HEK-293 Cells IgG isotype control (His tag)

Details for Product No. ABIN2870651, Supplier: Log in to see
Isotype
Reactivity
Human
Host
HEK-293 Cells
215
208
201
170
161
131
114
94
94
93
92
87
78
76
44
38
25
23
22
8
6
4
2
Antibody Type
Recombinant Antibody
Biological Activity
Active
Conjugate
His tag
207
168
165
164
155
108
58
58
48
41
24
13
12
12
11
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Isotype Control (IsoC)
Options
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Isotype IgG
Fragment Fc fragment
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
Molecular Weight 27 kDa
UniProt P01857
Research Area Secondary Antibodies
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for HEK-293 Cells IgG isotype control (His tag) (ABIN2870651) Human IgG1 Fc, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ...