WEE1 antibody (pSer642)
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- Target See all WEE1 Antibodies
- WEE1 (WEE1 Homolog (S. Pombe) (WEE1))
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Binding Specificity
- pSer642
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This WEE1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Predicted Reactivity
- Human,Mouse,Rat,Dog,Cow,Pig,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser642 [SV(p-S)LT]
- Isotype
- IgG
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anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer123) antibody
WEE1 Reactivity: Mouse WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer123) antibodyWEE1 Reactivity: Human IHC (p), DB Host: Rabbit Polyclonal RB07865 unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 289-388) antibodyWEE1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 5B6 unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 604-634), (C-Term) antibodyWEE1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB3525 unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 144-173) antibodyWEE1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB3524 unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) antibodyWEE1 Reactivity: Human, Mouse, Rat ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 1-646) antibodyWEE1 Reactivity: Human WB, PLA Host: Rabbit Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 1-646) antibodyWEE1 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer123) antibodyWEE1 Reactivity: Human ELISA, IHC, DB Host: Rabbit Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 604-634) antibodyWEE1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- WEE1 (WEE1 Homolog (S. Pombe) (WEE1))
- Alternative Name
- Wee1 (WEE1 Products)
- Synonyms
- WEE1A antibody, WEE1hu antibody, ATWEE1 antibody, F22D16.3 antibody, F22D16_3 antibody, WEE1 kinase homolog antibody, WEE1-KINASE antibody, WEE1 antibody, Xe-Wee1B antibody, XeWee1B antibody, wee1-b antibody, wee1a antibody, wee2-a antibody, xe-wee1a antibody, xwee1 antibody, wee1 antibody, wee1b antibody, wee1hu antibody, xwee2 antibody, GB19689 antibody, Wee1A antibody, fa20h03 antibody, wu:fa20h03 antibody, zgc:136514 antibody, WEE1 G2 checkpoint kinase antibody, Wee1 kinase antibody, WEE1-like kinase antibody, WEE1 G2 checkpoint kinase S homeolog antibody, wee1 homolog antibody, WEE1 homolog 2 antibody, wee1-like protein kinase antibody, WEE 1 homolog 1 (S. pombe) antibody, M phase inhibitor protein kinase Wee1 antibody, WEE1 antibody, Wee1 antibody, wee1.S antibody, wee1-a antibody, wee2 antibody, LOC551677 antibody, wee1 antibody
- Background
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Synonyms: WEE1A, WEE1hu, Wee1-like protein kinase, Wee1A kinase, WEE1
Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
- Gene ID
- 7465
- UniProt
- P30291
- Pathways
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
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