Mouse IgG Isotype Control

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Isotype
Host
Mouse
217
206
204
169
160
127
112
93
88
88
88
73
56
50
43
42
27
25
24
22
21
11
8
3
3
2
1
Application
Blocking Reagent (BR), Dot Blot (DB), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Immunoprecipitation (IP), Isotype Control (IsoC), ELISA, Western Blotting (WB)
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Isotype IgG
Fragment Whole molecule
Characteristics Purified Mouse IgG (Whole Molecule) Control is highly purified from normal mouse serum through Protein A chromatography.Purified Mouse IgG (Whole Molecule) Control is suitable to be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reaction.
Purification Protein A chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Each Investigator should determine their own optimal working dilution for specific applications.
Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing cycles.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Product cited in: Iorio, Festa, Rosati, Hahne, Tiberti, Capunzo, De Laurenzi, Turco: "BAG3 regulates formation of the SNARE complex and insulin secretion." in: Cell death & disease, Vol. 6, pp. e1684, 2015 (PubMed).

López-Falcón, Meyer-Nava, Hernández-Rodríguez, Campos, Montero, Rudiño, Vázquez, Zurita, Valadez-Graham: "Characterization of the Drosophila group ortholog to the amino-terminus of the alpha-thalassemia and mental retardation X-Linked (ATRX) vertebrate protein." in: PLoS ONE, Vol. 9, Issue 12, pp. e113182, 2014 (PubMed).