig, Monkey, Chicken, Zebrafish (Danio rerio), Bat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RUNX1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human RUNX1
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Xenopus (92%) Zebrafish (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from N-Terminus of human RUNX1 (Q01196). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Chicken (100%), Opossum, Zebra finch, Xenopus (92%), Zebrafish (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product RUNX1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- After adding water, will consist of PBS buffer with 2 % sucrose
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))
- Alternative Name
- AML1 / RUNX1 (RUNX1 Products)
- Synonyms
- RUNX1 antibody, runx1 antibody, AI462102 antibody, AML1 antibody, Cbfa2 antibody, Pebp2a2 antibody, Pebpa2b antibody, AML1-EVI-1 antibody, AMLCR1 antibody, CBFA2 antibody, EVI-1 antibody, PEBP2aB antibody, runxa antibody, Runx-1 antibody, XAML antibody, Xaml1 antibody, aml antibody, aml-1 antibody, aml1 antibody, aml1-evi-1 antibody, amlcr1 antibody, cbfa2 antibody, evi-1 antibody, pebp2ab antibody, Aml1 antibody, uncharacterized LOC473981 antibody, runt-related transcription factor antibody, runt related transcription factor 1 antibody, runt-related transcription factor 1 antibody, runt related transcription factor 1 L homeolog antibody, LOC473981 antibody, runt antibody, Runx1 antibody, RUNX1 antibody, runx1 antibody, runx1.L antibody
- Background
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Name/Gene ID: RUNX1
Synonyms: RUNX1, AMLCR1, Aml1 oncogene, AML1, AML1-EVI-1, CBF-alpha-2, CBFA2, Acute myeloid leukemia 1, EVI-1, PEBP2A2, PEA2-alpha B, AML1-EVI-1 fusion protein, Oncogene AML-1, PEBP2-alpha B, PEBP2aB - Gene ID
- 861
- UniProt
- Q01196
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