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anti-Human CD25 antibody for Immunofluorescence

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Epitope
pSer268
22
20
19
18
15
15
14
13
13
7
6
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
556
438
152
17
14
9
8
4
4
3
3
2
1
1
Host
Rabbit
554
379
140
7
5
Clonality
Polyclonal
Conjugate
This CD25 antibody is un-conjugated
126
112
90
54
29
27
26
23
22
22
17
16
12
11
11
11
11
11
10
8
8
8
8
8
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
882
154
148
142
107
83
78
68
45
21
19
17
16
14
13
12
9
7
6
6
5
4
3
2
2
1
1
1
1
Options
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Catalog No. ABIN1531665
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.813965 ABIN1532689 IF ELISA WB Rabbit IgG Log in to see Polyclonal 2
11.813965 ABIN4291000 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 1B5D12
1 ABIN1106142 IF FITC Mouse IgG1 Log in to see CD25-3G10 5
1 ABIN1106143 IF PE Mouse IgG1 Log in to see CD25-3G10 2
1 ABIN153451 ICC IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see IL2R-1 5
1 ABIN2661003 FACS IF IHC Biotin Mouse IgG1 kappa Log in to see BC96 5
1 ABIN2663966 IF WB PE Mouse IgG1 kappa Log in to see BC96 7
1 ABIN2664013 FACS IF IHC Alexa Fluor 488 Mouse IgG1 kappa Log in to see BC96 4
1 ABIN2659808 FACS IF WB PerCP Mouse IgG1 kappa Log in to see M-A251 3
1 ABIN2888884 IF ELISA WB Rabbit IgG AA 223-272 Log in to see Polyclonal
1 ABIN498207 IF WB Rabbit Log in to see Polyclonal
1 ABIN1106139 IHC (fro) IF Mouse IgG1 Log in to see RO-G10
1 ABIN1106140 IHC (fro) IF Mouse IgG3 Log in to see BL-D-D1
1 ABIN2851792 FACS IF FITC Mouse IgG2a Log in to see
1 ABIN2663967 IF WB PE Mouse IgG1 kappa Log in to see BC96 7
1 ABIN2664014 FACS IF IHC Alexa Fluor 488 Mouse IgG1 kappa Log in to see BC96 4
1 ABIN2851681 FACS IF PE Mouse IgG2a Log in to see
1 ABIN2851731 FACS IF PE-Cy5 Mouse IgG2a Log in to see
1 ABIN2851747 FACS IF Biotin Mouse IgG2a Log in to see
1 ABIN2659809 FACS IF WB PerCP Mouse IgG1 kappa Log in to see M-A251 4

Top referenced anti-CD25 antibody for Immunofluorescence

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Cytometry by Time of Flight (CyTOF) 8 Antibodies
ELISA 60 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 407 Antibodies
Functional Studies (Func) 13 Antibodies
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Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
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Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope pSer268
(22), (20), (19), (18), (15), (15), (14), (13), (13), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(556), (438), (152), (17), (14), (9), (8), (4), (4), (3), (3), (2), (1), (1)
Host Rabbit
(554), (379), (140), (7), (5)
Clonality
Polyclonal
Conjugate This CD25 antibody is un-conjugated
(126), (112), (90), (54), (29), (27), (26), (23), (22), (22), (17), (16), (12), (11), (11), (11), (11), (11), (10), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(882), (154), (148), (142), (107), (83), (78), (68), (45), (21), (19), (17), (16), (14), (13), (12), (9), (7), (6), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1531665) Images
Specificity IL-2R alpha/CD25 (Phospho-Ser268) Antibody detects endogenous levels of IL-2R alpha/CD25 only when phosphorylated at Ser268.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IL-2R alpha/CD25 around the phosphorylation site of Ser268.
Isotype IgG

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN1531665) Images back to top
Antigen
Alternative Name IL-2R alpha/cd25 (IL2RA Antibody Abstract)
Background Molecular weight of target protein: 30 kDa
Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen, CD25
Molecular Weight 30 kDa
Gene ID 3559
OMIM 147730
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN1531665) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:10000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.231367 (NCBI Gene Symbol: IL2RA)

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN1531665) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-CD25 Antibody (ABIN1531665)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Bamborough, Hedgecock, Richards: "The interleukin-2 and interleukin-4 receptors studied by molecular modelling." in: Structure (London, England : 1993), Vol. 2, Issue 9, pp. 839-51, 1995

Nikaido, Shimizu, Ishida, Sabe, Teshigawara, Maeda, Uchiyama, Yodoi, Honjo: "Molecular cloning of cDNA encoding human interleukin-2 receptor." in: Nature, Vol. 311, Issue 5987, pp. 631-5, 1984

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN1531665) back to top
Supplier Images
Western Blotting (WB) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (pSer268) (ABIN1531665) Western blot analysis of extracts from LOVO cells, using IL-2R alpha/CD25 (Phospho-Se...
Immunofluorescence (IF) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha) (pSer268) (ABIN1531665) Immunofluorescence analysis of HeLa cells, using IL-2R alpha/CD25 (Phospho-Ser268) An...