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anti-Human CD25 antibody for Immunoprecipitation

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Human
558
439
152
17
14
9
8
4
4
3
3
2
1
1
Host
Mouse
555
380
141
7
5
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to PerCP-Cy5.5
126
113
90
56
29
27
26
23
22
22
17
16
12
11
11
11
11
11
9
8
8
8
8
8
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
885
153
148
141
107
83
78
69
45
21
19
17
16
14
13
12
9
7
6
6
5
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2660324
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2660057 FACS IP IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see M-A251 3
1 ABIN2660659 IP WB APC-Cy7 Mouse IgG1 kappa Log in to see BC96 5
1 ABIN120760 FACS IP Mouse IgM Log in to see MEM-140
1 ABIN187250 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see IL2R-1
1 ABIN94050 FACS IP Biotin Mouse IgM Log in to see MEM-140 3
1 ABIN94049 FACS IP Mouse IgM Log in to see MEM-140 3
1 ABIN2660325 IP IHC ELISA WB PerCP-Cy5.5 Mouse IgG1 kappa Log in to see BC96 4
1 ABIN2660660 IP WB APC-Cy7 Mouse IgG1 kappa Log in to see BC96 5
1 ABIN2660058 FACS IP IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see M-A251 3
1 ABIN4291008 FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 kappa Log in to see 2R12
1 ABIN1859412 ICC IHC IP WB Rabbit IgG AA 22-213 Log in to see Polyclonal
1 ABIN303574 FACS IP Biotin Mouse IgM Log in to see MEM-140
1 ABIN207006 FACS IP Mouse IgM Log in to see MEM-140
1 ABIN259702 FACS IP Biotin Mouse IgM Log in to see MEM-140
1 ABIN269086 CyTOF FACS IP Mouse IgM Log in to see MEM-140
1 ABIN3199652 FACS IP Mouse IgM Log in to see MEM-140
1 ABIN3199653 FACS IP Biotin Mouse IgM Log in to see MEM-140
1 ABIN2224474 FACS IP Mouse IgM Log in to see 14D28
1 ABIN2224491 IP HRP Mouse IgM Log in to see 14D28
1 ABIN2224490 FACS IP FITC Mouse IgM Log in to see 14D28

Top referenced anti-CD25 antibody for Immunoprecipitation

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 4 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
ELISA 60 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 409 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 63 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(558), (439), (152), (17), (14), (9), (8), (4), (4), (3), (3), (2), (1), (1)
Host Mouse
(555), (380), (141), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to PerCP-Cy5.5
(126), (113), (90), (56), (29), (27), (26), (23), (22), (22), (17), (16), (12), (11), (11), (11), (11), (11), (9), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(885), (153), (148), (141), (107), (83), (78), (69), (45), (21), (19), (17), (16), (14), (13), (12), (9), (7), (6), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2660324) Images
Cross-Reactivity Baboon, Chimpanzee, Cynomolgus, Rhesus Monkey, Pigtailed Macaque
Purification The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Clone BC96
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2660324) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2660324) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2660324) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD25 Antibody (ABIN2660324)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009

Ernst, Lee, Nakanishi, Karimbux, Rezende, Stashenko, Seki, Taubman, Kawai: "Diminished forkhead box P3/CD25 double-positive T regulatory cells are associated with the increased nuclear factor-kappaB ligand (RANKL+) T cells in bone resorption lesion of periodontal disease." in: Clinical and experimental immunology, Vol. 148, Issue 2, pp. 271-80, 2007

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2660324) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (PerCP-Cy5.5) (ABIN2660324) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP-Cy5.5)