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anti-Human CD25 antibody for Immunofluorescence

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Epitope
pSer268
22
20
14
13
12
11
7
7
7
6
5
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
568
457
169
19
10
10
8
5
4
3
3
2
1
1
Host
Rabbit
602
406
133
5
2
Clonality
Polyclonal
Conjugate
This CD25 antibody is un-conjugated
130
124
101
61
30
28
25
25
22
19
17
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
937
145
140
128
127
121
80
71
48
21
18
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN1531665
€ 288.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3215331 ABIN1532689 IF ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.3215331 ABIN2490712 IF ELISA WB Rabbit IgG Ser268 Log in to see Polyclonal
0.3215331 ABIN2490713 IF ELISA WB Rabbit IgG pSer268 Log in to see Polyclonal
0.001079923 ABIN1106143 IF PE Mouse IgG1 Log in to see CD25-3G10 2
0.001079923 ABIN1106142 IF FITC Mouse IgG1 Log in to see CD25-3G10 5
0.001079923 ABIN2661003 FACS IF IHC Biotin Mouse IgG1 kappa Log in to see BC96 5
0.001079923 ABIN284166 FACS IF Mouse IgG2b Log in to see CLB-IL2R-TB30
0.001079923 ABIN2659808 FACS IF WB PerCP Mouse IgG1 kappa Log in to see M-A251 3
0.001079923 ABIN2663966 IF WB PE Mouse IgG1 kappa Log in to see BC96 7
0.001079923 ABIN2664013 FACS IF IHC Alexa Fluor 488 Mouse IgG1 kappa Log in to see BC96 4
0.001079923 ABIN2888884 IF ELISA WB Rabbit IgG AA 223-272 Log in to see Polyclonal
0.001079923 ABIN498207 IF WB Rabbit Log in to see Polyclonal
0.001079923 ABIN153451 ICC IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see IL2R-1
0.001079923 ABIN1106139 IHC (fro) IF Mouse IgG1 Log in to see RO-G10
0.001079923 ABIN1106140 IHC (fro) IF Mouse IgG3 Log in to see BL-D-D1
0.001079923 ABIN2659809 FACS IF WB PerCP Mouse IgG1 kappa Log in to see M-A251 4
0.001079923 ABIN2663967 IF WB PE Mouse IgG1 kappa Log in to see BC96 7
0.001079923 ABIN2664014 FACS IF IHC Alexa Fluor 488 Mouse IgG1 kappa Log in to see BC96 4
0.001079923 ABIN2851747 FACS IF Biotin Mouse IgG2a Log in to see
0.001079923 ABIN2851792 FACS IF FITC Mouse IgG2a Log in to see

Top referenced anti-CD25 antibody for Immunofluorescence

  • Human Monoclonal CD25 Primary Antibody for IF - ABIN1106143 (2 Publications): Leonard, Depper, Uchiyama, Smith, Waldmann, Greene: A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor; partial characterization of the receptor. in Nature 1983 (PubMed)

  • Human Monoclonal CD25 Primary Antibody for IF - ABIN1106142 (5 Publications): Uchiyama, Nelson, Fleisher, Waldmann: A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. II. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells. in Journal of immunology (Baltimore, Md. : 1950) 1981 (PubMed)

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 61 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 420 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 68 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope pSer268
(22), (20), (14), (13), (12), (11), (7), (7), (7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(568), (457), (169), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Rabbit
(602), (406), (133), (5), (2)
Clonality
Polyclonal
Conjugate This CD25 antibody is un-conjugated
(130), (124), (101), (61), (30), (28), (25), (25), (22), (19), (17), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(937), (145), (140), (128), (127), (121), (80), (71), (48), (21), (18), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1531665) Images
Specificity IL-2R alpha/CD25 (Phospho-Ser268) Antibody detects endogenous levels of IL-2R alpha/CD25 only when phosphorylated at Ser268.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IL-2R alpha/CD25 around the phosphorylation site of Ser268.
Isotype IgG

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1531665) Images back to top
Antigen
Alternative Name IL-2R alpha/cd25 (IL2RA Antibody Abstract)
Background Molecular weight of target protein: 30 kDa
Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen, CD25
Molecular Weight 30 kDa
Gene ID 3559
OMIM 147730
UniProt P01589
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1531665) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:10000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.231367 (NCBI Gene Symbol: IL2RA)

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1531665) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-CD25 Antibody (ABIN1531665)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Bamborough, Hedgecock, Richards: "The interleukin-2 and interleukin-4 receptors studied by molecular modelling." in: Structure (London, England : 1993), Vol. 2, Issue 9, pp. 839-51, 1995

Nikaido, Shimizu, Ishida, Sabe, Teshigawara, Maeda, Uchiyama, Yodoi, Honjo: "Molecular cloning of cDNA encoding human interleukin-2 receptor." in: Nature, Vol. 311, Issue 5987, pp. 631-5, 1984

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1531665) back to top
Supplier Images
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (pSer268) (ABIN1531665) Western blot analysis of extracts from LOVO cells, using IL-2R alpha/CD25 (Phospho-Se...
Immunofluorescence (IF) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (pSer268) (ABIN1531665) Immunofluorescence analysis of HeLa cells, using IL-2R alpha/CD25 (Phospho-Ser268) An...