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anti-Human CD25 antibody for Immunohistochemistry (Acetone-fixed)

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Human
568
457
169
19
10
10
8
5
4
3
3
2
1
1
Host
Mouse
601
406
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
130
124
101
61
30
28
25
25
22
19
17
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
936
145
141
129
127
120
80
72
48
21
18
18
17
11
9
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN965780
$ 206.25
Plus shipping costs $45.00

Top referenced anti-CD25 antibody for Immunohistochemistry (Acetone-fixed)

  • Human Monoclonal CD25 Primary Antibody for IHC (af), IHC (fro) - ABIN965780 (3 Publications): Uchiyama, Nelson, Fleisher, Waldmann: A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. II. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells. in Journal of immunology (Baltimore, Md. : 1950) 1981 (PubMed)

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 61 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 420 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 68 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 64 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 27 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(568), (457), (169), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(601), (406), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (124), (101), (61), (30), (28), (25), (25), (22), (19), (17), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(936), (145), (141), (129), (127), (120), (80), (72), (48), (21), (18), (18), (17), (11), (9), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN965780) Images
Specificity This antibody reacts a 55kD single chain glycoprotein, CD25, that associates with CD12 and CD132 to form the IL-2 receptor complex. CD25 has three epitope regions - A, B, and C - the latter of which is recognized by B489. This MAb has been clustered at the Fourth International Workshop on human differentiation antigens.
Purification Concentrated.
Immunogen Human leucocytes.
Clone B489 (143-13)
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN965780) Images back to top
Antigen
Alternative Name CD25 (IL-2R) (IL2RA Antibody Abstract)
Gene ID 3559
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN965780) Images back to top
Application Notes Immunohistochemistry (acetone fixed frozen tissue): 1:50-1:100
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen tissue sections (acetone fixed). The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN965780) Images back to top
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-CD25 Antibody (ABIN965780)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Parke: "Intravenous gammaglobulin in pregnancy, the Connecticut experience." in: Scandinavian journal of rheumatology. Supplement, Vol. 107, pp. 103-4, 1998

Goldstein, Marcon, Cullen, Nelson: "A competitive enzyme-linked immunoassay (ELISA) for the measurement of soluble human interleukin-2 receptors (IL-2R, Tac protein)." in: Journal of immunological methods, Vol. 107, Issue 1, pp. 103-9, 1988

Uchiyama, Nelson, Fleisher, Waldmann: "A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. II. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 4, pp. 1398-403, 1981