VHL antibody
(1 reference)-
- Target See all VHL Antibodies
- VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This VHL antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Cross-Reactivity
- Human, Mouse
- Characteristics
-
Rabbit Polyclonal antibody to Von Hippel Lindau (von Hippel-Lindau tumor suppressor)
Von Hippel Lindau antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human Von Hippel Lindau. The exact sequence is proprietary.
- Isotype
- IgG
-
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 1-172) antibody
VHL Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 43-71), (N-Term) antibodyVHL Reactivity: Human, Mouse WB, FACS, IF, IHC (p) Host: Rabbit Polyclonal RB18667 unconjugated
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) antibodyVHL Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 1E1 unconjugated
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 6-175) antibodyVHL Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 181-210), (C-Term) antibodyVHL Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB18668 unconjugated
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 43-71) antibodyVHL Reactivity: Human, Mouse WB, IHC, ELISA, FACS, IF Host: Rabbit Polyclonal unconjugated
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) antibodyVHL Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 1-110) antibodyVHL Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1G12 unconjugated
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (AA 181-210) antibodyVHL Reactivity: Human WB, IHC, ELISA, FACS Host: Rabbit Polyclonal unconjugated
anti-Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL) (Internal Region) antibodyVerified VHL Reactivity: Human, Mouse, Rat WB, IHC, ELISA Host: Goat Polyclonal unconjugated
-
- Application Notes
- WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
-
Positive Control: Myc-DDK-tagged VHL-transfected 293T
Validation: Overexpression
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.27 mg/mL
- Buffer
- 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
-
: "An ID2-dependent mechanism for VHL inactivation in cancer." in: Nature, Vol. 529, Issue 7585, pp. 172-7, (2016) (PubMed).
-
: "An ID2-dependent mechanism for VHL inactivation in cancer." in: Nature, Vol. 529, Issue 7585, pp. 172-7, (2016) (PubMed).
-
- Target
- VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))
- Alternative Name
- von Hippel-Lindau tumor suppressor (VHL Products)
- Synonyms
- HRCA1 antibody, RCA1 antibody, VHL1 antibody, pVHL antibody, BcDNA:RH61560 antibody, CG13221 antibody, DVhl antibody, Dmel\\CG13221 antibody, Dvhl antibody, VHL antibody, d-VHL antibody, d-vhl antibody, dVHL antibody, dmVHL antibody, vhl antibody, rca1 antibody, vhl1 antibody, hrca1 antibody, zgc:158722 antibody, Vhlh antibody, von Hippel-Lindau tumor suppressor antibody, von Hippel-Lindau antibody, von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase antibody, von Hippel-Lindau disease tumor suppressor antibody, VHL antibody, Vhl antibody, vhl antibody, CpipJ_CPIJ009992 antibody
- Background
-
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Cellular Localization: Isoform 1: Cytoplasm , Membrane, Peripheral membrane protein , Nucleus , Isoform 3: Cytoplasm - Molecular Weight
- 24 kDa
- Gene ID
- 7428
- UniProt
- P40337
- Pathways
- Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Ubiquitin Proteasome Pathway
-
