You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) CD25 antibody for Mass Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • HEM45
  • IDDM10
  • IL-2RA
  • Il2r
  • IL2R
  • IL2RAC
  • Ly-43
  • TCGFR
Reactivity
Mouse (Murine)
569
456
169
19
10
10
8
5
4
3
3
2
1
1
Host
Rat
602
405
134
5
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to Alexa Fluor 647
130
124
101
61
30
28
25
24
22
19
17
15
14
12
12
12
12
12
12
12
10
10
10
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Mass Cytometry (CyTOF)
936
145
141
129
127
121
80
72
48
21
18
18
17
11
10
9
6
6
5
4
3
3
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2657710
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008650115 ABIN2657711 FACS CyTOF Alexa Fluor 647 Rat IgG1 lambda Log in to see PC61 12

Top referenced anti-CD25 antibody for Mass Cytometry

Similar anti-CD25 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 16 Antibodies
Blocking Reagent (BR) 4 Antibodies
Cytotoxicity Test (CyTox) 1 Antibodies
ELISA 15 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Flow Cytometry (FACS) 403 Antibodies
Functional Studies (Func) 28 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 51 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 90 Antibodies
In vitro Assay (in vitro) 4 Antibodies
Intracellular Flow Cytometry (ICFC) 17 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(569), (456), (169), (19), (10), (10), (8), (5), (4), (3), (3), (2), (1), (1)
Host Rat
(602), (405), (134), (5), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to Alexa Fluor 647
(130), (124), (101), (61), (30), (28), (25), (24), (22), (19), (17), (15), (14), (12), (12), (12), (12), (12), (12), (12), (10), (10), (10), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Mass Cytometry (CyTOF)
(936), (145), (141), (129), (127), (121), (80), (72), (48), (21), (18), (18), (17), (11), (10), (9), (6), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 12 references available
Supplier Log in to see

Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2657710) Images
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Clone PC61
Isotype IgG1 lambda

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 Antibody (ABIN2657710) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 Antibody (ABIN2657710) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 Antibody (ABIN2657710) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD25 Antibody (ABIN2657710)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

León-Ponte, Ahern, OConnell: "Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor." in: Blood, Vol. 109, Issue 8, pp. 3139-46, 2007

Lei, Scott: "Induction of tolerance to factor VIII inhibitors by gene therapy with immunodominant A2 and C2 domains presented by B cells as Ig fusion proteins." in: Blood, Vol. 105, Issue 12, pp. 4865-70, 2005

Pasare, Medzhitov: "Toll-dependent control mechanisms of CD4 T cell activation." in: Immunity, Vol. 21, Issue 5, pp. 733-41, 2004

Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, 2000

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, 2000

Onizuka, Tawara, Shimizu, Sakaguchi, Fujita, Nakayama: "Tumor rejection by in vivo administration of anti-CD25 (interleukin-2 receptor alpha) monoclonal antibody." in: Cancer research, Vol. 59, Issue 13, pp. 3128-33, 1999

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Moreau, Nabholz, Diamantstein, Malek, Shevach, Thèze: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987

Lowenthal, Corthésy, Tougne, Lees, MacDonald, Nabholz: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, 1985

Lowenthal, Zubler, Nabholz, MacDonald: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, 1985

Ceredig, Lowenthal, Nabholz, MacDonald: "Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells." in: Nature, Vol. 314, Issue 6006, pp. 98-100, 1985

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 Antibody (ABIN2657710) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD25 antibody (Interleukin 2 Receptor, alpha)  (Alexa Fluor 647) (ABIN2657710) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (Alexa Fluor 647)