You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human CD25 antibody for Immunoprecipitation

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor, alpha
  • interferon stimulated exonuclease gene 20kDa
  • interleukin 2 receptor, alpha chain
  • LOC100222957
  • ISG20
  • IL2RA
  • Il2ra
Reactivity
Human
561
444
152
17
14
9
8
5
4
3
3
2
1
1
Host
Mouse
556
384
143
7
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to APC-Cy7
128
113
91
54
29
28
26
23
22
22
17
16
12
11
11
11
11
11
10
8
8
8
8
8
7
7
6
5
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
892
154
148
145
108
83
82
69
47
21
19
17
16
14
13
12
9
7
6
6
5
4
3
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2660659
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2660324 IP IHC ELISA WB PerCP-Cy5.5 Mouse IgG1 kappa Log in to see BC96 4
1 ABIN2660057 FACS IP IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see M-A251 3
1 ABIN187250 IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see IL2R-1
1 ABIN120760 FACS IP Mouse IgM Log in to see MEM-140
1 ABIN2660660 IP WB APC-Cy7 Mouse IgG1 kappa Log in to see BC96 5
1 ABIN2660325 IP IHC ELISA WB PerCP-Cy5.5 Mouse IgG1 kappa Log in to see BC96 4
1 ABIN2660058 FACS IP IHC PerCP-Cy5.5 Mouse IgG1 kappa Log in to see M-A251 3
1 ABIN94049 FACS IP Mouse IgM Log in to see MEM-140 3
1 ABIN94050 FACS IP Biotin Mouse IgM Log in to see MEM-140 3
1 ABIN4291008 FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 kappa Log in to see 2R12
1 ABIN303574 FACS IP Biotin Mouse IgM Log in to see MEM-140
1 ABIN1859412 ICC IHC IP WB Rabbit IgG AA 22-213 Log in to see Polyclonal
1 ABIN207006 FACS IP Mouse IgM Log in to see MEM-140
1 ABIN259702 FACS IP Biotin Mouse IgM Log in to see MEM-140
1 ABIN269086 CyTOF FACS IP Mouse IgM Log in to see MEM-140
1 ABIN3199653 FACS IP Biotin Mouse IgM Log in to see MEM-140
1 ABIN3199652 FACS IP Mouse IgM Log in to see MEM-140
1 ABIN2224491 IP HRP Mouse IgM Log in to see 14D28
1 ABIN2224515 FACS IP Alkaline Phosphatase (AP) Mouse IgM Log in to see 14D28
1 ABIN2224488 FACS IP APC Mouse IgM Log in to see 14D28

Similar anti-CD25 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 4 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 4 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
ELISA 60 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 410 Antibodies
Functional Studies (Func) 13 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 63 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(561), (444), (152), (17), (14), (9), (8), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(556), (384), (143), (7), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to APC-Cy7
(128), (113), (91), (54), (29), (28), (26), (23), (22), (22), (17), (16), (12), (11), (11), (11), (11), (11), (10), (8), (8), (8), (8), (8), (7), (7), (6), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(892), (154), (148), (145), (108), (83), (82), (69), (47), (21), (19), (17), (16), (14), (13), (12), (9), (7), (6), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2660659) Images
Cross-Reactivity Baboon, Chimpanzee, Cynomolgus, Rhesus Monkey, Pigtailed Macaque
Purification The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Clone BC96
Isotype IgG1 kappa

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2660659) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2660659) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2660659) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD25 Antibody (ABIN2660659)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Cornwell, Lewis, Fan, Rappaport, Rogers: "Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques." in: Journal of neuroimmunology, Vol. 265, Issue 1-2, pp. 43-50, 2013

Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009

Ernst, Lee, Nakanishi, Karimbux, Rezende, Stashenko, Seki, Taubman, Kawai: "Diminished forkhead box P3/CD25 double-positive T regulatory cells are associated with the increased nuclear factor-kappaB ligand (RANKL+) T cells in bone resorption lesion of periodontal disease." in: Clinical and experimental immunology, Vol. 148, Issue 2, pp. 271-80, 2007

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2660659) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (APC-Cy7) (ABIN2660659) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (APC-Cy7)