anti-Human CD25 antibody for Immunoprecipitation

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Human
591
480
166
22
19
15
9
8
6
6
4
4
3
3
2
1
Host
Mouse
589
406
152
7
5
3
2
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to PerCP-Cy5.5
132
119
96
79
30
29
29
24
23
18
16
14
12
12
12
12
12
10
10
10
9
8
8
7
7
6
6
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
940
186
168
155
125
90
85
83
61
29
21
21
19
17
13
12
9
7
6
6
6
5
3
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2660324
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2660057 FACS IP IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see M-A251 3
1 ABIN2660659 IP WB APC-Cy7 Mouse IgG1 kappa Log in to see BC96 5
1 ABIN4291008 FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 kappa Log in to see 2R12
1 ABIN120760 FACS IP Mouse IgM Log in to see MEM-140
1 ABIN187250 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see IL2R-1
1 ABIN94049 FACS IP Mouse IgM Log in to see MEM-140 3
1 ABIN94050 FACS IP Biotin Mouse IgM Log in to see MEM-140 3
1 ABIN259702 FACS IP Biotin Mouse IgM Log in to see MEM-140
1 ABIN269086 CyTOF FACS IP Mouse IgM Log in to see MEM-140
1 ABIN2660058 FACS IP IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see M-A251 3
1 ABIN2660660 IP WB APC-Cy7 Mouse IgG1 kappa Log in to see BC96 5
1 ABIN2660325 IP IHC ELISA WB PerCP-Cy5.5 Mouse IgG1 kappa Log in to see BC96 4
1 ABIN303574 FACS IP Biotin Mouse IgM Log in to see MEM-140
1 ABIN1859412 ICC IHC IP WB Rabbit IgG AA 22-213 Log in to see Polyclonal
1 ABIN207006 FACS IP Mouse IgM Log in to see MEM-140
1 ABIN3199652 FACS IP Mouse IgM Log in to see MEM-140
1 ABIN2224471 FACS IP WB Mouse IgG1 Log in to see 3H1147
1 ABIN2224474 FACS IP Mouse IgM Log in to see 14D28
1 ABIN3199653 FACS IP Biotin Mouse IgM Log in to see MEM-140
1 ABIN2224488 FACS IP APC Mouse IgM Log in to see 14D28

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(591), (480), (166), (22), (19), (15), (9), (8), (6), (6), (4), (4), (3), (3), (2), (1)
Host Mouse
(589), (406), (152), (7), (5), (3), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to PerCP-Cy5.5
(132), (119), (96), (79), (30), (29), (29), (24), (23), (18), (16), (14), (12), (12), (12), (12), (12), (10), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(940), (186), (168), (155), (125), (90), (85), (83), (61), (29), (21), (21), (19), (17), (13), (12), (9), (7), (6), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN2660324) Images
Cross-Reactivity Baboon, Chimpanzee, Cynomolgus, Rhesus Monkey, Pigtailed Macaque
Purification The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Clone BC96
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN2660324) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN2660324) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN2660324) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD25 antibody (ABIN2660324)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009

Ernst, Lee, Nakanishi, Karimbux, Rezende, Stashenko, Seki, Taubman, Kawai: "Diminished forkhead box P3/CD25 double-positive T regulatory cells are associated with the increased nuclear factor-kappaB ligand (RANKL+) T cells in bone resorption lesion of periodontal disease." in: Clinical and experimental immunology, Vol. 148, Issue 2, pp. 271-80, 2007

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN2660324) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP-Cy5.5) (ABIN2660324) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP-Cy5.5)