TNFSF8 Protein (AA 63-234) (mFc Tag)
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- Target See all TNFSF8 Proteins
- TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 63-234
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFSF8 protein is labelled with mFc Tag.
- Purpose
- Human CD30 Ligand/TNFSF8 Protein
- Sequence
- Gln63-Asp234
- Characteristics
- Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Gln63-Asp234.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC,
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD30, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Human CD30 Ligand, mFc (IgG2a) Tag with the EC50 of 31.9ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
- Alternative Name
- CD30 Ligand (TNFSF8 Products)
- Synonyms
- CD153 Protein, CD30LG Protein, Cd30l Protein, CD30L Protein, TNFRSF8 Protein, TNFSF8 Protein, tumor necrosis factor (ligand) superfamily, member 8 Protein, TNF superfamily member 8 Protein, tumor necrosis factor superfamily member 8 Protein, Tnfsf8 Protein, TNFSF8 Protein
- Background
- CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
- Molecular Weight
- 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
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