Mouse IgG isotype control (His tag)

Details for Product No. ABIN1098612, Supplier: Log in to see
Isotype
Host
Mouse
217
206
204
169
160
127
112
93
88
88
88
73
56
50
43
42
27
25
24
22
21
11
8
3
3
2
1
Antibody Type
Recombinant
Conjugate
His tag
206
169
167
166
154
108
58
58
48
44
19
13
12
12
11
9
9
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Isotype Control (IsoC), SDS-PAGE (SDS)
Options
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Isotype IgG
Purification purified by using conventional chromatography.
Purity > 90 % by SDS-PAGE
Background Immunoglobulin G (IgG) is antibody molecules. Each IgG is composed of four peptide chains - two heavy chains gamma and two light chains. Each IgG has two antigen binding sites. IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response. It also plays an important role in Antibody-dependent cell-mediated cytotoxicity(ADCC) and Intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol. Recombinant mouse IgG protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.
Synonyms: Immunoglobulin heavy chain constant region gamma 2a, Immunoglobulin G
Molecular Weight 27.5 kDa (242aa)
Research Area Immunology, Secondary Antibodies
Application Notes Each Investigator should determine their own optimal working dilution for specific applications.
Comment

Concentration (detection method): 1 mg/mL (determined by Bradford assay)

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 20 mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C
Supplier Images
 image for Mouse IgG isotype control (His tag) (ABIN1098612) Mouse IgG isotype control (His tag)
Background publications Mallery, McEwan, Bidgood, Towers, Johnson, James: "Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 46, pp. 19985-90, 2010 (PubMed).

Stadlmann, Pabst, Kolarich, Kunert, Altmann: "Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides." in: Proteomics, Vol. 8, Issue 14, pp. 2858-71, 2008 (PubMed).