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anti-Human CD25 antibody for Immunoprecipitation

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Human
574
462
170
19
10
10
8
6
4
3
3
2
1
1
Host
Mouse
607
412
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to PerCP-Cy5.5
131
125
102
61
30
28
25
25
22
19
18
15
14
12
12
12
12
12
12
11
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
949
149
142
132
131
125
81
75
48
21
21
18
17
11
10
9
6
6
5
4
3
3
2
2
2
1
1
1
Supplier
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Available images

Catalog No. ABIN2660057
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000887791 ABIN2660324 IP IHC ELISA WB PerCP-Cy5.5 Mouse IgG1 kappa Log in to see BC96 4
0.000887791 ABIN2660659 IP WB APC-Cy7 Mouse IgG1 kappa Log in to see BC96 5
0.000887791 ABIN120760 FACS IP Mouse IgM Log in to see MEM-140
0.000887791 ABIN187250 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see IL2R-1
0.000887791 ABIN94049 FACS IP Mouse IgM Log in to see MEM-140 3
0.000887791 ABIN2660325 IP IHC ELISA WB PerCP-Cy5.5 Mouse IgG1 kappa Log in to see BC96 4
0.000887791 ABIN2660058 FACS IP IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see M-A251 3
0.000887791 ABIN2660660 IP WB APC-Cy7 Mouse IgG1 kappa Log in to see BC96 5
0.000887791 ABIN303574 FACS IP Biotin Mouse IgM Log in to see MEM-140
0.000887791 ABIN207006 FACS IP Mouse IgM Log in to see MEM-140
0.000887791 ABIN259702 FACS IP Biotin Mouse IgM Log in to see MEM-140
0.000887791 ABIN269086 FACS IP Mouse IgM Log in to see MEM-140
0.000887791 ABIN3199653 FACS IP Biotin Mouse IgM Log in to see MEM-140
0.000887791 ABIN2224471 FACS IP WB Mouse IgG1 Log in to see 3H1147
0.000887791 ABIN2224490 FACS IP FITC Mouse IgM Log in to see 14D28
0.000887791 ABIN2224489 FACS IP Biotin Mouse IgM Log in to see 14D28
0.000887791 ABIN2224492 FACS IP PE Mouse IgM Log in to see 14D28
0.000887791 ABIN2224515 FACS IP Alkaline Phosphatase (AP) Mouse IgM Log in to see 14D28
0.000887791 ABIN2224474 FACS IP Mouse IgM Log in to see 14D28
0.000887791 ABIN2224488 FACS IP APC Mouse IgM Log in to see 14D28

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 62 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 417 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 65 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies
Immunoprecipitation (IP) 27 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(574), (462), (170), (19), (10), (10), (8), (6), (4), (3), (3), (2), (1), (1)
Host Mouse
(607), (412), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to PerCP-Cy5.5
(131), (125), (102), (61), (30), (28), (25), (25), (22), (19), (18), (15), (14), (12), (12), (12), (12), (12), (12), (11), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(949), (149), (142), (132), (131), (125), (81), (75), (48), (21), (21), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2660057) Images
Cross-Reactivity Baboon, Cynomolgus, Rhesus Monkey
Purification The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Clone M-A251
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2660057) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2660057) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2660057) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD25 Antibody (ABIN2660057)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Li, Pauza: "CD25(+) Bcl6(low) T follicular helper cells provide help to maturing B cells in germinal centers of human tonsil." in: European journal of immunology, Vol. 45, Issue 1, pp. 298-308, 2015

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2660057) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (PerCP-Cy5.5) (ABIN2660057) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP-Cy5.5)