anti-Human CD25 antibody for Immunohistochemistry (Acetone-fixed)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Human
603
498
175
22
19
15
10
8
6
6
4
4
3
3
2
1
Host
Mouse
609
424
152
8
6
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
137
119
96
62
36
31
31
26
23
22
21
16
14
12
12
12
12
12
12
11
10
10
10
10
9
8
6
6
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
979
187
171
156
126
90
85
82
61
29
26
21
20
17
13
12
9
7
6
6
6
5
3
2
1
1
1
Options
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Catalog No. ABIN965780
$ 206.25
Plus shipping costs $45.00

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(603), (498), (175), (22), (19), (15), (10), (8), (6), (6), (4), (4), (3), (3), (2), (1)
Host Mouse
(609), (424), (152), (8), (6), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(137), (119), (96), (62), (36), (31), (31), (26), (23), (22), (21), (16), (14), (12), (12), (12), (12), (12), (12), (11), (10), (10), (10), (10), (9), (8), (6), (6), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(979), (187), (171), (156), (126), (90), (85), (82), (61), (29), (26), (21), (20), (17), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN965780) Images
Specificity This antibody reacts a 55kD single chain glycoprotein, CD25, that associates with CD12 and CD132 to form the IL-2 receptor complex. CD25 has three epitope regions - A, B, and C - the latter of which is recognized by B489. This MAb has been clustered at the Fourth International Workshop on human differentiation antigens.
Purification Concentrated.
Immunogen Human leucocytes.
Clone B489 (143-13)
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN965780) Images back to top
Antigen
Alternative Name CD25 (IL-2R) (IL2RA Antibody Abstract)
Gene ID 3559
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN965780) Images back to top
Application Notes Immunohistochemistry (acetone fixed frozen tissue): 1:50-1:100
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen tissue sections (acetone fixed). The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN965780) Images back to top
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-CD25 antibody (ABIN965780)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Parke: "Intravenous gammaglobulin in pregnancy, the Connecticut experience." in: Scandinavian journal of rheumatology. Supplement, Vol. 107, pp. 103-4, 1998

Goldstein, Marcon, Cullen, Nelson: "A competitive enzyme-linked immunoassay (ELISA) for the measurement of soluble human interleukin-2 receptors (IL-2R, Tac protein)." in: Journal of immunological methods, Vol. 107, Issue 1, pp. 103-9, 1988

Uchiyama, Nelson, Fleisher, Waldmann: "A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. II. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 4, pp. 1398-403, 1981