Human IgG isotype control (PE)
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- Target See all IgG products
- IgG
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Host
- Human
- Conjugate
- PE
- Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)
- Cross-Reactivity (Details)
- Human IgG whole molecule Phycoerythrin conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Human IgG and anti-Human Serum.
- Purification
- Human IgG whole molecule Phycoerythrin conjugated was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Isotype
- IgG
- Labeling Ratio
- 1-2
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Rabbit IgG isotype control
IsoC, ELISA, WB IgG Native unconjugated
Mouse IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlELISA, WB, IHC IgG unconjugated
Rabbit IgG isotype control (PE)ELISA, FACS Polyclonal IgG PE
Goat IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Rabbit IgG isotype controlELISA, FACS Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
Rat IgG isotype controlELISA, WB IgG unconjugated
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- Application Notes
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Flow Cytometry Dilution: 1:500-1:2500
Immunohistochemistry Dilution: 1:500-1:2500
Application Note: Human IgG whole molecule Phycoerythrin conjugated can be utilized as a control reagent in Flow Cytometry, Immunohistochemistry, and Western Blotting. Researchers should determine optimal titers for applications that are not stated.
Western Blot Dilution: 1:1,000-1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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