Human IgG Isotype Control

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Isotype
Host
Human
217
207
204
168
160
127
112
93
88
88
88
73
56
50
43
42
27
25
24
22
21
11
8
3
3
2
1
Application
Dot Blot (DB), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Isotype Control (IsoC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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Isotype IgG
Fragment Whole molecule
Characteristics Purified Human IgG (Whole Molecule) Control is purified from normal human serum through Protein G chromatography. Purified Human IgG (Whole Molecule) Control can be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Western and Dot blots), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reactions.
Purification Protein G chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Each Investigator should determine their own optimal working dilution for specific applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 4 mg/mL.
Concentration 4 mg/mL
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Product cited in: van Zeijl, van Koppen, Surovtseva, de Gooyer, Plate, Conti, Karstens, Timmers, Saeed, Wiersinga, Miltenburg, Fliers, Boelen: "Complete inhibition of rhTSH-, Graves' disease IgG-, and M22-induced cAMP production in differentiated orbital fibroblasts by a low-molecular-weight TSHR antagonist." in: The Journal of clinical endocrinology and metabolism, Vol. 97, Issue 5, pp. E781-5, 2012 (PubMed).

Calvert, Kalantarov, Chang, Trakht, Blair, Roehrig: "Human monoclonal antibodies to West Nile virus identify epitopes on the prM protein." in: Virology, Vol. 410, Issue 1, pp. 30-7, 2011 (PubMed).