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anti-Human CD25 antibody for Intracellular Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Human
442
332
109
19
10
10
8
5
4
3
3
2
1
Host
Mouse
481
303
89
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to FITC
113
113
91
61
28
17
15
13
12
10
10
7
6
6
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
675
130
117
95
92
70
59
47
45
20
17
15
14
11
10
9
6
6
5
3
2
2
2
1
1
1
Supplier
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Available images

Catalog No. ABIN2661381
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008704084 ABIN2664435 ICFC Neut Mouse IgG1 kappa Log in to see M-A251 3
0.0008704084 ABIN2661382 FACS ICFC FITC Mouse IgG1 kappa Log in to see M-A251 3
0.0008704084 ABIN2664436 ICFC Neut Mouse IgG1 kappa Log in to see M-A251 3

Top referenced anti-CD25 antibody for Intracellular Flow Cytometry

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Application / Reactivity Human
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ELISA 59 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 301 Antibodies
Functional Studies (Func) 13 Antibodies
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 26 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(442), (332), (109), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1)
Host Mouse
(481), (303), (89), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to FITC
(113), (113), (91), (61), (28), (17), (15), (13), (12), (10), (10), (7), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
(675), (130), (117), (95), (92), (70), (59), (47), (45), (20), (17), (15), (14), (11), (10), (9), (6), (6), (5), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2661381) Images
Cross-Reactivity Baboon, Cynomolgus, Rhesus Monkey
Purification The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Clone M-A251
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2661381) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2661381) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2661381) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD25 Antibody (ABIN2661381)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Li, Pauza: "CD25(+) Bcl6(low) T follicular helper cells provide help to maturing B cells in germinal centers of human tonsil." in: European journal of immunology, Vol. 45, Issue 1, pp. 298-308, 2015

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2661381) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (FITC) (ABIN2661381) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (FITC)